Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,061,676	A→G	G86G (GGT→GGC)	nac ←	DNA‑binding transcriptional dual regulator Nac

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,061,676	0	A	G	100.0%	125.2 / NA	37	G86G (GGT→GGC)	nac	DNA‑binding transcriptional dual regulator Nac
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/18);  total (19/18)

CTATTTTCATGAAGATAGATAACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTC  >  NC_000913/2061548‑2061810                                                                                                                                 |                                                                                                                                       cTATTTTCATGAAGATAGATAACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATg                                                                                                                             >  1:358694/1‑140 (MQ=255) cTATTTTCATGAAGATAGATAACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATg                                                                                                                             >  2:420450/1‑140 (MQ=255)  tATTTTCATAAAGATAAATAACGATCCCCGGAAATTCAGCGGGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGc                                                                                                                            <  2:159410/140‑1 (MQ=255)    ttttCATGAAGATAGATAACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCAc                                                                                                                          >  1:143910/1‑140 (MQ=255)    ttttCATGAAGATAGATAACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCAc                                                                                                                          >  1:684819/1‑140 (MQ=255)      ttCATGAAGATAGATAACGATCTCCGAAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCg                                                                                                                        <  2:192971/140‑1 (MQ=255)         aTGAAGATAGATAACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCTCGATAATGCCTGGCCAACGTTATGCACCGCCa                                                                                                                     >  2:340935/1‑140 (MQ=255)               ttagataCCGATCCCCGAAAATGAAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCCCCGCCAGTTggg                                                                                                               <  2:143910/139‑1 (MQ=255)               atagataACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTggg                                                                                                               <  1:679993/140‑1 (MQ=255)               atagataACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTggg                                                                                                               <  2:788467/140‑1 (MQ=255)                aagataACGATCCCCGAAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGc                                                                                                              <  2:358694/139‑1 (MQ=255)                aagaaaccGATCTCCGAAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGc                                                                                                              <  2:684819/133‑1 (MQ=255)                         aTCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGc                                                                                                              <  2:55504/131‑1 (MQ=255)                         aTCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGc                                                                                                              >  1:55504/1‑131 (MQ=255)                         aTCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTcaca                                                                                                     >  2:793645/1‑140 (MQ=255)                           ctcCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACt                                                                                                   >  1:476166/1‑140 (MQ=255)                                   aTTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTaaa                                                                                           >  2:801446/1‑140 (MQ=255)                                     tCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAAt                                                                                         >  2:479893/1‑140 (MQ=255)                                         cgcgAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGcc                                                                                     >  2:435329/1‑140 (MQ=255)                                               ccGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCa                                                                               <  1:479893/140‑1 (MQ=255)                                                              gCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAAtt                                                                >  2:537523/1‑140 (MQ=255)                                                                aTGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAAtttt                                                              <  1:390423/140‑1 (MQ=255)                                                                aTGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAAtttt                                                              <  1:435329/140‑1 (MQ=255)                                                                aTGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAAtttt                                                              >  2:441336/1‑140 (MQ=255)                                                                 tGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAAttttt                                                             >  1:445642/1‑140 (MQ=255)                                                                 tGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAAttttt                                                             <  1:441336/140‑1 (MQ=255)                                                                       tggatgACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCg                                                       >  1:117217/1‑140 (MQ=255)                                                                              cGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTg                                                >  1:566761/1‑140 (MQ=255)                                                                                                  aGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTa                            >  2:367821/1‑140 (MQ=255)                                                                                                  aGCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTa                            <  2:566761/140‑1 (MQ=255)                                                                                                   gCCAATCGAGACTTGCCCCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTAc                           <  1:537523/140‑1 (MQ=255)                                                                                                                 gCCTCGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAgttgt             <  1:340935/140‑1 (MQ=255)                                                                                                                  ccccGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTa                            <  1:602622/124‑1 (MQ=255)                                                                                                                  ccccGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTa                            >  2:602622/1‑124 (MQ=255)                                                                                                                  ccccGATAATGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAattgtt            >  1:62536/1‑140 (MQ=255)                                                                                                                          aTGCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAAc    <  1:262930/140‑1 (MQ=255)                                                                                                                            gCCTGGCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTc  <  1:793645/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       CTATTTTCATGAAGATAGATAACGATCTCCGGAAATTCAGCGCGAACCGCCTGTAATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTC  >  NC_000913/2061548‑2061810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑