Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	511,672	T→C	V11A (GTG→GCG)	glsA →	glutaminase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	511,672	0	T	C	100.0%	105.9 / NA	31	V11A (GTG→GCG)	glsA	glutaminase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

AGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCG  >  NC_000913/511549‑511805                                                                                                                            |                                                                                                                                      aGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACAcc                                                                                                                       <  2:283442/140‑1 (MQ=255)       gggTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAAttt                                                                                                                 >  1:711401/1‑140 (MQ=255)        ggTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAGACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTtc                                                                                                                >  2:568530/1‑140 (MQ=255)        ggTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACAcc                                                                                                                       >  2:713352/1‑133 (MQ=255)        ggTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACAcc                                                                                                                       <  1:713352/133‑1 (MQ=255)               aGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTcactcact                                                                                                         >  1:106361/1‑140 (MQ=255)               aGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTcactcact                                                                                                         <  2:106361/140‑1 (MQ=255)                          gATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGAc                                                                                              >  2:473254/1‑140 (MQ=255)                           aTGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACa                                                                                             >  1:480732/1‑140 (MQ=255)                              ccTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACaaaa                                                                                          >  1:681880/1‑140 (MQ=255)                                   aaaTCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAGCAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCg                                                                                     <  2:447556/140‑1 (MQ=255)                                    aaTCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGa                                                                                    >  2:628345/1‑140 (MQ=255)                                                    aGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTg                                                                    <  2:480732/140‑1 (MQ=255)                                                            ccTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAGACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGt                                                            <  1:568530/140‑1 (MQ=255)                                                                ccGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCa                                                        >  2:356154/1‑140 (MQ=255)                                                                              aGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTggcggc                                          >  1:708331/1‑140 (MQ=255)                                                                              aGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTggcggc                                          <  1:732447/140‑1 (MQ=255)                                                                              aGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTggcggc                                          <  2:681880/140‑1 (MQ=255)                                                                                 aGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGt                                       >  2:41445/1‑140 (MQ=255)                                                                                   ttAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTgg                                     >  2:703078/1‑140 (MQ=255)                                                                                     aaCAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCt                                   >  2:721251/1‑140 (MQ=255)                                                                                            gATGTTAGATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGa                            <  1:310934/140‑1 (MQ=255)                                                                                                   gATGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTATCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGa                     <  1:628345/140‑1 (MQ=255)                                                                                                     tGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATg                   <  2:148929/140‑1 (MQ=255)                                                                                                     tGCAAACAAATTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATg                   <  1:356154/140‑1 (MQ=255)                                                                                                       caaacaaaTTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGc                 <  1:64876/140‑1 (MQ=255)                                                                                                       caaacaaaTTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGc                 >  2:64876/1‑140 (MQ=255)                                                                                                          acaaaTTACAGCAGGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAAc              <  1:261683/140‑1 (MQ=255)                                                                                                                   agcagGCAGCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGt     >  1:448631/1‑140 (MQ=255)                                                                                                                      aggcagCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCg  >  2:379851/1‑140 (MQ=255)                                                                                                                      aggcagCGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCg  <  1:41445/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                      AGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCG  >  NC_000913/511549‑511805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑