Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,229,799	G→A	P196L (CCG→CTG)	dusC ←	tRNA‑dihydrouridine(16) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,229,799	0	G	A	100.0%	136.1 / NA	39	P196L (CCG→CTG)	dusC	tRNA‑dihydrouridine(16) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/18);  total (21/18)

TTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCG  >  NC_000913/2229673‑2229927                                                                                                                               |                                                                                                                                 tttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCg                                                                                                                     <  2:734868/140‑1 (MQ=255)   tACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGca                                                                                                                   >  2:508379/1‑139 (MQ=255)   tACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCt                                                                                                                   >  1:679346/1‑140 (MQ=255)   tACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCt                                                                                                                   >  1:524609/1‑140 (MQ=255)        cccGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGa                                                                                                              >  2:517855/1‑140 (MQ=255)        cccGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGa                                                                                                              >  1:337429/1‑140 (MQ=255)                aGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGcc                                                                                                      >  1:287696/1‑140 (MQ=255)                aGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGcc                                                                                                      <  1:517855/140‑1 (MQ=255)                     gggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCg                                                                                                 >  1:163923/1‑140 (MQ=255)                         atatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCAGGCGAATATCGCCAATCGCCTg                                                                                             <  1:508379/140‑1 (MQ=255)                           atTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGcc                                                                                           <  1:17331/140‑1 (MQ=255)                            ttGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCa                                                                                          >  1:194048/1‑140 (MQ=255)                            ttGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCa                                                                                          >  1:716076/1‑140 (MQ=255)                            ttGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCa                                                                                          >  2:500375/1‑140 (MQ=255)                               aGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTc                                                                                       >  1:667346/1‑140 (MQ=255)                                gcgcCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCa                                                                                      >  2:424807/1‑140 (MQ=255)                                    cccGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAg                                                                                         >  1:276650/1‑133 (MQ=255)                                    cccGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAg                                                                                         <  2:276650/133‑1 (MQ=255)                                    cccGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAatat                                                                                  >  1:728747/1‑140 (MQ=255)                                       gcgACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCt                                                                               <  2:524609/140‑1 (MQ=255)                                               tcatcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCgg                                                                       <  2:728747/140‑1 (MQ=255)                                                   caCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAc                                                                   >  1:799423/1‑140 (MQ=255)                                                    aCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAcc                                                                  >  2:739519/1‑140 (MQ=255)                                                      tGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCt                                                                <  2:608622/140‑1 (MQ=255)                                                        cGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGc                                                              >  1:744192/1‑140 (MQ=255)                                                                 cGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTc                                                     <  2:744192/140‑1 (MQ=255)                                                                   cTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCg                                                   <  1:500375/140‑1 (MQ=255)                                                                    tGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGc                                                  <  2:337429/140‑1 (MQ=255)                                                                       tCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCa                                               <  2:799423/140‑1 (MQ=255)                                                                          ccATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGc                                            <  1:424603/140‑1 (MQ=255)                                                                          ccATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGc                                            >  2:77130/1‑140 (MQ=255)                                                                               cATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcaccac                                       <  1:35386/140‑1 (MQ=255)                                                                                       cgcgcTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCg                               <  1:77130/140‑1 (MQ=255)                                                                                         cgcTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCa                                               <  2:312490/122‑1 (MQ=255)                                                                                         cgcTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCa                                               >  1:312490/1‑122 (MQ=255)                                                                                               gCCAGTCCCAGATTTCACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCa                       <  1:739519/140‑1 (MQ=255)                                                                                                              cACCGTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCa        >  2:737645/1‑140 (MQ=255)                                                                                                                  gTTGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCgg    <  1:131822/140‑1 (MQ=255)                                                                                                                    tGGCAATCACCAGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCg  >  1:747308/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                 TTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCG  >  NC_000913/2229673‑2229927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑