Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,371,377	A→G	M388V (ATG→GTG)	ycjM →	glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,371,377	0	A	G	100.0%	175.8 / NA	50	M388V (ATG→GTG)	ycjM	glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/26);  total (24/26)

ACCCGCTACGGGGCTTGTTGCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGA  >  NC_000913/1371243‑1371510                                                                                                                                       |                                                                                                                                      aCCCGCTACGGGGCTTGTTGCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGAt                                                                                                                                  >  2:14120/1‑140 (MQ=255)      cTACGGGGCTTGTTGCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGtt                                                                                                                             >  2:283474/1‑140 (MQ=255)          ggggCTTGTTGCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAgc                                                                                                                         <  1:435778/140‑1 (MQ=255)            ggCTTGTTGCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAgccg                                                                                                                       >  1:482716/1‑140 (MQ=255)            ggCTTGTTGCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGAGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAgccg                                                                                                                       >  2:108557/1‑140 (MQ=255)                 gttgCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTg                                                                                                                  >  2:413672/1‑140 (MQ=255)                        gAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAg                                                                                                           <  2:110515/140‑1 (MQ=255)                          aaGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCg                                                                                                         <  1:355222/140‑1 (MQ=255)                                      agagCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGtt                                                                                             <  1:690705/140‑1 (MQ=255)                                         gCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTgcg                                                                                          <  2:618955/140‑1 (MQ=255)                                          cTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTgcgc                                                                                         <  2:470369/140‑1 (MQ=255)                                          cTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTgcgc                                                                                         >  1:48025/1‑140 (MQ=255)                                          cTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTgcgc                                                                                         <  1:668785/140‑1 (MQ=255)                                          cTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTgcgc                                                                                         <  2:777720/140‑1 (MQ=255)                                           tGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACGGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGcc                                                                                        <  2:406666/140‑1 (MQ=255)                                             gTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAg                                                                                      >  1:419878/1‑140 (MQ=255)                                             gTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAg                                                                                      >  1:673199/1‑140 (MQ=255)                                               cGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGt                                                                                    <  2:584257/140‑1 (MQ=255)                                               cGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGt                                                                                    <  2:629261/140‑1 (MQ=255)                                                    cGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATc                                                                               <  2:419878/140‑1 (MQ=255)                                                            cagGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCa                                                                       >  2:444205/1‑140 (MQ=255)                                                                    tGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGAttt                                                               >  1:155626/1‑140 (MQ=255)                                                                      cATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCAATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTg                                                             >  2:766197/1‑140 (MQ=255)                                                                         tAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTa                                                          <  2:482716/140‑1 (MQ=255)                                                                              aaCTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGttt                                                     >  2:263566/1‑140 (MQ=255)                                                                              aaCTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGttt                                                     >  2:490131/1‑140 (MQ=255)                                                                               aCTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCAATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTc                                                    <  1:766197/140‑1 (MQ=255)                                                                                cTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTcc                                                   >  2:376235/1‑140 (MQ=255)                                                                                   gAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTcccct                                                <  1:283474/140‑2 (MQ=255)                                                                                   gAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCg                                                <  1:490131/140‑1 (MQ=255)                                                                                       aataatCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGgtgt                                            <  2:155626/140‑1 (MQ=255)                                                                                       aataatCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGgtgt                                            <  1:263566/140‑1 (MQ=255)                                                                                             cccGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGc                                      <  2:673199/140‑1 (MQ=255)                                                                                                 aCGGTACACGCAGTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGata                                  <  1:14120/140‑1 (MQ=255)                                                                                                             gTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGt                      >  2:477064/1‑140 (MQ=255)                                                                                                             gTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGt                      >  2:707174/1‑140 (MQ=255)                                                                                                             gTCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGt                      >  1:678703/1‑140 (MQ=255)                                                                                                              tCCGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTa                     >  1:42935/1‑140 (MQ=255)                                                                                                               ccGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTAt                    >  1:506405/1‑140 (MQ=255)                                                                                                               ccGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTAt                    >  2:731224/1‑140 (MQ=255)                                                                                                               ccGTATGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTAt                    >  2:140618/1‑140 (MQ=255)                                                                                                                   aTGAAATAAATGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCtt                >  1:80501/1‑140 (MQ=255)                                                                                                                       aataaatGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCt            <  2:48025/140‑1 (MQ=255)                                                                                                                       aataaatGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCt            <  1:444205/140‑1 (MQ=255)                                                                                                                       aataaatGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCt            <  2:80501/140‑1 (MQ=255)                                                                                                                            atGTGACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGt       <  2:678703/140‑1 (MQ=255)                                                                                                                               tgACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAAt    <  1:477064/140‑1 (MQ=255)                                                                                                                                gACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGt       >  1:211105/1‑136 (MQ=255)                                                                                                                                gACCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGt       <  2:211105/136‑1 (MQ=255)                                                                                                                                 aCCTATGTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGa  >  1:471613/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      ACCCGCTACGGGGCTTGTTGCCTGAAAGCGAAATATTAGAGCTGGTCGAGGCGTTACAGCAGGAAGGTGCATTAGTAAACTGGAAAAATAATCCCGACGGTACACGCAGTCCGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGA  >  NC_000913/1371243‑1371510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑