Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,398,338 A→G R95R (CGT→CGC)  uspE ← universal stress protein with a role cellular motility

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,398,3380AG100.0% 175.6 / NA 50R95R (CGT→CGC) uspEuniversal stress protein with a role cellular motility
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/24);  total (26/24)

GGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCGGGGAGAGCAG  >  NC_000913/1398202‑1398472
                                                                                                                                        |                                                                                                                                      
ggCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCgg                                                                                                                                     <  2:7271/140‑1 (MQ=255)
ggCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCgg                                                                                                                                     >  1:7271/1‑140 (MQ=255)
 gCTTGGGCATTTGCTTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCgg                                                                                                                                     >  2:347590/1‑139 (MQ=255)
 gCTTGGGCATTTGCTTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCgg                                                                                                                                     <  1:347590/139‑1 (MQ=255)
 gCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGt                                                                                                                                    >  2:377727/1‑140 (MQ=255)
      ggCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGc                                                                                                                               >  2:726428/1‑140 (MQ=255)
                      aTGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAAttt                                                                                                               >  1:713192/1‑140 (MQ=255)
                       tGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGAc                                                                                                                          <  2:103170/128‑1 (MQ=255)
                       tGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGAc                                                                                                                          >  1:103170/1‑128 (MQ=255)
                        gCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCa                                                                                                             >  2:555366/1‑140 (MQ=255)
                              ccGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGa                                                                                                       >  1:365922/1‑140 (MQ=255)
                                  tGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGc                                                                                                   <  2:365922/140‑1 (MQ=255)
                                  tGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGc                                                                                                   <  1:658065/140‑1 (MQ=255)
                                  tGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGc                                                                                                   >  1:717899/1‑140 (MQ=255)
                                    gCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAGTGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCa                                                                                                 >  1:24217/1‑140 (MQ=255)
                                       tAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCa                                                                                              <  1:275915/140‑1 (MQ=255)
                                        aaaaaTCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCAt                                                                                             <  1:247135/140‑1 (MQ=255)
                                        aaaaaTCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCAt                                                                                             <  1:726428/140‑1 (MQ=255)
                                          aaaTCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTg                                                                                           <  2:476230/140‑1 (MQ=255)
                                                          aCGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCt                                                                           <  2:365681/140‑1 (MQ=255)
                                                               cATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCg                                                                      <  1:239327/140‑1 (MQ=255)
                                                                 tGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTg                                                                    >  2:260529/1‑140 (MQ=255)
                                                                     ggTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATc                                                                <  1:377727/140‑1 (MQ=255)
                                                                      gTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATcc                                                               <  1:260529/140‑1 (MQ=255)
                                                                      gTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATcc                                                               <  1:4326/140‑1 (MQ=255)
                                                                        gcgcCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAg                                                             >  1:699536/1‑140 (MQ=255)
                                                                        gcgcCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAg                                                             >  2:379902/1‑140 (MQ=255)
                                                                                     cACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACgctggc                                                >  2:242072/1‑140 (MQ=255)
                                                                                     cACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACgctggc                                                >  1:450086/1‑140 (MQ=255)
                                                                                               tgtCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGcc                                      >  2:465342/1‑140 (MQ=255)
                                                                                                 tCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCt                                    >  2:151455/1‑140 (MQ=255)
                                                                                                  ccgccgCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTg                                   <  2:713192/140‑1 (MQ=255)
                                                                                                  ccgccgCTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTg                                   <  1:382364/140‑1 (MQ=255)
                                                                                                      cgcTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGc                               >  2:351075/1‑140 (MQ=255)
                                                                                                        cTGATCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCAt                             <  1:530695/140‑1 (MQ=255)
                                                                                                            tCACTTCCTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCg                         <  2:259044/140‑1 (MQ=255)
                                                                                                                  ccTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGt                   >  2:67214/1‑140 (MQ=255)
                                                                                                                  ccTGAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGt                   >  1:151122/1‑140 (MQ=255)
                                                                                                                     gAATGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCg                >  2:185327/1‑140 (MQ=255)
                                                                                                                      aaTGATGGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGt               >  2:660989/1‑140 (MQ=255)
                                                                                                                        tgatgGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTcc             >  2:229013/1‑140 (MQ=255)
                                                                                                                          atgGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCgg           >  2:411659/1‑140 (MQ=255)
                                                                                                                          atgGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCgg           >  2:479469/1‑140 (MQ=255)
                                                                                                                          atgGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCgg           >  1:488951/1‑140 (MQ=255)
                                                                                                                          atgGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCgg           <  1:465342/140‑1 (MQ=255)
                                                                                                                           tgGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCggg          <  1:411659/140‑1 (MQ=255)
                                                                                                                           tgGCTTCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCggg          <  1:379902/140‑1 (MQ=255)
                                                                                                                               ttCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCGGGgaga      <  2:450086/140‑1 (MQ=255)
                                                                                                                                tCGAAAGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCGGGGagag     <  2:717899/140‑1 (MQ=255)
                                                                                                                                   aaaGGGCGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCGGGGAGAGCAg  <  1:653509/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCCTGAATGATGGCTTCGAAAGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCGGGGAGAGCAG  >  NC_000913/1398202‑1398472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑