Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	950,062	A→G	D132G (GAC→GGC)	ycaK →	putative NAD(P)H‑dependent oxidoreductase YcaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	950,062	0	A	G	100.0%	315.7 / NA	90	D132G (GAC→GGC)	ycaK	putative NAD(P)H‑dependent oxidoreductase YcaK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (53/37);  total (53/37)

TTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGACAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTGGTATTCGATGGGGAAGAACTT  >  NC_000913/949927‑950198                                                                                                                                        |                                                                                                                                         ttCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCaaa                                                                                                                                      >  1:97237/1‑140 (MQ=255)        ctGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCAt                                                                                                                               <  1:534613/140‑1 (MQ=255)                   gCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATg                                                                                                                    >  2:212007/1‑140 (MQ=255)                      tCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTc                                                                                                                          >  2:172154/1‑131 (MQ=255)                      tCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTc                                                                                                                          <  1:172154/131‑1 (MQ=255)                       cccGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGTGGCAAAGAATCATTTGTCCAGATGGGCt                                                                                                                >  1:523909/1‑140 (MQ=255)                       cccGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCt                                                                                                                <  2:515060/140‑1 (MQ=255)                        ccGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTg                                                                                                                            <  1:270992/127‑1 (MQ=255)                        ccGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTg                                                                                                                            >  2:270992/1‑127 (MQ=255)                                 cTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGgaaaaaa                                                                                                      >  1:285870/1‑140 (MQ=255)                                         atatatTGACAGAGTATGGAATAGTGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCg                                                                                              >  1:658160/1‑140 (MQ=255)                                          tatatTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGa                                                                                             >  1:196824/1‑140 (MQ=255)                                          tatatTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGa                                                                                             >  2:446060/1‑140 (MQ=255)                                                   agagTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTaaa                                                                                    >  1:20689/1‑140 (MQ=255)                                                     agTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTaaaaa                                                                                  >  2:149992/1‑140 (MQ=255)                                                      gTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTaaaaaa                                                                                 >  2:693515/1‑140 (MQ=255)                                                       tATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAat                                                                                >  1:37216/1‑140 (MQ=255)                                                       tATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAat                                                                                >  2:787600/1‑140 (MQ=255)                                                       tATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAat                                                                                >  1:123395/1‑140 (MQ=255)                                                          ggAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATAtg                                                                             <  2:453171/140‑1 (MQ=255)                                                          ggAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATAtg                                                                             <  2:196824/140‑1 (MQ=255)                                                          ggAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATAtg                                                                             <  2:123395/140‑1 (MQ=255)                                                           gAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATAtgt                                                                            <  1:158410/140‑1 (MQ=255)                                                            aataatGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGGAAAAAATATAAGCGATTATTTAAAAAATAtgtg                                                                           <  2:285870/140‑1 (MQ=255)                                                               aatGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAg                                                                        >  2:131139/1‑140 (MQ=255)                                                               aatGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAg                                                                        >  1:652535/1‑140 (MQ=255)                                                               aatGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAg                                                                        >  2:43681/1‑140 (MQ=255)                                                               aatGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAg                                                                        >  2:99073/1‑140 (MQ=255)                                                               aatGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAg                                                                        >  1:43112/1‑140 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGTAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGtt                                                                      >  1:703183/1‑140 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCa                                                                         >  1:274617/1‑137 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCa                                                                         <  2:274617/137‑1 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCa                                                                         <  1:606563/137‑1 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCa                                                                         >  2:606563/1‑137 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGtt                                                                      >  2:372194/1‑140 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGtt                                                                      >  1:399444/1‑140 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGtt                                                                      >  1:802929/1‑140 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGtt                                                                      >  2:40981/1‑140 (MQ=255)                                                                 tGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGtt                                                                      >  1:522258/1‑140 (MQ=255)                                                                   ggctggctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTAt                                                                    <  2:747710/140‑1 (MQ=255)                                                                   ggctggctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTAt                                                                    >  1:331283/1‑140 (MQ=255)                                                                     ctggctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCt                                                                  <  2:399444/140‑1 (MQ=255)                                                                     ctggctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCt                                                                  <  2:97237/140‑1 (MQ=255)                                                                     ctggctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCt                                                                  >  2:282810/1‑140 (MQ=255)                                                                     ctggctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCt                                                                  <  2:295877/140‑1 (MQ=255)                                                                      tggctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCtt                                                                 >  2:265629/1‑140 (MQ=255)                                                                        gctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTgg                                                               >  2:642118/1‑140 (MQ=255)                                                                        gctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTgg                                                               >  2:455368/1‑140 (MQ=255)                                                                        gctTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTgg                                                               >  2:304262/1‑140 (MQ=255)                                                                          ttATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTa                                                             <  2:522258/140‑1 (MQ=255)                                                                           tATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTAt                                                            >  1:329493/1‑140 (MQ=255)                                                                              ggAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGa                                                         <  2:43112/140‑1 (MQ=255)                                                                               gagaTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGaa                                                        <  2:140908/140‑1 (MQ=255)                                                                                  aTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGCGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGAt                                                     >  1:346984/1‑140 (MQ=255)                                                                                  aTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCt                                                                  <  2:189315/127‑1 (MQ=255)                                                                                  aTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCt                                                                  >  1:189315/1‑127 (MQ=255)                                                                                  aTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGAt                                                     >  2:172354/1‑140 (MQ=255)                                                                                  aTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGAt                                                     >  2:457430/1‑140 (MQ=255)                                                                                  aTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGAt                                                     >  2:531032/1‑140 (MQ=255)                                                                                  aTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGAt                                                     >  2:639749/1‑140 (MQ=255)                                                                                  aTGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCT‑GGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATg                                                    >  2:728813/1‑140 (MQ=255)                                                                                     ggCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGcc                                                  >  2:228245/1‑140 (MQ=255)                                                                                       cacaAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGa                                                >  1:269654/1‑140 (MQ=255)                                                                                         caAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATg                                              <  2:802929/140‑1 (MQ=255)                                                                                          aaaTTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGt                                             >  2:474777/1‑140 (MQ=255)                                                                                            aTTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCa                                           <  1:40981/140‑1 (MQ=255)                                                                                                ccATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACttt                                       >  2:41608/1‑140 (MQ=255)                                                                                                ccATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCT‑GGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  1:728813/140‑1 (MQ=255)                                                                                                 cATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  1:149992/140‑1 (MQ=255)                                                                                                 cATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  1:304262/140‑1 (MQ=255)                                                                                                 cATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      >  1:477353/1‑140 (MQ=255)                                                                                                 cATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  1:474777/140‑1 (MQ=255)                                                                                                 cATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  2:32083/140‑1 (MQ=255)                                                                                                 cATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  2:269654/140‑1 (MQ=255)                                                                                                 cATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  1:372194/140‑1 (MQ=255)                                                                                                 cATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  1:41608/140‑1 (MQ=255)                                                                                                   ttCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCtt                                    <  1:455368/140‑1 (MQ=255)                                                                                                      aaTAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTtgtg                                 <  1:446060/140‑1 (MQ=255)                                                                                                           aGTTCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATa                            <  1:693515/140‑1 (MQ=255)                                                                                                             ttCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACa                          >  1:82744/1‑140 (MQ=255)                                                                                                              tCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      <  2:670488/127‑1 (MQ=255)                                                                                                              tCGTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTc                                      >  1:670488/1‑127 (MQ=255)                                                                                                                gTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTg                       >  2:354544/1‑140 (MQ=255)                                                                                                                gTTGGGTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTg                       >  2:108427/1‑140 (MQ=255)                                                                                                                     gTGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTGGTAtt                  <  1:172354/140‑1 (MQ=255)                                                                                                                      tGGCGCTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTGGTATTc                 >  2:410522/1‑140 (MQ=255)                                                                                                                        gcgcTGGTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTGGTATTCGa               <  1:486774/140‑1 (MQ=255)                                                                                                                              gTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCTCTTTCTTGTGTAATACAGTGGTATTCGATGGGga         <  2:346984/140‑1 (MQ=255)                                                                                                                              gTTGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTGGTATTCGATGGGga         >  1:562555/1‑140 (MQ=255)                                                                                                                                tGGAGGAGGCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTGGTATTCGATGGGgaag       <  1:410522/140‑1 (MQ=255)                                                                                                                                     gaggCAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTGGTATTCGATGGGGAAGAACtt  <  1:531032/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTCAATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGACAAAGAATCATTTGTCCAGATGGGCTGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGATGCCGATGTCACTTTCTTGTGTAATACAGTGGTATTCGATGGGGAAGAACTT  >  NC_000913/949927‑950198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑