Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	865,679	T→A	D229V (GAT→GTT)	moeA ←	molybdopterin molybdotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	865,679	0	T	A	100.0%	84.4 / NA	25	D229V (GAT→GTT)	moeA	molybdopterin molybdotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/13);  total (12/13)

AGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTA  >  NC_000913/865545‑865813                                                                                                                                       |                                                                                                                                       aGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAacgtcg                                                                                                                                   >  1:776344/1‑140 (MQ=255)     ggCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGa                                                                                                                               <  1:713214/140‑1 (MQ=255)      gCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGAt                                                                                                                              <  2:776344/140‑1 (MQ=255)         aTCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAAt                                                                                                                           >  2:147249/1‑140 (MQ=255)              ccccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTa                                                                                                                      <  2:455009/140‑1 (MQ=255)               cccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTaa                                                                                                                     >  2:28975/1‑140 (MQ=255)                                 gTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATcc                                                                                                   >  1:510352/1‑140 (MQ=255)                                            aTCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGtt                                                                                           <  2:29888/137‑1 (MQ=255)                                            aTCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGtt                                                                                           >  1:29888/1‑137 (MQ=255)                                              ccGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTagc                                                                                      <  1:783293/140‑3 (MQ=255)                                                  cTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCa                                                                                  >  2:432146/1‑140 (MQ=255)                                                      cccACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGgtg                                                                              >  2:168000/1‑140 (MQ=255)                                                      cccACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGgtg                                                                              <  1:305802/140‑1 (MQ=255)                                                             aaaCACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCg                                                                       <  1:147249/140‑1 (MQ=255)                                                              aaCACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCga                                                                      >  1:734427/1‑140 (MQ=255)                                                               acacCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCgag                                                                     <  2:510352/140‑1 (MQ=255)                                                                     ccgGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGtt                                                               >  2:710626/1‑140 (MQ=255)                                                                             gATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGt                                                       >  2:306707/1‑140 (MQ=255)                                                                                           ccTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCg                                         >  2:168293/1‑140 (MQ=255)                                                                                              ggCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGccc                                      <  1:710626/140‑1 (MQ=255)                                                                                                        ttCAATAAATGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGAc                            <  1:469972/140‑1 (MQ=255)                                                                                                                 tGCGGCGCGCAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACt                   >  2:130242/1‑140 (MQ=255)                                                                                                                     gcgcgcAGGGCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAg               >  1:55784/1‑140 (MQ=255)                                                                                                                             ggCATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCAc       <  1:168000/140‑1 (MQ=255)                                                                                                                               cATGGGGAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCg     <  1:181662/140‑1 (MQ=255)                                                                                                                                  ggggAACGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTa  <  2:55784/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTA  >  NC_000913/865545‑865813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑