Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	48,716	A→G	R316R (CGA→CGG)	kefC →	K(+) : H(+) antiporter KefC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	48,716	0	A	G	100.0%	157.8 / NA	46	R316R (CGA→CGG)	kefC	K(+) : H(+) antiporter KefC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/22);  total (24/22)

TGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACC  >  NC_000913/48580‑48851                                                                                                                                         |                                                                                                                                        tGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCg                                                                                                                                      >  1:644423/1‑140 (MQ=255) tGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCg                                                                                                                                      >  2:437510/1‑140 (MQ=255)       tGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCaa                                                                                                                                <  1:237362/140‑1 (MQ=255)         gggCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGt                                                                                                                              <  1:437510/140‑1 (MQ=255)         gggCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGt                                                                                                                              <  2:644423/140‑1 (MQ=255)           gCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGc                                                                                                                            >  1:223543/1‑140 (MQ=255)              gTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCAAGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCaa                                                                                                                         <  1:193863/140‑1 (MQ=255)                    cATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAAc                                                                                                                   >  1:544800/1‑140 (MQ=255)                            ttGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGcgtcgt                                                                                                           >  1:440132/1‑140 (MQ=255)                                 aTGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGtt                                                                                                      <  2:544800/140‑1 (MQ=255)                                  tGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGttt                                                                                                     <  1:175709/140‑1 (MQ=255)                                       aTAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGgt                                                                                                <  2:440132/140‑1 (MQ=255)                                         aGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGgtgt                                                                                              >  1:133464/1‑140 (MQ=255)                                            cTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGtgttgt                                                                                           <  2:374246/140‑1 (MQ=255)                                            cTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGtgttgt                                                                                           <  2:133464/140‑1 (MQ=255)                                             tttGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTgttgtt                                                                                          >  2:343680/1‑140 (MQ=255)                                              ttGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTa                                                                                         <  1:128013/140‑1 (MQ=255)                                              ttGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTa                                                                                         >  1:378104/1‑140 (MQ=255)                                              ttGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTa                                                                                         >  1:144483/1‑140 (MQ=255)                                               tGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTag                                                                                        >  1:91435/1‑140 (MQ=255)                                                    cGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTagggcag                                                                                   >  2:391234/1‑140 (MQ=255)                                                     gctgctTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGgt                                                                                                >  1:101633/1‑126 (MQ=255)                                                     gctgctTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGgt                                                                                                <  2:101633/126‑1 (MQ=255)                                                     gctgctTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTagggcagg                                                                                  >  2:27219/1‑140 (MQ=255)                                                       tgctTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACGGCGTCGTTGGTTTGCGGTGTTGTTagggcagggc                                                                                >  1:424387/1‑140 (MQ=255)                                                        gctTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAgggcagggca                                                                               <  1:391234/140‑1 (MQ=255)                                                        gctTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAgggcagggca                                                                               <  1:27219/140‑1 (MQ=255)                                                           tGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGCGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTg                                                                            >  2:153917/1‑140 (MQ=255)                                                               aaCCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGtt                                                                        <  2:91435/140‑1 (MQ=255)                                                               aaCCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGtt                                                                        <  1:153917/140‑1 (MQ=255)                                                                 cccATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTg                                                                      <  2:378104/140‑1 (MQ=255)                                                                  ccATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGc                                                                     <  2:223543/140‑1 (MQ=255)                                                                   cATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTaggg                                                                                      <  1:584085/122‑1 (MQ=255)                                                                   cATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTaggg                                                                                      >  2:584085/1‑122 (MQ=255)                                                                   cATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGCTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTCAGTTTGcc                                                                    >  1:56811/1‑140 (MQ=255)                                                                            ttGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTa                                                           >  1:217709/1‑140 (MQ=255)                                                                                    ttGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTggcgc                                                   <  1:318295/140‑1 (MQ=255)                                                                                                                      tGCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGTGAATGTGCTGGAGCCGGAGTgg                 >  2:115359/1‑140 (MQ=255)                                                                                                                       gCTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGTCGGAGTggg                <  2:217709/140‑1 (MQ=255)                                                                                                                        cTGTGGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGc               >  1:187109/1‑140 (MQ=255)                                                                                                                           tgGCTGATTGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGaa            >  1:460049/1‑140 (MQ=255)                                                                                                                                  ttGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCt      >  1:459296/1‑139 (MQ=255)                                                                                                                                  ttGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCt      <  2:459296/139‑1 (MQ=255)                                                                                                                                  ttGCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTg     >  1:574331/1‑140 (MQ=255)                                                                                                                                    gCCCGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGAc   <  2:187109/140‑1 (MQ=255)                                                                                                                                     cccGGCCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGAcc  >  1:768709/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TGCTGTTGGGGCTGTTTTTCATCGGTGTTGGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACC  >  NC_000913/48580‑48851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑