Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	776,687	C→T	Q116* (CAG→TAG)	tolA →	Tol‑Pal system protein TolA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	776,687	0	C	T	100.0%	101.3 / NA	30	Q116* (CAG→TAG)	tolA	Tol‑Pal system protein TolA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

TCAAGCGCGAAGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTGCGGAAGAAGCAGC  >  NC_000913/776555‑776820                                                                                                                                     |                                                                                                                                      tCAAGCGCGAAGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGaaaaa                                                                                                                                <  2:351756/140‑1 (MQ=255)   aaGCGCGAAGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGc                                                                                                                              <  2:550245/140‑1 (MQ=255)             cGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTgaaga                                                                                                                    <  2:181229/140‑1 (MQ=255)                            cgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTgaaga                                                                                                                    >  2:398358/1‑125 (MQ=255)                            cgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTgaaga                                                                                                                    <  1:398358/125‑1 (MQ=255)                            cgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGc                                                                                                              <  1:117490/131‑1 (MQ=255)                            cgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGc                                                                                                              >  2:117490/1‑131 (MQ=255)                                   tGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTa                                                                                              <  2:665795/140‑1 (MQ=255)                                      aGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACGGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAg                                                                                           >  1:625295/1‑140 (MQ=255)                                           cagcagGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGaa                                                                                      <  2:158327/140‑1 (MQ=255)                                            agcagGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGaag                                                                                     <  2:719900/140‑1 (MQ=255)                                                     ctgAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGaggc                                                                                    >  2:695848/1‑132 (MQ=255)                                                     ctgAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGaagc                                                                                    <  1:695848/132‑1 (MQ=255)                                                               gCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAg                                                                  >  2:752726/1‑140 (MQ=255)                                                                                        gAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGc                                         >  2:464485/1‑140 (MQ=255)                                                                                        gAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGc                                         >  2:324277/1‑140 (MQ=255)                                                                                             ccTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGaa                                      <  2:778419/138‑1 (MQ=255)                                                                                             ccTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGaa                                      >  1:778419/1‑138 (MQ=255)                                                                                             ccTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAgg                                    >  1:24530/1‑140 (MQ=255)                                                                                                     aaCTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCa                            <  1:464485/140‑1 (MQ=255)                                                                                                       cTTGAGAAAGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGa                          >  2:684369/1‑140 (MQ=255)                                                                                                              aaGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTaaa                   >  1:465044/1‑140 (MQ=255)                                                                                                              aaGAGCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTaaa                   <  1:381113/140‑1 (MQ=255)                                                                                                               agagCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAg                  <  1:324277/140‑1 (MQ=255)                                                                                                                  gCGGTTAGCGGCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTg               >  2:322130/1‑140 (MQ=255)                                                                                                                            gCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTGCGGAAGAagc     <  1:588289/140‑1 (MQ=255)                                                                                                                            gCTCAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTGCGGAAGAagc     <  1:203147/140‑1 (MQ=255)                                                                                                                               cAGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTGCGGAAGAagcagc  >  1:625168/1‑140 (MQ=255)                                                                                                                                aGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCt                >  2:329966/1‑125 (MQ=255)                                                                                                                                aGGAGTAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCt                <  1:329966/125‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      TCAAGCGCGAAGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTGCGGAAGAAGCAGC  >  NC_000913/776555‑776820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑