Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,893,650	T→C	V95A (GTA→GCA)	yoaB →	RutC family protein YoaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,893,650	0	T	C	100.0%	85.6 / NA	26	V95A (GTA→GCA)	yoaB	RutC family protein YoaB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/15);  total (11/15)

GGGCAGCAATAAATCGAGCATTCTGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCA  >  NC_000913/1893525‑1893782                                                                                                                              |                                                                                                                                     gggCAGCAATAAATCGAGCATTCTGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTtga                                                                                                                        >  1:237486/1‑140 (MQ=255)  ggCAGCAATAAATCGAGCATTCTGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTtgat                                                                                                                       >  2:304923/1‑140 (MQ=255)      gcaATAAATCGAGCATTCTGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAAc                                                                                                                   <  2:237486/140‑1 (MQ=255)                        tGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGaa                                                                                                 >  2:383588/1‑140 (MQ=255)                                accaTTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGt                                                                                                     <  1:314969/128‑1 (MQ=255)                                accaTTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGt                                                                                                     >  2:314969/1‑128 (MQ=255)                                accaTTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGAt                                                                                         >  2:434188/1‑140 (MQ=255)                                       tCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCt                                                                                  <  1:383588/140‑1 (MQ=255)                                        ccTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTg                                                                                 <  1:463157/140‑1 (MQ=255)                                        ccTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTg                                                                                 <  2:697109/140‑1 (MQ=255)                                             ccGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGgtg                                                                            <  1:304923/140‑1 (MQ=255)                                              cGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGgtgt                                                                           >  1:782025/1‑140 (MQ=255)                                                 tAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAg                                                                        >  1:548945/1‑140 (MQ=255)                                                          cTTCGCGGCGATGAATAAAGCATGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGa                                                               >  2:208785/1‑140 (MQ=255)                                                            tCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAg                                                             <  2:782025/140‑1 (MQ=255)                                                                            aGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACg                                             >  1:471269/1‑140 (MQ=255)                                                                                 gggATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAAt                                        >  2:322201/1‑140 (MQ=255)                                                                                   gATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAAttt                                      >  2:108278/1‑140 (MQ=255)                                                                                   gATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAAttt                                      <  2:471269/140‑1 (MQ=255)                                                                                   gATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAAttt                                      <  2:673733/140‑1 (MQ=255)                                                                                   gATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAATATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAAttt                                      <  2:211141/140‑1 (MQ=255)                                                                                                  gcgGGTCATGCGCCGGTGCGCTGCACGGCACAATCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGt                       <  1:654860/140‑1 (MQ=255)                                                                                                      gTCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGt                   <  2:389462/140‑1 (MQ=255)                                                                                                       tCATGCGCCGGTGCGCTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGtt                  <  2:548945/140‑1 (MQ=255)                                                                                                                      cTGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGAcc   <  1:13521/140‑1 (MQ=255)                                                                                                                       tGCACGGCACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCa  <  2:106265/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                     GGGCAGCAATAAATCGAGCATTCTGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCA  >  NC_000913/1893525‑1893782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑