Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,221,754	G→A	A79V (GCG→GTG)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,221,754	0	G	A	100.0%	156.7 / NA	47	A79V (GCG→GTG)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/20);  total (27/20)

GGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCATTTTCTTAAGCAGTTCGGTG  >  NC_000913/2221620‑2221876                                                                                                                                       |                                                                                                                           ggCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCa                                                                                                                       >  1:146555/1‑140 (MQ=255)       tCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGa                                                                                                                 <  2:42958/140‑1 (MQ=255)       tCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGa                                                                                                                 <  2:577289/140‑1 (MQ=255)        cGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGAc                                                                                                                <  1:246593/140‑1 (MQ=255)        cGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGAc                                                                                                                <  2:430812/140‑1 (MQ=255)            aaCACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTc                                                                                                            <  1:755430/140‑1 (MQ=255)                ccGTGCGCTGACCGTGCAGCATGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTg                                                                                                        >  2:355189/1‑140 (MQ=255)                   tGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAAc                                                                                                                     >  2:285463/1‑124 (MQ=255)                   tGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAAc                                                                                                                     <  1:285463/124‑1 (MQ=255)                   tGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGAc                                                                                                                <  1:186875/129‑1 (MQ=255)                   tGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGAc                                                                                                                >  2:186875/1‑129 (MQ=255)                   tGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGatc                                                                                                     >  2:166467/1‑140 (MQ=255)                         gACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTcg                                                                                               >  2:643999/1‑140 (MQ=255)                            cGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCgg                                                                                            <  1:643999/140‑1 (MQ=255)                              tGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGAt                                                                                          >  2:103964/1‑140 (MQ=255)                                cagcaCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCg                                                                                        >  1:528242/1‑140 (MQ=255)                                  gcaTGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGcc                                                                                      <  1:355189/140‑1 (MQ=255)                                   caCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCct                                                                                     >  2:50582/1‑140 (MQ=255)                                    aCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCctc                                                                                    >  1:679644/1‑140 (MQ=255)                                      gtcgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCtt                                                                                  >  1:778806/1‑140 (MQ=255)                                            aGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGt                                                                            >  2:620218/1‑140 (MQ=255)                                             gCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGtt                                                                           >  1:357005/1‑140 (MQ=255)                                                  gaaaagaGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCtt                                                                                  >  2:253147/1‑128 (MQ=255)                                                  gaaaagaGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCtt                                                                                  <  1:253147/128‑1 (MQ=255)                                                           aaTTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGc                                                             >  2:675817/1‑140 (MQ=255)                                                                  aGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTaa                                                      >  1:546705/1‑140 (MQ=255)                                                                   ggcggcTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAg                                                     <  2:357005/140‑1 (MQ=255)                                                                                  tCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAAtt                                      <  2:146555/140‑1 (MQ=255)                                                                                       tGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTtctc                                 >  2:566070/1‑140 (MQ=255)                                                                                        gATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCtca                                >  2:215603/1‑140 (MQ=255)                                                                                                  ggCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCa                      <  2:679644/140‑1 (MQ=255)                                                                                                  ggCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCa                      <  1:50582/140‑1 (MQ=255)                                                                                                  ggCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCa                      <  1:506358/140‑1 (MQ=255)                                                                                                   gcgcGGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCAt                     >  2:24508/1‑140 (MQ=255)                                                                                                    cgcgGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCAtt                    <  2:546705/140‑1 (MQ=255)                                                                                                    cgcgGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCAtt                    >  2:122856/1‑140 (MQ=255)                                                                                                    cgcgGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCAtt                    >  2:131638/1‑140 (MQ=255)                                                                                                    cgcgGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCAtt                    >  1:10907/1‑140 (MQ=255)                                                                                                    cgcgGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCAtt                    >  1:140168/1‑140 (MQ=255)                                                                                                     gcgGATATCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCAttt                   >  1:429176/1‑140 (MQ=255)                                                                                                         atatCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCATTTTCtt               >  2:157841/1‑140 (MQ=255)                                                                                                          tatCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCa                      <  2:217928/132‑1 (MQ=255)                                                                                                          tatCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCa                      >  1:217928/1‑132 (MQ=255)                                                                                                            tCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCATTTTCTTAAg            <  1:215603/140‑1 (MQ=255)                                                                                                            tCCTGACGGGTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCATTTTCTTAAg            <  1:122856/140‑1 (MQ=255)                                                                                                                     gTTACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCATTTTCTTAAGCAGTTCGGt   >  2:334810/1‑140 (MQ=255)                                                                                                                      ttACGGTGTTGAAAATCACCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCATTTTCTTAAGCAGTTCGGTg  <  1:157841/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                           GGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCATCTCGCGGATCGCCTCTTTCGGGTTATCCGGGCCGACGCTGATTAAGCGCAGCTGACCAATTTTCTCATCAACTGTCATTTTCTTAAGCAGTTCGGTG  >  NC_000913/2221620‑2221876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑