Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,248,684	A→G	I144T (ATC→ACC)	dhaM ←	dihydroxyacetone kinase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,248,684	0	A	G	100.0%	132.4 / NA	38	I144T (ATC→ACC)	dhaM	dihydroxyacetone kinase subunit M
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

CAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGA  >  NC_000913/1248548‑1248813                                                                                                                                         |                                                                                                                                  cAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGCGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGttt                                                                                                                                <  2:715384/140‑1 (MQ=255)       cATATCGACATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAgtgt                                                                                                                          <  2:295965/140‑1 (MQ=255)            cGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCggag                                                                                                                     <  2:690857/140‑1 (MQ=255)            cGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCggag                                                                                                                     >  2:587303/1‑140 (MQ=255)            cGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCggag                                                                                                                     <  1:587303/140‑1 (MQ=255)                 ttAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACgg                                                                                                                >  2:641558/1‑140 (MQ=255)                  tAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGt                                                                                                               <  1:641558/140‑1 (MQ=255)                        tCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAAccc                                                                                                         >  2:511911/1‑140 (MQ=255)                          gATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAg                                                                                                       <  1:511911/140‑1 (MQ=255)                                  ataAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCAc                                                                                               <  1:190499/140‑1 (MQ=255)                                           ccGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCtt                                                                                      >  1:111083/1‑140 (MQ=255)                                           ccGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCtt                                                                                      <  1:280059/140‑1 (MQ=255)                                           ccGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCtt                                                                                      <  1:442221/140‑1 (MQ=255)                                             gggAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTcc                                                                                    >  2:98036/1‑140 (MQ=255)                                                             aTGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGt                                                                    <  1:420616/140‑1 (MQ=255)                                                                   gCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGt                                                                    >  1:219353/1‑134 (MQ=255)                                                                   gCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGt                                                                    <  2:219353/134‑1 (MQ=255)                                                                   gCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGa                                                              >  2:437977/1‑140 (MQ=255)                                                                   gCCGTTACGGTTTTTTATGACCACCGCAAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGa                                                              >  2:653216/1‑140 (MQ=255)                                                                        tACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt                                                         >  1:347694/1‑140 (MQ=255)                                                                                    tGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATcc                                             <  2:111083/140‑1 (MQ=255)                                                                                    tGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATcc                                             >  1:661049/1‑140 (MQ=255)                                                                                          ccGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccccc                                       >  1:93890/1‑140 (MQ=255)                                                                                           cGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCg                                      >  2:124449/1‑140 (MQ=255)                                                                                                     cGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCt                            >  1:393426/1‑140 (MQ=255)                                                                                                           tcttcATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGt                      >  1:323570/1‑140 (MQ=255)                                                                                                           tcttcATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGt                      >  1:482814/1‑140 (MQ=255)                                                                                                               cATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAcc                        <  2:352447/134‑1 (MQ=255)                                                                                                               cATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAcc                        >  1:352447/1‑134 (MQ=255)                                                                                                               cATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgtt                  <  2:482814/140‑3 (MQ=255)                                                                                                               cATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgct                  <  1:98036/140‑1 (MQ=255)                                                                                                                 tCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgctgc                <  1:763635/140‑1 (MQ=255)                                                                                                                   gTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTTACCGTtgctgc                >  2:632965/1‑138 (MQ=255)                                                                                                                   gTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTTACCGTtgctgc                <  1:632965/138‑1 (MQ=255)                                                                                                                      cGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGa                          <  2:588706/125‑1 (MQ=255)                                                                                                                      cGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGa                          >  1:588706/1‑125 (MQ=255)                                                                                                                            aCATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCtt     <  2:661049/140‑1 (MQ=255)                                                                                                                               tgtgTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGa  >  2:372016/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  CAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGA  >  NC_000913/1248548‑1248813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑