Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,128,342	C→T	N168N (AAC→AAT)	murJ →	putative lipid II flippase MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,128,342	0	C	T	100.0%	128.0 / NA	37	N168N (AAC→AAT)	murJ	putative lipid II flippase MurJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/20);  total (17/20)

ATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCAT  >  NC_000913/1128207‑1128476                                                                                                                                        |                                                                                                                                       aTTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCa                                                                                                                                    <  1:718155/140‑1 (MQ=255)        cTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGAt                                                                                                                             <  1:295150/140‑1 (MQ=255)                  agcTACTAAAGATTACCTTTCCCTATATCTTGTTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGGTCCAACACTGCTTAATATCAGCATGATTGGTTTcgcg                                                                                                                   <  2:709543/140‑1 (MQ=255)                     tactaAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTg                                                                                                                >  2:315913/1‑140 (MQ=255)                              ttACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCa                                                                                                       >  1:173432/1‑140 (MQ=255)                               tACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAc                                                                                                      <  2:762587/140‑1 (MQ=255)                                aCCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAcc                                                                                                     >  1:97841/1‑140 (MQ=255)                                 ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                                                                                    >  2:558278/1‑140 (MQ=255)                                 ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                                                                                    >  1:206631/1‑140 (MQ=255)                                      cccTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACtt                                                                                               >  1:217623/1‑140 (MQ=255)                                              cTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCAc                                                                                       <  2:58865/140‑1 (MQ=255)                                              cTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCAc                                                                                       <  1:315913/140‑1 (MQ=255)                                                tGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCg                                                                                     >  1:483696/1‑140 (MQ=255)                                                tGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCg                                                                                     >  2:319876/1‑140 (MQ=255)                                                  cTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCAc                                                                                       >  1:324273/1‑136 (MQ=255)                                                  cTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCAc                                                                                       <  2:324273/136‑1 (MQ=255)                                                    gATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgc                                                                                 <  1:558278/140‑1 (MQ=255)                                                         cccTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcg                                                                            >  1:799327/1‑140 (MQ=255)                                                              gCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcgctggc                                                                       <  1:350312/140‑1 (MQ=255)                                                               cGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGctggcgctggcg                                                                      <  1:287440/140‑1 (MQ=255)                                                                   gCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTggg                                                                  <  2:217623/140‑1 (MQ=255)                                                                    cTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGc                                                                 >  1:194269/1‑140 (MQ=255)                                                                    cTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGc                                                                 >  1:225306/1‑140 (MQ=255)                                                                                 ttCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTcggc                                                    >  1:199562/1‑140 (MQ=255)                                                                                           gTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGc                                          >  1:208969/1‑140 (MQ=255)                                                                                                aCCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTg                                     <  2:206631/140‑1 (MQ=255)                                                                                                       ctcGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATc                              <  1:121180/140‑1 (MQ=255)                                                                                                        tcGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCa                             <  2:225306/140‑1 (MQ=255)                                                                                                             tCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTAc                        <  2:85092/140‑1 (MQ=255)                                                                                                               cGGCGTTTGCTCCAACAGTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCg                      >  1:603556/1‑140 (MQ=255)                                                                                                                  cGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCAc                   <  2:30649/140‑1 (MQ=255)                                                                                                                       gCTCCAACAGTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTgaa              <  2:603556/140‑1 (MQ=255)                                                                                                                          ccAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTgaagaa           <  2:173432/140‑1 (MQ=255)                                                                                                                              cacTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATc       >  1:717048/1‑140 (MQ=255)                                                                                                                               acTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCg      <  2:527440/140‑1 (MQ=255)                                                                                                                                cTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCgg     <  2:97841/140‑1 (MQ=255)                                                                                                                                   cTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCAt  >  1:674635/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       ATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCAT  >  NC_000913/1128207‑1128476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑