Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,223,948	A→G	intergenic (+41/+331)	ycgI → / ← minE	protein YcgI/Z‑ring positioning protein MinE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,223,948	0	A	G	100.0%	184.4 / NA	53	intergenic (+41/+331)	ycgI/minE	protein YcgI/Z‑ring positioning protein MinE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/30);  total (23/30)

TACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTA  >  NC_000913/1223814‑1224063                                                                                                                                       |                                                                                                                      tACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGa                                                                                                                  <  2:231289/140‑1 (MQ=255)      ttAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTg                                                                                                             >  1:754397/1‑140 (MQ=255)        aGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGaa                                                                                                           <  2:13346/140‑1 (MQ=255)        aGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGaa                                                                                                           <  1:462527/140‑1 (MQ=255)           ggACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAgg                                                                                                        >  2:565515/1‑140 (MQ=255)                       gtggggtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTggg                                                                                            >  1:480348/1‑140 (MQ=255)                         ggggtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGc                                                                                          >  2:1031/1‑140 (MQ=255)                           ggtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCaa                                                                                        >  1:521713/1‑140 (MQ=255)                           ggtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTAGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAAGGAAAATTCTGGGGCaa                                                                                        >  2:716180/1‑140 (MQ=255)                            gtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCaaa                                                                                       >  1:588205/1‑140 (MQ=255)                            gtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCaaa                                                                                       >  1:18918/1‑140 (MQ=255)                                     tGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCg                                                                              >  2:713668/1‑140 (MQ=255)                                     tGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCg                                                                              >  1:653482/1‑140 (MQ=255)                                      ggTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCACGc                                                                             >  1:83562/1‑140 (MQ=255)                                       gTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGcc                                                                            >  2:511834/1‑140 (MQ=255)                                         gCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAg                                                                          >  1:431516/1‑140 (MQ=255)                                          ccGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGt                                                                         >  1:423439/1‑140 (MQ=255)                                          ccGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGt                                                                         >  2:115312/1‑140 (MQ=255)                                            ggTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTa                                                                       >  1:784221/1‑140 (MQ=255)                                             gtgtGGAATCCCGGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTAc                                                                      <  1:164212/140‑1 (MQ=255)                                             gtgtGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTAc                                                                      <  1:565515/140‑1 (MQ=255)                                                  gAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTAcggc‑gg                                                                >  2:199237/1‑140 (MQ=255)                                                   aaTCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑ggg                                                               <  2:588205/140‑1 (MQ=255)                                                   aaTCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCACGCCAGTTACGGC‑ggg                                                               <  2:83562/140‑1 (MQ=255)                                                       cccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGaaaa                                                           >  2:365636/1‑139 (MQ=255)                                                         cGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCt                                                        <  2:389828/140‑1 (MQ=255)                                                         cGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCt                                                        <  1:115312/140‑1 (MQ=255)                                                         cGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCt                                                        <  2:784221/140‑1 (MQ=255)                                                         cGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCt                                                        <  2:754397/140‑1 (MQ=255)                                                         cGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCt                                                        <  1:716180/140‑1 (MQ=255)                                                         cGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCt                                                        <  2:21190/140‑1 (MQ=255)                                                           tGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCa                                                      <  1:231772/140‑1 (MQ=255)                                                                     tGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGga                                            <  2:765984/140‑1 (MQ=255)                                                                                     gTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGGAAA‑CTCCATCCTTTGGG‑GAAAAAATAATAATGa                            >  2:169478/1‑140 (MQ=255)                                                                                     gTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑ACCCATCCTATTGGGGAAAAAAGaaaaaaaa                            >  2:371414/1‑137 (MQ=255)                                                                                          tCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTg                       <  1:321698/140‑1 (MQ=255)                                                                                             gACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGaa                                  <  2:636728/126‑1 (MQ=255)                                                                                             gACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGaa                                  >  1:636728/1‑126 (MQ=255)                                                                                             gACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTG‑AAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGGAA‑ACCCAACCTACGGGAGAACAAAGAAAAAAGAAAATGCggg                   >  2:12092/1‑140 (MQ=255)                                                                                                cATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGt                 <  2:716299/140‑1 (MQ=255)                                                                                                cATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGt                 <  1:242326/140‑1 (MQ=255)                                                                                                     accaTTAAACTGCCCTTCGGGGGAGTTTTTGTTTGCAAGATGTTGAAAGGGAAAATTTTGGGGCAAAAAAAACCCCGCAGGTTCCGC‑GGGAAACCCTAATCCAAGGGGA‑AACCATGGATAAATAAAATTCCGGGTTatca            <  2:423439/137‑1 (MQ=255)                                                                                                        aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCAtct         <  1:544541/140‑1 (MQ=255)                                                                                                        aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCAtct         <  2:521713/140‑1 (MQ=255)                                                                                                        aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCAtct         <  2:564279/140‑1 (MQ=255)                                                                                                        aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCCTTGGGGGGACAAAGAAAAATAAAAATTCGGGGGTATTAtct         >  2:402510/1‑140 (MQ=255)                                                                                                         ttAAACTGTGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCAtctc        <  2:347073/140‑1 (MQ=255)                                                                                                         ttAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACATTGAATAATGAAATTGCGGGGTTATCAtctc        <  2:211333/140‑1 (MQ=255)                                                                                                         ttAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCAtctc        <  2:18918/140‑1 (MQ=255)                                                                                                          tAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTcc       <  1:371414/140‑1 (MQ=255)                                                                                                          tAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTcc       <  1:450167/140‑1 (MQ=255)                                                                                                          tAAACTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTG‑AAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTccc      <  1:12092/140‑1 (MQ=255)                                                                                                            aaCTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGGGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCa     <  1:402510/140‑1 (MQ=255)                                                                                                             aCTGCGCTTCGGCGCAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg    <  1:169478/140‑1 (MQ=255)                                                                                                               tGCGCTTCGGCGAAGTTTTCGTTTGCAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGta  <  1:661406/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                      TACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGC‑GGGAAA‑CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTA  >  NC_000913/1223814‑1224063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑