Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,008	T→C	G184G (GGT→GGC)	mdtK →	multidrug efflux pump MdtK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,008	0	T	C	100.0%	77.1 / NA	23	G184G (GGT→GGC)	mdtK	multidrug efflux pump MdtK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/12);  total (11/12)

CAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCAA  >  NC_000913/1743889‑1744141                                                                                                                        |                                                                                                                                       cAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCgt                                                                                                                    <  2:146362/140‑1 (MQ=255)      tGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCgttggtt                                                                                                               <  2:619081/140‑1 (MQ=255)                   tgtgAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGACATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTAc                                                                                                  >  2:431376/1‑140 (MQ=255)                         ggTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTgcggc                                                                                            <  1:581661/140‑1 (MQ=255)                          gTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTgcggcg                                                                                           <  2:310688/140‑1 (MQ=255)                          gTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTgcggcg                                                                                           >  1:122227/1‑140 (MQ=255)                              ggCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGgtgt                                                                                       <  1:464676/140‑1 (MQ=255)                               gCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTa                                                                                      <  1:2995/140‑1 (MQ=255)                                        aaGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCAt                                                                            <  1:689572/140‑1 (MQ=255)                                          gCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGt                                                                          >  1:116144/1‑140 (MQ=255)                                                  tGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGcc                                                                  >  1:280522/1‑140 (MQ=255)                                                   ggTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCa                                                                 <  1:128168/140‑1 (MQ=255)                                                     tAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCATg                                                               <  2:646386/140‑1 (MQ=255)                                                     tAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCATg                                                               <  1:210829/140‑1 (MQ=255)                                                            cTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCATGGGTTCtt                                                        >  1:261314/1‑140 (MQ=255)                                                                    gCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTAAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCATGGTTTCTTACATTaaa                                                <  1:743966/140‑1 (MQ=255)                                                                     ccTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGGGTA‑TTGGGTCATGTTCCTTGCCATGGGTTCTTACATTaaaa                                               >  2:677766/1‑139 (MQ=255)                                                                         ctgGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTAAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTTGGGCATGTTTCTTTCCATGGGTTTTTTCATTAAACgcgc                                           >  2:743966/1‑140 (MQ=255)                                                                                      cGGTGAACTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCATGGGTTCTTACATTAAACGCGCCCGCTCCATgcgc                              >  1:329486/1‑140 (MQ=255)                                                                                             cTATATCTTTATTTATGGTCATTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGGGTA‑TTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATAttt                       >  1:661987/1‑139 (MQ=255)                                                                                                                 aTTTCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCa   <  1:677766/140‑1 (MQ=255)                                                                                                                  tttCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGGGGTGGATTTGGGGCAT‑TTCCTTGACCTGGGTTTTTTTATTAAACGCGCCCGCTCCGTCCGCGACATTGCTCACTAAAAAAGCACCGcga  >  2:277665/1‑138 (MQ=255)                                                                                                                  tttCGGCATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTTGGTCATGTTCCTTGCCATGGTTTCTTTCATTAAACGCGCCCGCCCCCTGCGCGATATTCGGAACGAAAAAGGCACCGCaa  >  2:675690/1‑140 (MQ=255)                                                                                                                        |                                                                                                                                       CAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTA‑TTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCAA  >  NC_000913/1743889‑1744141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑