Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,140	T→C	T410A (ACT→GCT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,140	0	T	C	100.0%	114.4 / NA	33	T410A (ACT→GCT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/16);  total (17/16)

GCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTC  >  NC_000913/1143025‑1143275                                                                                                                    |                                                                                                                                        gCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtg                                                                                                                 <  1:203980/140‑1 (MQ=255) gCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtg                                                                                                                 >  2:203980/1‑140 (MQ=255)    aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGaacaac                                                                                                                              >  1:234647/1‑124 (MQ=255)    aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGaacaac                                                                                                                              <  2:234647/124‑1 (MQ=255)    aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatg                                                                                                              >  2:749309/1‑140 (MQ=255)     ttGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatga                                                                                                             <  2:769724/140‑1 (MQ=255)      tGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGAc                                                                                                            <  2:340955/140‑1 (MQ=255)        gCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTg                                                                                                          <  1:683753/140‑1 (MQ=255)          acaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGa                                                                                                        <  2:267167/140‑1 (MQ=255)           caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                       <  1:518808/140‑1 (MQ=255)                                      tgtTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGa                                                                            <  1:749309/140‑1 (MQ=255)                                                     cttcttcttCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagc                                                             >  2:189618/1‑140 (MQ=255)                                                                aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCg                                                  >  2:99562/1‑140 (MQ=255)                                                                          gAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAATGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                        <  2:699914/140‑1 (MQ=255)                                                                           aaTAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGc                                       >  1:196369/1‑140 (MQ=255)                                                                           aaTAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGc                                       >  2:265299/1‑140 (MQ=255)                                                                               gagagCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAt                                   >  2:112534/1‑140 (MQ=255)                                                                                   gCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGa                               >  2:247231/1‑140 (MQ=255)                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                        <  1:401423/129‑1 (MQ=255)                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                        >  2:401423/1‑129 (MQ=255)                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAt                                   <  1:77755/134‑1 (MQ=255)                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAt                                   >  2:77755/1‑134 (MQ=255)                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAAt                             >  2:185635/1‑140 (MQ=255)                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAAt                             >  2:534410/1‑140 (MQ=255)                                                                                          cGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcgcg                        >  2:433764/1‑140 (MQ=255)                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      <  1:112534/140‑1 (MQ=255)                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      <  1:703887/140‑1 (MQ=255)                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      <  2:205327/140‑1 (MQ=255)                                                                                                       cgcacgGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACg           <  2:196369/140‑1 (MQ=255)                                                                                                         cacgGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTTCTGACGCa         <  1:99562/140‑1 (MQ=255)                                                                                                            ggTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCg      >  1:424566/1‑140 (MQ=255)                                                                                                              tGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCt    >  1:709847/1‑140 (MQ=255)                                                                                                                ccAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTc  >  1:511315/1‑140 (MQ=255)                                                                                                                    |                                                                                                                                        GCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTC  >  NC_000913/1143025‑1143275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑