Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,365	T→C	V61A (GTG→GCG)	speG →	spermidine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,365	0	T	C	100.0%	201.1 / NA	57	V61A (GTG→GCG)	speG	spermidine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/22);  total (35/22)

TGTACATCAACTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGTGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGC  >  NC_000913/1656240‑1656494                                                                                                                              |                                                                                                                                  tGTACATCAACTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACg                                                                                                                     >  1:776868/1‑140 (MQ=255)      aTCAACTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGaa                                                                                                                >  2:471993/1‑140 (MQ=255)         aaCTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGaaaaa                                                                                                             >  2:275319/1‑140 (MQ=255)           cTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAGGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGc                                                                                                           >  1:482196/1‑140 (MQ=255)           cTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGaaaaaac                                                                                                           >  1:659441/1‑138 (MQ=255)           cTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGc                                                                                                           <  2:776868/140‑1 (MQ=255)           cTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGc                                                                                                           >  1:71796/1‑140 (MQ=255)           cTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAACATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGc                                                                                                           >  2:550790/1‑140 (MQ=255)             cGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCg                                                                                                         >  2:482901/1‑140 (MQ=255)             cGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCg                                                                                                         >  2:178406/1‑140 (MQ=255)               aCAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGt                                                                                                       <  1:482901/140‑1 (MQ=255)                           gtgtGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCtg                                                                                           >  1:446140/1‑140 (MQ=255)                             gtgATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCtggt                                                                                         >  2:709012/1‑140 (MQ=255)                                   cGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAg                                                                                              <  1:730426/129‑1 (MQ=255)                                   cGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAg                                                                                              >  2:730426/1‑129 (MQ=255)                                      tACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAg                                                                                              <  1:280989/126‑1 (MQ=255)                                      tACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAg                                                                                              >  2:280989/1‑126 (MQ=255)                                        cTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAAcc                                                                              <  1:275319/140‑1 (MQ=255)                                        cTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAAcc                                                                              <  1:709012/140‑1 (MQ=255)                                           gTTTTAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATg                                                                           >  2:527157/1‑140 (MQ=255)                                             ttGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGtt                                                                         >  2:470670/1‑140 (MQ=255)                                                aGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCAt                                                                      >  2:199345/1‑140 (MQ=255)                                                               ccTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAAttt                                                       >  1:649819/1‑140 (MQ=255)                                                                cttggttgAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTc                                                      >  2:11397/5‑140 (MQ=255)                                                                cTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTc                                                      <  1:178406/140‑1 (MQ=255)                                                                 tttGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCa                                                     <  1:471993/140‑1 (MQ=255)                                                                   tgttgAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGa                                                   <  2:180012/140‑1 (MQ=255)                                                                            ctctGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTAtct                                          <  1:614334/140‑1 (MQ=255)                                                                             tctGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTAtctc                                         >  1:755078/1‑140 (MQ=255)                                                                             tctGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTAtct                                          <  2:417290/139‑1 (MQ=255)                                                                             tctGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTAtct                                          >  1:417290/1‑139 (MQ=255)                                                                               tGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTccc                                       <  1:199345/140‑1 (MQ=255)                                                                                gATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTcccc                                      <  1:784483/140‑1 (MQ=255)                                                                                    tGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAg                                  <  2:71796/140‑1 (MQ=255)                                                                                      tATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTa                                >  1:667075/1‑140 (MQ=255)                                                                                       aTGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCgg                                    >  1:528302/1‑135 (MQ=255)                                                                                       aTGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCgg                                    <  2:528302/135‑1 (MQ=255)                                                                                         gATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCa                             >  2:269727/1‑140 (MQ=255)                                                                                           tAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTAt                                            <  1:592516/123‑1 (MQ=255)                                                                                           tAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTAt                                            >  2:592516/1‑123 (MQ=255)                                                                                           tAAGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAgg                           >  2:217812/1‑140 (MQ=255)                                                                                            aaGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAggg                          <  2:649819/140‑1 (MQ=255)                                                                                             aGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAgggg                         <  1:217812/140‑1 (MQ=255)                                                                                             aGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAgggg                         <  1:470670/140‑1 (MQ=255)                                                                                             aGCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAgggg                         >  1:590368/1‑140 (MQ=255)                                                                                              gCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCTGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGa                                   <  2:118705/129‑1 (MQ=255)                                                                                              gCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCTGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGa                                   >  1:118705/1‑129 (MQ=255)                                                                                              gCATATTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGa                        >  2:465419/1‑140 (MQ=255)                                                                                                atatTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGaaa                      >  2:488322/1‑140 (MQ=255)                                                                                                atatTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGaaa                      >  2:474954/1‑140 (MQ=255)                                                                                                 tatTCACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGAGAAAg                     <  2:424318/140‑1 (MQ=255)                                                                                                     cACGATCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCt                 >  1:523081/1‑140 (MQ=255)                                                                                                          tCAGAGCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCaa            >  1:632258/1‑140 (MQ=255)                                                                                                              agCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCg        >  1:146061/1‑140 (MQ=255)                                                                                                              agCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCg        >  1:173218/1‑140 (MQ=255)                                                                                                              agCGAACGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCg        >  1:354159/1‑140 (MQ=255)                                                                                                                    cGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTgccgc  <  1:432978/140‑1 (MQ=255)                                                                                                                    cGGCGCTTTGCGGTGGAATGTGACGGCGAAAAAGCCGGACTGGTGTAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGTAAGGACTGGCAACCCGTgccgc  >  2:486012/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                  TGTACATCAACTCGACAATAACGCCAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACGATCAGAGCGAACGGCGCTTTGTGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGC  >  NC_000913/1656240‑1656494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑