Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,178,044	T→C	E174G (GAG→GGG)	fbaB ←	fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,178,044	0	T	C	100.0%	165.1 / NA	48	E174G (GAG→GGG)	fbaB	fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/29);  total (19/29)

TCGGCGGAAACATGGTAATCAACGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGA  >  NC_000913/2177909‑2178179                                                                                                                                        |                                                                                                                                        tCGGCGGAAACATGGTAATCAACGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTc                                                                                                                                     >  2:588754/1‑140 (MQ=255) tCGGCGGAAACATGGTAATCAACGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTc                                                                                                                                     <  2:61696/140‑1 (MQ=255)     cggAAACATGGTAATCAACGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAg                                                                                                                                 <  1:60494/140‑1 (MQ=255)     cggAAACATGGTAATCAACGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAg                                                                                                                                 <  2:229642/140‑1 (MQ=255)     cggAAACATGGTAATCAACGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAg                                                                                                                                 <  1:293990/140‑1 (MQ=255)                       cGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCa                                                                                                               >  2:286368/1‑140 (MQ=255)                       cGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCa                                                                                                               <  1:286368/140‑1 (MQ=255)                             ctttcttAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACcaacc                                                                                                         <  2:434370/140‑1 (MQ=255)                                  ttAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACcaaccgcaac                                                                                                    <  2:33217/140‑1 (MQ=255)                                    aaaGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACcaaccgcaaccg                                                                                                  <  1:383398/140‑1 (MQ=255)                                       ggCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCgcgc                                                                                               <  2:513177/140‑1 (MQ=255)                                       ggCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCgcgc                                                                                               <  2:295144/140‑1 (MQ=255)                                                   cAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACg                                                                                   >  2:40148/1‑140 (MQ=255)                                                      aTAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCt                                                                                >  2:291944/1‑140 (MQ=255)                                                         ggCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCt                                                                             <  1:291944/140‑1 (MQ=255)                                                          gCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCt                                                                             <  2:684687/139‑1 (MQ=255)                                                          gCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCt                                                                             >  1:684687/1‑139 (MQ=255)                                                          gCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTc                                                                            >  2:410353/1‑140 (MQ=255)                                                          gCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTc                                                                            >  1:664344/1‑140 (MQ=255)                                                              acaGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACg                                                                        <  2:333181/140‑1 (MQ=255)                                                               caGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGc                                                                       >  1:263834/1‑140 (MQ=255)                                                               caGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGc                                                                       >  1:685387/1‑140 (MQ=255)                                                                      gTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATa                                                                <  2:664344/140‑1 (MQ=255)                                                                       tCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACg                                                                        <  1:271153/131‑1 (MQ=255)                                                                       tCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACg                                                                        >  2:271153/1‑131 (MQ=255)                                                                             tACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGtt                                                         <  2:232519/140‑1 (MQ=255)                                                                              aCCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGttt                                                        <  1:588754/140‑1 (MQ=255)                                                                               cccAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTg                                                       >  2:442590/1‑140 (MQ=255)                                                                                 cAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGt                                                          <  1:582723/135‑1 (MQ=255)                                                                                 cAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGt                                                          >  2:582723/1‑135 (MQ=255)                                                                                 cAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGAt                                                     >  1:94151/1‑140 (MQ=255)                                                                                   gCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATa                                                                >  1:146549/1‑127 (MQ=255)                                                                                   gCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATa                                                                <  2:146549/127‑1 (MQ=255)                                                                                    cTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGt                                                  <  2:94151/140‑1 (MQ=255)                                                                                    cTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGt                                                  >  1:357552/1‑140 (MQ=255)                                                                                     tCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTa                                                 >  2:575257/1‑140 (MQ=255)                                                                                        tGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGt                                              <  1:410353/140‑1 (MQ=255)                                                                                        tGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGt                                              <  2:685387/140‑1 (MQ=255)                                                                                         gcgcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGCTTGATCGTAGGTa                                             >  2:126072/1‑140 (MQ=255)                                                                                          cgcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTAt                                            <  1:516656/140‑1 (MQ=255)                                                                                          cgcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTAt                                            <  1:442590/140‑1 (MQ=255)                                                                                          cgcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGCTTGATCGTAGGTAt                                            >  2:451598/1‑140 (MQ=255)                                                                                               gTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCggg                                       <  2:465029/140‑1 (MQ=255)                                                                                               gTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCggg                                       <  1:365813/140‑1 (MQ=255)                                                                                                                                gACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACg      <  1:40148/140‑1 (MQ=255)                                                                                                                                gACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACg      <  2:263834/140‑1 (MQ=255)                                                                                                                                  cGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAg    <  1:575257/140‑1 (MQ=255)                                                                                                                                    tGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAgga  >  2:687474/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TCGGCGGAAACATGGTAATCAACGCCATCTTTCTTAAAGGCGGAGTTACGCAAATAGGCCCACAGCACTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGA  >  NC_000913/2177909‑2178179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑