Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,217,485	A→G	V358A (GTG→GCG)	yehY ←	glycine betaine ABC transporter membrane subunit YehY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,217,485	0	A	G	100.0%	86.0 / NA	26	V358A (GTG→GCG)	yehY	glycine betaine ABC transporter membrane subunit YehY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/9);  total (17/9)

GTTAACGGCTTTTTGTGCGCCGAACAGTTTGCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAG  >  NC_000913/2217354‑2217621                                                                                                                                    |                                                                                                                                         gTTAACGGCTTTTTGTGCGCCGAACAGTTTGCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGc                                                                                                                                  <  2:178269/140‑1 (MQ=255)    aaCGGCTTTTTGTGCGCCGAACAGTTTGCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAac                                                                                                                               >  1:406434/1‑140 (MQ=255)      cGGCTTTTTGTGCGCCGAACAGTTTGCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAacac                                                                                                                             >  2:521207/1‑140 (MQ=255)                   gcCGAACAGTTTGCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGgcg                                                                                                                <  1:316848/140‑1 (MQ=255)                   gcCGAACAGTTTGCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGgcg                                                                                                                <  2:721112/140‑1 (MQ=255)                      gAACAGTTTGCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCgctg                                                                                                             <  1:687785/140‑1 (MQ=255)                               gCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTagcagc                                                                                                    <  2:406434/140‑1 (MQ=255)                               gCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTagcagc                                                                                                    <  1:469866/140‑1 (MQ=255)                                cTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGcc                                                                                                   <  2:730936/140‑1 (MQ=255)                                 tGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGccc                                                                                                  >  2:646050/1‑140 (MQ=255)                                             aTTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAg                                                                                      <  1:646050/140‑1 (MQ=255)                                                   tCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCAcc                                                                                >  2:415255/1‑140 (MQ=255)                                                             tCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCt                                                                      >  1:686168/1‑140 (MQ=255)                                                             tCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCt                                                                      >  1:551103/1‑140 (MQ=255)                                                               aCCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTgc                                                                    >  1:699506/1‑140 (MQ=255)                                                                ccTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTgcg                                                                   <  1:521207/140‑1 (MQ=255)                                                                       cagTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTa                                                            >  2:704986/1‑140 (MQ=255)                                                                          tGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAAcg                                                         >  1:163225/1‑140 (MQ=255)                                                                           gCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAAcgc                                                        >  1:312059/1‑140 (MQ=255)                                                                                      aTCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCg                                             >  1:306105/1‑140 (MQ=255)                                                                                           aCAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATa                                        >  2:418860/1‑140 (MQ=255)                                                                                                 cgTCGGTAAGCACCGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACa                                  >  2:800670/1‑140 (MQ=255)                                                                                                              cGCCAGAACAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATca                     >  1:456034/1‑140 (MQ=255)                                                                                                                   gAACAATTACCGGGATCGCCCCCATCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCaccacc                >  2:267642/1‑140 (MQ=255)                                                                                                                    aaCAATTACCGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCAccc               >  2:13688/1‑140 (MQ=255)                                                                                                                            ccGGGATCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTg       >  1:757959/1‑140 (MQ=255)                                                                                                                                 aTCGCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAg  >  1:165431/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         GTTAACGGCTTTTTGTGCGCCGAACAGTTTGCTGACATGGCTAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAG  >  NC_000913/2217354‑2217621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑