| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,184,398 | G→A | P16L (CCC→CTC) | potB ← | spermidine preferential ABC transporter membrane subunit PotB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,184,398 | 0 | G | A | 97.4% | 125.2 / ‑4.2 | 38 | P16L (CCC→CTC) | potB | spermidine preferential ABC transporter membrane subunit PotB |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (17/20); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATATTCAGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAGCCAGTACGACCTCCCAGCTTTCTACCCAATTAATGGCCATTTTTTGGTCGAGAGAG > NC_000913/1184263‑1184533 | atatTCAGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAg < 1:123272/140‑1 (MQ=255) ttCAGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGaaa > 2:207876/1‑140 (MQ=255) cAGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGa < 1:207876/140‑1 (MQ=255) aGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGac > 2:800040/1‑140 (MQ=255) aGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGac > 1:28087/1‑140 (MQ=255) aGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGac > 2:423198/1‑140 (MQ=255) cGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGacaa < 1:618984/140‑1 (MQ=255) gAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGacaaa < 1:34729/140‑1 (MQ=255) tGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGacaaacaa > 2:67158/1‑140 (MQ=255) tGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGacaaacaa > 1:604885/1‑140 (MQ=255) cAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAacaaca > 1:578634/1‑140 (MQ=255) aCTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCa < 2:109518/140‑1 (MQ=255) tCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAAcc < 2:628644/140‑1 (MQ=255) cAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCg < 1:67158/140‑1 (MQ=255) cAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCg > 2:665466/1‑140 (MQ=255) agagCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATa < 2:444675/140‑1 (MQ=255) gCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTg < 2:578634/140‑1 (MQ=255) cGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTg > 2:19960/1‑139 (MQ=255) cGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTg < 1:19960/139‑1 (MQ=255) gATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACaa < 1:774783/132‑1 (MQ=255) gATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACaa > 2:774783/1‑132 (MQ=255) cAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTa < 2:458931/140‑1 (MQ=255) cAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTa < 2:28087/140‑1 (MQ=255) gACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACa < 2:604885/140‑1 (MQ=255) gcgTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTc < 2:306862/140‑1 (MQ=255) cGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTg > 2:379347/1‑126 (MQ=255) cGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTg < 1:379347/126‑1 (MQ=255) cGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGaa > 1:577135/1‑129 (MQ=255) cGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGaa < 2:577135/129‑1 (MQ=255) cGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTgtgt < 1:800040/140‑1 (MQ=255) aGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACAC‑AGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTtca > 1:42917/1‑140 (MQ=255) aTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAg < 1:665466/140‑1 (MQ=255) tttGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACTCAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAGcc > 2:266646/1‑140 (MQ=255) aaCTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACAC‑AGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAGCCAGTACGAcc < 2:42917/140‑1 (MQ=255) cgtcgtCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAGCCAGTACGACCTCCCa > 1:499718/1‑140 (MQ=255) cgtcgtCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAGCCAGTACGACCTCCCa < 1:423198/140‑1 (MQ=255) tcatcaGGTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAGCCAGTACGACCTCCCAGCTTTCTACCCAATTAATGGCCATTTTTTGGTc > 2:285529/1‑140 (MQ=255) gTTGAGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAGCCAGTACGACCTCCCAGCTTTCTACCCAATTAATGGCCATTTTTTGGTCGagagag > 1:366153/1‑140 (MQ=255) | ATATTCAGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGACAAACAACACAAGCCAACCGACAATAGTGACAATCACTACATTCTGGAACTTACTTGTGTTCTTCATCAGCCAGTACGACCTCCCAGCTTTCTACCCAATTAATGGCCATTTTTTGGTCGAGAGAG > NC_000913/1184263‑1184533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |