Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,986	A→G	intergenic (+68/‑82)	lysW → / → valZ	tRNA‑Lys/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,986	0	A	G	100.0%	142.6 / NA	42	intergenic (+68/‑82)	lysW/valZ	tRNA‑Lys/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/26);  total (16/26)

GTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGG  >  NC_000913/780857‑781117                                                                                                                                  |                                                                                                                                    gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAg                                                                                                                           <  2:735390/140‑1 (MQ=255)          gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGc                                                                                                                  >  1:339195/1‑140 (MQ=255)           cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCa                                                                                                                 >  2:543756/1‑140 (MQ=255)            aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCAc                                                                                                                >  2:230849/1‑140 (MQ=255)              ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGa                                                                                                              >  2:471499/1‑140 (MQ=255)               tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGAc                                                                                                             >  1:334302/1‑140 (MQ=255)                gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGAcc                                                                                                            >  1:635321/1‑140 (MQ=255)                  cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACcca                                                                                                          <  1:471499/140‑1 (MQ=255)                    tttAATCAATTGGTCGAAGGTTCGAATCGTGCACGACCCACCAGTTTTAACAGCGAAGGCAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACccacc                                                                                                        <  2:450547/140‑1 (MQ=14)                          cAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAAt                                                                                                  <  1:429620/140‑1 (MQ=255)                           aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATg                                                                                                 >  2:92890/1‑140 (MQ=255)                             ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGAc                                                                                               >  2:390838/1‑140 (MQ=255)                                         tCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGt                                                                                   <  2:334302/140‑1 (MQ=255)                                                 tGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAagtag                                                                           >  2:3135/1‑140 (MQ=255)                                                  gCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAagtagt                                                                          <  1:567871/140‑1 (MQ=255)                                                  gCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAagtagt                                                                          <  1:92890/140‑1 (MQ=255)                                                  gCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAagtagt                                                                          <  2:127114/140‑1 (MQ=255)                                                           accaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTAt                                                                 >  2:2685/1‑140 (MQ=255)                                                            ccaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATc                                                                <  1:390838/140‑1 (MQ=255)                                                            ccaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATc                                                                <  2:562293/140‑1 (MQ=255)                                                            ccaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATc                                                                <  2:471346/140‑1 (MQ=255)                                                            ccaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATc                                                                <  1:3135/140‑1 (MQ=255)                                                            ccaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATc                                                                <  2:635321/140‑1 (MQ=255)                                                              aGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCa                                                              >  1:446902/1‑140 (MQ=255)                                                                ttttAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAgc                                                            <  1:230849/140‑1 (MQ=255)                                                                  ttAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAgcgc                                                          <  1:543756/140‑1 (MQ=255)                                                                         cGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATc                                                    >  2:176689/1‑139 (MQ=255)                                                                         cGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATc                                                    <  1:176689/139‑1 (MQ=255)                                                                                     ttAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTc                                       <  2:339195/140‑1 (MQ=255)                                                                                            tgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTggg                                >  2:734491/1‑140 (MQ=255)                                                                                             gtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGga                               <  1:55153/140‑1 (MQ=255)                                                                                                 gATAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGc                           >  1:384823/1‑140 (MQ=255)                                                                                                  aTAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCa                          <  2:769884/140‑1 (MQ=255)                                                                                                  aTAACGTTGCGTCAGCAAC‑GCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                         <  2:344577/140‑1 (MQ=255)                                                                                                   tAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                         <  1:734491/140‑1 (MQ=255)                                                                                                   tAACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                         <  1:2685/140‑1 (MQ=255)                                                                                                     aCGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCt                       <  1:615238/140‑1 (MQ=255)                                                                                                      cGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGagag                            >  1:690016/1‑134 (MQ=255)                                                                                                      cGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGagag                            <  2:690016/134‑1 (MQ=255)                                                                                                         tGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGc                           >  1:248264/1‑132 (MQ=255)                                                                                                         tGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGc                           <  2:248264/132‑1 (MQ=255)                                                                                                                          gTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcgg  <  2:446902/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    GTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGG  >  NC_000913/780857‑781117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑