Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	592,834	A→G	R115R (CGT→CGC)	nfrB ←	bacteriophage N4 receptor, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	592,834	0	A	G	100.0%	106.2 / NA	31	R115R (CGT→CGC)	nfrB	bacteriophage N4 receptor, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/13);  total (18/13)

CTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCACGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACC  >  NC_000913/592709‑592957                                                                                                                              |                                                                                                                            cTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCggg                                                                                                               <  2:760559/140‑1 (MQ=255)  tACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCgggg                                                                                                              <  2:682089/140‑1 (MQ=255)               ggTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAgg                                                                                                 >  1:167291/1‑140 (MQ=255)                   aTGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTg                                                                                               >  1:30845/1‑138 (MQ=255)                   aTGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTg                                                                                               <  2:30845/138‑1 (MQ=255)                      gCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCcaac                                                                                          <  1:220613/140‑1 (MQ=255)                       cGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCcaaca                                                                                         >  1:738718/1‑140 (MQ=255)                         tCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGt                                                                                                <  2:597556/131‑1 (MQ=255)                         tCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGt                                                                                                >  1:597556/1‑131 (MQ=255)                                   tgttCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAg                                                                             >  2:764256/1‑140 (MQ=255)                                   tgttCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAg                                                                             >  2:82598/1‑140 (MQ=255)                                               cGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCg                                                                 >  1:219913/1‑140 (MQ=255)                                               cGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCg                                                                 >  1:293390/1‑140 (MQ=255)                                                       cTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGt                                                         <  2:738718/140‑1 (MQ=255)                                                               gCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCa                                                 >  1:433885/1‑140 (MQ=255)                                                                  aGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCt                                              <  2:167291/140‑1 (MQ=255)                                                                         gcgcAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCgg                                           <  2:735877/136‑1 (MQ=255)                                                                         gcgcAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCgg                                           >  1:735877/1‑136 (MQ=255)                                                                         gcgcAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGACAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCt                                              >  2:430220/1‑133 (MQ=255)                                                                         gcgcAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGACAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCt                                              <  1:430220/133‑1 (MQ=255)                                                                          cgcAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCata                                      >  1:554580/1‑140 (MQ=255)                                                                                       aTGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGc                         <  1:395312/140‑1 (MQ=255)                                                                                             aTTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGttt                   >  1:714252/1‑140 (MQ=255)                                                                                             aTTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGttt                   >  2:507097/1‑140 (MQ=255)                                                                                             aTTCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGttt                   >  1:35517/1‑140 (MQ=255)                                                                                               tCGGGAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCa                 >  2:94673/1‑140 (MQ=255)                                                                                                  ggAAGCGAGCGCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTc              >  2:412070/1‑140 (MQ=255)                                                                                                         agcgCACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCg       >  2:150646/1‑140 (MQ=255)                                                                                                          gcgcACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCgg      <  1:507097/140‑1 (MQ=255)                                                                                                            gcACACTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGaa    <  1:94673/140‑1 (MQ=255)                                                                                                              acacTTCGTCAACATCGCGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAAcc  <  2:433885/140‑1 (MQ=255)                                                                                                                              |                                                                                                                            CTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGACGCGCGCAGACTACCTTATGCACATTCGGGAAGCGAGCGCACACTTCGTCAACATCACGCTGAGTATCGGGGTCGTTGGGGTAGGTGCCAACAAAGATATGATAGTTTTCGTAGTCGAGCGTGGTCGCCGCCAGCTCGGCCATATTGCCGATGACGCCCGTTTCATTCCACGCCGGAACC  >  NC_000913/592709‑592957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑