Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,423,030	A→G	P93P (CCA→CCG)	ydaW →	Rac prophage; putative uncharacterized protein YdaW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,423,030	0	A	G	100.0%	182.8 / NA	52	P93P (CCA→CCG)	ydaW	Rac prophage; putative uncharacterized protein YdaW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/26);  total (26/26)

TACACCTGGCGTGTGGCTGATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCACAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGC  >  NC_000913/1422893‑1423165                                                                                                                                          |                                                                                                                                        tACACCTGGCGTGTGGCTGATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACcgca                                                                                                                                       >  2:412227/1‑140 (MQ=255)        ggCGTGTGGCTGATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCt                                                                                                                                >  1:56457/1‑140 (MQ=255)             gtgGCTGATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCtgatga                                                                                                                           <  1:434597/140‑1 (MQ=255)              tgGCTGATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAg                                                                                                                          >  1:22092/1‑140 (MQ=255)               ggCTGATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGc                                                                                                                         <  1:193209/140‑1 (MQ=255)                gCTGATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCt                                                                                                                        >  2:240043/1‑140 (MQ=255)                   gATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAg                                                                                                                          >  1:24036/1‑135 (MQ=255)                   gATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAg                                                                                                                          <  2:24036/135‑1 (MQ=255)                       aCAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACa                                                                                                                 <  1:737191/140‑1 (MQ=255)                          aTGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTg                                                                                                              <  2:777000/140‑1 (MQ=255)                           tGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTa                                                                                                                   <  1:759103/134‑1 (MQ=255)                           tGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTa                                                                                                                   >  2:759103/1‑134 (MQ=255)                           tGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGt                                                                                                             >  1:223942/1‑140 (MQ=255)                            gTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGtt                                                                                                            <  2:22092/140‑1 (MQ=255)                              tCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTa                                                                                                                   >  2:108744/1‑131 (MQ=255)                              tCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTa                                                                                                                   <  1:108744/131‑1 (MQ=255)                                agcagGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACa                                                                                                                 <  1:427491/131‑1 (MQ=255)                                agcagGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACa                                                                                                                 >  2:427491/1‑131 (MQ=255)                                 gcagGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTa                                                                                                       <  2:223942/140‑1 (MQ=255)                                  cagGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGtt                                                                                                            >  2:765996/1‑134 (MQ=255)                                  cagGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGtt                                                                                                            <  1:765996/134‑1 (MQ=255)                                  cagGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGtgc                                                                                                      >  2:742987/1‑138 (MQ=255)                                     gAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGtt                                                                                                            >  2:114640/1‑131 (MQ=255)                                     gAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGtt                                                                                                            <  1:114640/131‑1 (MQ=255)                                       aCAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACAt                                                                                                 >  1:4611/1‑140 (MQ=255)                                       aCAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACAt                                                                                                 >  1:279870/1‑140 (MQ=255)                                            cagcGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGc                                                                                            >  1:339352/1‑140 (MQ=255)                                            cagcGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGc                                                                                            <  2:279870/140‑1 (MQ=255)                                               cgcAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCaaa                                                                                         >  2:633712/1‑140 (MQ=255)                                                      agAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGGGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCtt                                                                                  <  2:3505/140‑1 (MQ=255)                                                                    aaaTCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCtggcaatg                                                                    <  2:4611/140‑1 (MQ=255)                                                                              aGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAGCGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCa                                                          >  2:237719/1‑140 (MQ=255)                                                                                     gTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGgtcg                                                   >  2:562638/1‑140 (MQ=255)                                                                                           aaaaTCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGAt                                             <  2:528823/140‑1 (MQ=255)                                                                                              aTCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTcg                                          >  2:160970/1‑140 (MQ=255)                                                                                                       tGCGATTTAACCGCGACGATTGAACAACGAGGATCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCa                                 >  1:780465/1‑140 (MQ=255)                                                                                                                 ccGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTaaa                       >  2:119143/1‑140 (MQ=255)                                                                                                                 ccGCGACGATTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTaaa                       >  1:553496/1‑140 (MQ=255)                                                                                                                    cgacgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAAttt                    <  1:633712/140‑1 (MQ=255)                                                                                                                    cgacgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAAttt                    >  1:519027/1‑140 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGATCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCg                  <  2:780465/140‑1 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAgg                               <  2:296918/127‑1 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAgg                               >  1:296918/1‑127 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCg                  <  2:339352/140‑1 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCg                  >  2:264220/1‑140 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCg                  <  2:56457/140‑1 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCg                  <  1:562638/140‑1 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCg                  <  1:412227/140‑1 (MQ=255)                                                                                                                      acgaTTGAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCg                  <  1:160970/140‑1 (MQ=255)                                                                                                                            gAACAACGAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTg            >  2:470594/1‑140 (MQ=255)                                                                                                                                   gAGGACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCg     >  1:625919/1‑140 (MQ=255)                                                                                                                                      gACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGc  <  2:113203/140‑1 (MQ=255)                                                                                                                                      gACCGCAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGc  <  1:240043/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TACACCTGGCGTGTGGCTGATAACAATGTTCAGCAGGAACAGCCAGCGCAGGCAGAACTGCCGGAAGAAATCACCACAGCAACAGTAGCGAAAATCTCAGAGTGCGATTTAACCGCGACGATTGAACAACGAGGACCACAAACGGCTGATGAGCTGGCTACATTGTTTGGTACCACATCACGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGGTCGTCTGATTCGCGTAAATCAGGGCGGTAAATTTCGTTACTGCATACCGGGC  >  NC_000913/1422893‑1423165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑