Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,483,525	T→C	G155G (GGT→GGC)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,483,525	0	T	C	100.0%	190.1 / NA	54	G155G (GGT→GGC)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/30);  total (24/30)

TGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAA  >  NC_000913/1483400‑1483654                                                                                                                              |                                                                                                                                  tGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGttt                                                                                                                     <  2:471873/140‑1 (MQ=255)        aGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGAt                                                                                                              >  1:133261/1‑140 (MQ=255)        aGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGAt                                                                                                              >  2:625715/1‑140 (MQ=255)          cTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGa                                                                                                               >  2:485595/1‑137 (MQ=255)          cTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGa                                                                                                               <  1:485595/137‑1 (MQ=255)            ggggCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACg                                                                                                          <  1:71001/140‑1 (MQ=255)             gggCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGt                                                                                                         <  2:612134/140‑1 (MQ=255)             gggCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGt                                                                                                         <  2:550784/140‑1 (MQ=255)                       ttAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAac                                                                                               >  2:90378/1‑140 (MQ=255)                             gACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATg                                                                                         >  1:166404/1‑140 (MQ=255)                                tGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGAt                                                                                          >  1:389504/1‑136 (MQ=255)                                tGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGAt                                                                                          <  2:389504/136‑1 (MQ=255)                                 gATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCa                                                                                     >  1:626606/1‑140 (MQ=255)                                  aTCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCaa                                                                                    >  1:259322/1‑140 (MQ=255)                                  aTCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCaa                                                                                    >  1:467924/1‑140 (MQ=255)                                           aCCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGAc                                                                           <  1:625715/140‑1 (MQ=255)                                            cccAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATgg                                                                                        <  2:781698/126‑1 (MQ=255)                                            cccAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATgg                                                                                        >  1:781698/1‑126 (MQ=255)                                            cccAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGAcc                                                                          >  2:40984/1‑140 (MQ=255)                                              cAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGa                                                                        <  2:688582/140‑1 (MQ=255)                                                 ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGAc                                                                           >  1:371796/1‑134 (MQ=255)                                                 ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGAc                                                                           <  2:371796/134‑1 (MQ=255)                                                 ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACgg                                                                     >  1:717189/1‑140 (MQ=255)                                                 ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACgg                                                                     >  1:273099/1‑140 (MQ=255)                                                 ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACgg                                                                     >  2:439208/1‑140 (MQ=255)                                                 ccGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACgg                                                                     >  1:529243/1‑140 (MQ=255)                                                    cgtcgtCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTAt                                                                  >  2:335142/1‑140 (MQ=255)                                                    cgtcgtCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTAt                                                                  <  2:598263/140‑1 (MQ=255)                                                    cgtcgtCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTAt                                                                  >  1:298544/1‑140 (MQ=255)                                                      tcgtcTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATcc                                                                >  2:627323/1‑140 (MQ=255)                                                        gtcTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCctg                                                              <  1:40984/140‑1 (MQ=255)                                                              cggcAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTCTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCg                                                        <  2:717189/140‑1 (MQ=255)                                                               ggcAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCgg                                                       <  1:627323/140‑1 (MQ=255)                                                                  aaGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGTGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGgaga                                                    <  2:133261/140‑1 (MQ=255)                                                                            gCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGa                                          >  2:10702/1‑140 (MQ=255)                                                                               aaCCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCg                                       <  1:335142/140‑1 (MQ=255)                                                                               aaCCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCg                                       <  1:534411/140‑1 (MQ=255)                                                                                    tATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCctgc                                  <  2:467924/140‑1 (MQ=255)                                                                                       tGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGa                               <  2:166404/140‑1 (MQ=255)                                                                                       tGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGa                               <  2:259322/140‑1 (MQ=255)                                                                                         cGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATg                             <  1:302410/140‑1 (MQ=255)                                                                                         cGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATg                             >  2:302410/1‑140 (MQ=255)                                                                                            aaGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAg                          <  2:407250/140‑1 (MQ=255)                                                                                              gagCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTa                        >  1:28514/1‑140 (MQ=255)                                                                                              gagCTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTa                        <  2:28514/140‑1 (MQ=255)                                                                                                 cTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGa                     >  1:714978/1‑140 (MQ=255)                                                                                                 cTGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGa                     <  1:90378/140‑1 (MQ=255)                                                                                                  tGAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGac                    <  2:445708/140‑1 (MQ=255)                                                                                                   gAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGaca                   <  2:529243/140‑1 (MQ=255)                                                                                                   gAAAACGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGaca                   <  2:273099/140‑1 (MQ=255)                                                                                                      aaCGGAGCCGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTa                <  1:10702/140‑1 (MQ=255)                                                                                                             ccGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGaca                   <  1:282545/130‑1 (MQ=255)                                                                                                             ccGGGCGGTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGaca                   >  2:282545/1‑130 (MQ=255)                                                                                                                    gTTGCATCGGCTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGaa  <  1:439208/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                  TGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAA  >  NC_000913/1483400‑1483654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑