Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	59,087	T→C	L205P (CTA→CCA)	yabP →	putative uncharacterized protein YabP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	59,087	0	T	C	100.0%	135.2 / NA	39	L205P (CTA→CCA)	yabP	putative uncharacterized protein YabP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/20);  total (19/20)

CGGCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTA  >  NC_000913/58956‑59223                                                                                                                                    |                                                                                                                                         cGGCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACcccggacggtgccaacccgga                                                                                                                                  <  1:145912/140‑1 (MQ=255) cGGCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACcccggacggtgccaacccgga                                                                                                                                  <  1:707410/140‑1 (MQ=255)        gACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACcccggacggtgccaacccggacggtgct                                                                                                                           >  2:316520/1‑140 (MQ=255)        gACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACcccggacggtgccaacccggacggtgct                                                                                                                           >  2:797184/1‑140 (MQ=255)        gACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGG‑ATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACcccggacggtgccaacccggacggtgcta                                                                                                                          >  2:181487/1‑140 (MQ=255)              aGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTg                                                                                                                     >  1:100572/1‑140 (MQ=255)                ggATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGa                                                                                                                   <  2:262064/140‑1 (MQ=255)                ggATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGa                                                                                                                   <  2:689374/140‑1 (MQ=255)                  aTTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCAGACGGTGCCAACCCGGACGGTGCTAACCTGGACg                                                                                                                 >  2:315951/1‑140 (MQ=255)                    tGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGa                                                                                                                   >  1:544503/1‑136 (MQ=255)                    tGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGa                                                                                                                   <  2:544503/136‑1 (MQ=255)                    tGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGt                                                                                                               >  2:468885/1‑140 (MQ=255)                     gTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTg                                                                                                              >  2:45951/1‑140 (MQ=255)                         ccTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGG‑ATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTAcc                                                                                                         <  1:181487/140‑1 (MQ=255)                          cTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGa                                                                                                                   >  2:268787/1‑130 (MQ=255)                          cTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGa                                                                                                                   <  1:268787/130‑1 (MQ=255)                           ttGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCg                                                                                                        >  2:732653/1‑140 (MQ=255)                            tGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTg                                                                                                              >  2:277835/1‑133 (MQ=255)                            tGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTg                                                                                                              <  1:277835/133‑1 (MQ=255)                              gTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGa                                                                                                     <  2:100572/140‑1 (MQ=255)                                      aCAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCt                                                                                             <  1:161462/140‑1 (MQ=255)                                       cAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGTCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTa                                                                                            <  2:414421/140‑1 (MQ=255)                                       cAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTa                                                                                            <  1:141527/140‑1 (MQ=255)                                       cAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTa                                                                                            <  2:630496/140‑1 (MQ=255)                                       cAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTa                                                                                            <  1:523129/140‑1 (MQ=255)                                        aaTGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCAGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTAc                                                                                           <  1:315951/140‑1 (MQ=255)                                         aTGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTAcc                                                                                          <  2:447534/140‑1 (MQ=255)                                                    cAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCt                                                                                             <  2:432687/126‑1 (MQ=255)                                                    cAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCt                                                                                             >  1:432687/1‑126 (MQ=255)                                                             ccTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAat                                                                      >  2:85996/1‑140 (MQ=255)                                                                 tATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAattatt                                                                  >  1:333573/1‑140 (MQ=255)                                                                  aTGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAattatta                                                                 <  1:453773/140‑1 (MQ=255)                                                                   tGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAattattat                                                                <  1:180321/140‑1 (MQ=255)                                                                   tGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAattattat                                                                >  2:281924/1‑140 (MQ=255)                                                                        gaCCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATtaata                                                           >  1:57588/1‑140 (MQ=255)                                                                        gaCCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATtaata                                                           >  2:541255/1‑140 (MQ=255)                                                                              ggTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATtaataa                                                          >  2:717916/1‑135 (MQ=255)                                                                              ggTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATtaataa                                                          <  1:717916/135‑1 (MQ=255)                                                                                        tAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCaaaaa                                           >  2:323352/1‑140 (MQ=255)                                                                                              gggCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGa                                     <  2:333573/140‑1 (MQ=255)                                                                                              gggCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGa                                     <  1:468885/140‑1 (MQ=255)                                                                                               ggCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGAt                                    >  1:78972/1‑140 (MQ=255)                                                                                               ggCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGAGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGAt                                    >  2:120865/1‑140 (MQ=255)                                                                                                 cGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCCAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATAc                                  >  2:509437/1‑140 (MQ=255)                                                                                                                  tgctaccccggacggtgccaACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTg                 >  1:302833/1‑140 (MQ=255)                                                                                                                    ctaccccggacggtgccaACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTgaa               <  1:541255/140‑1 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGc                      >  2:292038/1‑125 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGc                      <  1:292038/125‑1 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTa                    >  1:533039/1‑127 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTa                    <  2:533039/127‑1 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTgaa               >  1:294690/1‑132 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTgaa               <  2:294690/132‑1 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGc       >  2:542697/1‑140 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGc       >  2:572132/1‑140 (MQ=255)                                                                                                                            gacggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGc       >  2:211642/1‑140 (MQ=255)                                                                                                                             acggtgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGct      <  1:85996/140‑1 (MQ=255)                                                                                                                                 tgccaacccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGctacta  >  2:185133/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         CGGCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTA  >  NC_000913/58956‑59223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑