Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	207,792	C→T	D889D (GAC→GAT)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	207,792	0	C	T	100.0%	147.0 / NA	42	D889D (GAC→GAT)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/19);  total (23/19)

TCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACA  >  NC_000913/207657‑207923                                                                                                                                        |                                                                                                                                    tCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTc                                                                                                                                 <  1:99643/140‑1 (MQ=255)              atcatcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATcg                                                                                                                    >  2:318938/1‑140 (MQ=255)              atcatcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGCGGCGCTTGATCCTCTTCGGCCACATcc                                                                                                                    >  2:588508/1‑139 (MQ=255)                catcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATcgcg                                                                                                                  <  2:310075/140‑1 (MQ=255)                catcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATcgcg                                                                                                                  <  2:271400/140‑1 (MQ=255)                catcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATcgcg                                                                                                                  <  1:318938/140‑1 (MQ=255)                            taataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCtgatga                                                                                                      >  1:144531/1‑140 (MQ=255)                            taataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCtgatga                                                                                                      <  1:351442/140‑1 (MQ=255)                                     cggcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTgg                                                                                             >  2:484230/1‑140 (MQ=255)                                      ggcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTggg                                                                                            >  2:207456/1‑140 (MQ=255)                                       gcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGc                                                                                           >  2:306455/1‑140 (MQ=255)                                                     cTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGc                                                                             >  1:735013/1‑140 (MQ=255)                                                       gTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCgg                                                                           <  1:432550/140‑1 (MQ=255)                                                        tttGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGc                                                                          <  2:188972/140‑1 (MQ=255)                                                                ctGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAac                                                                  >  1:432928/1‑140 (MQ=255)                                                                ctGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAac                                                                  >  2:277504/1‑140 (MQ=255)                                                                ctGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAac                                                                  >  1:647563/1‑140 (MQ=255)                                                                 tGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAaca                                                                 <  2:647563/140‑1 (MQ=255)                                                                  gcgcCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAacac                                                                >  1:512422/1‑140 (MQ=255)                                                                        gTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGaaa                                                          >  1:105813/1‑140 (MQ=255)                                                                         tACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAg                                                         >  2:145641/1‑140 (MQ=255)                                                                           ccGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCg                                                       >  1:708508/1‑140 (MQ=255)                                                                             gACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGa                                                     <  2:238668/140‑1 (MQ=255)                                                                             gACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGa                                                     >  2:594301/1‑140 (MQ=255)                                                                                   aaaaaGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGaaa                                                          <  2:33559/129‑1 (MQ=255)                                                                                   aaaaaGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGaaa                                                          >  1:33559/1‑129 (MQ=255)                                                                                    aaaaGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAg                                                         <  2:471827/129‑1 (MQ=255)                                                                                    aaaaGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAg                                                         >  1:471827/1‑129 (MQ=255)                                                                                       aGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGt                                           <  1:145641/140‑1 (MQ=255)                                                                                        gTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTc                                          <  1:277504/140‑1 (MQ=255)                                                                                        gTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTc                                          <  1:594301/140‑1 (MQ=255)                                                                                                 tCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCCGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGata                                 >  1:767492/1‑140 (MQ=255)                                                                                                   gcgTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAgg                                       >  1:395379/1‑132 (MQ=255)                                                                                                   gcgTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAgg                                       <  2:395379/132‑1 (MQ=255)                                                                                                      tGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGt                                           <  2:145363/125‑1 (MQ=255)                                                                                                      tGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGt                                           >  1:145363/1‑125 (MQ=255)                                                                                                        cTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCgg                          >  2:195566/1‑140 (MQ=255)                                                                                                                   gATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGc                     >  2:74516/1‑134 (MQ=255)                                                                                                                   gATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGc                     <  1:74516/134‑1 (MQ=255)                                                                                                                     tCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGaa             >  1:577472/1‑140 (MQ=255)                                                                                                                      cATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAag            <  2:105813/140‑1 (MQ=255)                                                                                                                              gggCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGaa    >  2:637288/1‑140 (MQ=255)                                                                                                                                gCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACa  <  2:144531/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCTGGGCGATGCGTTAAAAGCGGCAGATCAACACGCGAAAGCGGAAGCTATCGGTCAGGCCGATATGTTCGGCGTGCTGGCCGAAGAGCCGGAACA  >  NC_000913/207657‑207923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑