Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,988,066	C→T	intergenic (‑107/‑156)	azuC ← / → yecR	uncharacterized protein AzuC/lipoprotein YecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,988,066	0	C	T	100.0%	152.4 / NA	43	intergenic (‑107/‑156)	azuC/yecR	uncharacterized protein AzuC/lipoprotein YecR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/15);  total (28/15)

CATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCT  >  NC_000913/1987930‑1988197                                                                                                                                         |                                                                                                                                    cATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCtt                                                                                                                                  >  1:31987/1‑140 (MQ=255)   tACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTa                                                                                                                                >  1:379787/1‑140 (MQ=255)              aTTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATaaaa                                                                                                                     >  2:463930/1‑140 (MQ=255)                  tGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGa                                                                                                                 >  1:177916/1‑140 (MQ=255)                                 cgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAGCCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGc                                                                                                  <  1:84301/140‑1 (MQ=255)                                   gCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGccc                                                                                                <  2:31987/140‑1 (MQ=255)                                                         aGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGa                                                                          >  1:292785/1‑140 (MQ=255)                                                          ggTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGAt                                                                         >  1:126721/1‑140 (MQ=255)                                                           gtgtTATTGTCTTTGCGCTTGTAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATg                                                                        >  2:573617/1‑140 (MQ=255)                                                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATg                                                                        >  1:689273/1‑140 (MQ=255)                                                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATg                                                                        >  2:787783/1‑140 (MQ=255)                                                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATg                                                                        >  2:374578/1‑140 (MQ=255)                                                             gtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGc                                                                      <  2:184938/140‑1 (MQ=255)                                                              ttATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCa                                                                     >  1:306047/1‑140 (MQ=255)                                                                      tttGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAg                                                             >  1:552454/1‑140 (MQ=255)                                                                        tGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAgcg                                                           >  2:716131/1‑140 (MQ=255)                                                                        tGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTACAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAgcg                                                           >  1:169442/1‑140 (MQ=255)                                                                                     tGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTc                                              >  1:436461/1‑140 (MQ=255)                                                                                     tGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTc                                              >  1:174654/1‑140 (MQ=255)                                                                                               cacaAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGcc                                    >  2:317262/1‑140 (MQ=255)                                                                                               cacaAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGcc                                    >  1:187638/1‑140 (MQ=255)                                                                                               cacaAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGcc                                    >  2:30163/1‑140 (MQ=255)                                                                                               cacaAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGcc                                    >  2:464668/1‑140 (MQ=255)                                                                                                 caAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCAt                                  >  2:660148/1‑140 (MQ=255)                                                                                                 caAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCAt                                  <  1:660148/140‑1 (MQ=255)                                                                                                 caAAAATCAATCTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCAt                                  >  1:453633/1‑140 (MQ=255)                                                                                                      atcaatcTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCg                             <  2:552454/140‑1 (MQ=255)                                                                                                      atcaatcTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCg                             <  1:572630/140‑1 (MQ=255)                                                                                                      atcaatcTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCg                             <  1:317262/140‑1 (MQ=255)                                                                                                        caatcTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgca                           <  2:689273/140‑1 (MQ=255)                                                                                                        caatcTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgca                           <  2:292785/140‑1 (MQ=255)                                                                                                         aatcTTTATGTGATACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgcag                          <  2:306047/140‑1 (MQ=255)                                                                                                                     aTACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAcgcg              >  1:335862/1‑140 (MQ=255)                                                                                                                     aTACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAcgcg              >  1:396641/1‑140 (MQ=255)                                                                                                                     aTACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAc                 >  2:279963/1‑137 (MQ=255)                                                                                                                     aTACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAc                 <  1:279963/137‑1 (MQ=255)                                                                                                                      tACAAATCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGt             >  1:128352/1‑140 (MQ=255)                                                                                                                           aTCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAAt        >  1:139698/1‑140 (MQ=255)                                                                                                                            tCACATAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATa       <  2:379787/140‑1 (MQ=255)                                                                                                                               caTAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTg    <  1:464668/140‑1 (MQ=255)                                                                                                                                aTAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGc   >  1:754154/1‑140 (MQ=255)                                                                                                                                aTAAATACCTCTTTAATGTTACAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGc   <  2:169442/140‑1 (MQ=255)                                                                                                                                 tAAATACCTCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCt  <  2:436461/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCT  >  NC_000913/1987930‑1988197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑