Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,169	A→G	A185A (GCA→GCG)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,169	0	A	G	100.0%	135.6 / NA	40	A185A (GCA→GCG)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

CGAATATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCACCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGAA  >  NC_000913/132034‑132300                                                                                                                                        |                                                                                                                                    cGAATATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCgg                                                                                                                                 >  1:549206/1‑140 (MQ=255)      atGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTTTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCg                                                                                                                            <  2:248234/140‑1 (MQ=255)       tGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGt                                                                                                                           <  1:128145/140‑1 (MQ=255)          gAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGt                                                                                                                        <  1:369921/140‑1 (MQ=255)               aGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGc                                                                                                                   <  2:288722/140‑1 (MQ=255)                 gTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGccc                                                                                                                 >  1:78364/1‑140 (MQ=255)                           ccTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCgg                                                                                                               >  1:87173/1‑132 (MQ=255)                           ccTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCgg                                                                                                               <  2:87173/132‑1 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                              >  2:787336/1‑140 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                              >  2:574909/1‑140 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                              >  2:394261/1‑140 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                              >  2:385441/1‑140 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                              >  2:38317/1‑140 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                              >  2:317965/1‑140 (MQ=255)                                        cGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTgg                                                                                          <  1:255721/140‑1 (MQ=255)                                        cGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTgg                                                                                          <  1:358793/140‑1 (MQ=255)                                        cGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTgg                                                                                          <  1:456324/140‑1 (MQ=255)                                                       tCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACg                                                                           <  2:351174/140‑1 (MQ=255)                                                        cGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGA‑CTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGcc                                                                         <  2:119010/140‑1 (MQ=255)                                                          cccGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGccc                                                                        >  1:230393/1‑140 (MQ=255)                                                              gcgcTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGA‑GCTGAAAAACGCCCGTGaa                                                                   <  1:174950/140‑1 (MQ=255)                                                                    gctgAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTAt                                                              <  2:230393/140‑1 (MQ=255)                                                                              tGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGAc                                                    <  1:385441/140‑1 (MQ=255)                                                                                      aCTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                            <  1:574909/140‑1 (MQ=255)                                                                                      aCTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                            <  1:38317/140‑1 (MQ=255)                                                                                      aCTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                            <  2:78364/140‑1 (MQ=255)                                                                                       cTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGAc                                                    >  1:761812/1‑131 (MQ=255)                                                                                       cTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGAc                                                    <  2:761812/131‑1 (MQ=255)                                                                                          tCTTCAAAGTCACTGTCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                            <  2:326209/136‑1 (MQ=255)                                                                                          tCTTCAAAGTCACTGTCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                            >  1:326209/1‑136 (MQ=255)                                                                                            ttCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGAc                                                    <  2:235528/126‑1 (MQ=255)                                                                                            ttCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGAc                                                    >  1:235528/1‑126 (MQ=255)                                                                                                    cACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCCTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGa                              >  2:549243/1‑140 (MQ=255)                                                                                                                   cacCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCTCTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAg               <  1:185658/140‑1 (MQ=255)                                                                                                                        aTGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCg                  >  1:280795/1‑132 (MQ=255)                                                                                                                        aTGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCg                  <  2:280795/132‑1 (MQ=255)                                                                                                                        aTGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTg          >  2:624988/1‑140 (MQ=255)                                                                                                                         tGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGc         >  2:686567/1‑140 (MQ=255)                                                                                                                               ttCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGa   >  1:347742/1‑140 (MQ=255)                                                                                                                                tctcCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGaa  <  2:382608/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CGAATATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCACCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGAA  >  NC_000913/132034‑132300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑