Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,336,956	A→G	V822A (GTG→GCG)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,336,956	0	A	G	98.2%	195.6 / ‑5.9	57	V822A (GTG→GCG)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (24/32);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGT  >  NC_000913/2336821‑2337092                                                                                                                                        |                                                                                                                                         tCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGcc                                                                                                                                      <  1:787940/140‑1 (MQ=255)           gAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTa                                                                                                                            >  1:475385/1‑140 (MQ=255)               gATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGc                                                                                                                        >  2:704743/1‑140 (MQ=255)                aTTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGcc                                                                                                                       >  2:514517/1‑140 (MQ=255)                        gTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGc                                                                                                               >  2:184294/1‑140 (MQ=255)                           cccTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGa                                                                                                            >  1:300349/1‑140 (MQ=255)                                   cgGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGaa                                                                                                    >  2:431043/1‑140 (MQ=255)                                   cgGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGaa                                                                                                    >  2:259480/1‑140 (MQ=255)                                      cACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAAcg                                                                                                 >  1:437949/1‑140 (MQ=255)                                         tGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGa                                                                                              <  1:514517/140‑1 (MQ=255)                                                gATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAgt                                                                                            >  1:27378/1‑135 (MQ=255)                                                gATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAgt                                                                                            <  2:27378/135‑1 (MQ=255)                                                 aTGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAgtacgtac                                                                                      <  2:517136/140‑1 (MQ=255)                                                        ccAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTa                                                                               >  1:200112/1‑140 (MQ=255)                                                         cAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTAc                                                                              <  2:475385/140‑1 (MQ=255)                                                            aTCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCgg                                                                           <  1:533634/140‑1 (MQ=255)                                                               ttCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTAc                                                                              >  2:163458/1‑134 (MQ=255)                                                               ttCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTAc                                                                              <  1:163458/134‑1 (MQ=255)                                                               ttCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCAt                                                                        >  2:335738/1‑140 (MQ=255)                                                                 ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTAcc                                                                             <  1:435236/133‑1 (MQ=255)                                                                 ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTAcc                                                                             >  2:435236/1‑133 (MQ=255)                                                                 ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCg                                                                      >  1:413337/1‑140 (MQ=255)                                                                 ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCg                                                                      >  2:267084/1‑140 (MQ=255)                                                                 ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCg                                                                      >  2:595225/1‑140 (MQ=255)                                                                   tCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCATTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGt                                                                    <  1:632020/140‑1 (MQ=255)                                                                   tCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGt                                                                    <  1:322764/140‑1 (MQ=255)                                                                     gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGa                                                                  <  2:437949/140‑1 (MQ=255)                                                                     gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGa                                                                  <  1:595225/140‑1 (MQ=255)                                                                     gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGa                                                                  <  2:763040/140‑1 (MQ=255)                                                                     gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGa                                                                  <  1:312727/140‑1 (MQ=255)                                                                      tCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGat                                                                 <  2:200112/140‑1 (MQ=255)                                                                                cAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATc                                                       <  1:184294/140‑1 (MQ=255)                                                                                        cGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCa                                               >  1:631796/1‑140 (MQ=255)                                                                                        cGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCa                                               >  1:537853/1‑140 (MQ=255)                                                                                        cGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCa                                               <  2:300349/140‑1 (MQ=255)                                                                                         gTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAg                                              <  2:413337/140‑1 (MQ=255)                                                                                           tGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTc                                            <  1:267084/140‑1 (MQ=255)                                                                                            gCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCa                                           <  1:678053/140‑1 (MQ=255)                                                                                            gCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCa                                           <  1:335738/140‑1 (MQ=255)                                                                                            gCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCa                                           <  2:718137/140‑1 (MQ=255)                                                                                            gCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCa                                           <  2:354201/140‑1 (MQ=255)                                                                                                  ccACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTAcc                                     <  1:431043/140‑1 (MQ=255)                                                                                                   cACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCt                                    >  1:116604/1‑140 (MQ=255)                                                                                                     cTACGTTTTCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGt                                  >  2:171966/1‑140 (MQ=255)                                                                                                            ttCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCgcgc                           >  1:777157/1‑140 (MQ=255)                                                                                                            ttCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCgcgc                           >  1:189351/1‑140 (MQ=255)                                                                                                             tCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGcc                          <  2:189351/140‑1 (MQ=255)                                                                                                             tCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGcc                          <  1:303637/140‑1 (MQ=255)                                                                                                             tCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGcc                          <  2:631796/140‑1 (MQ=255)                                                                                                             tCATCTTCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGcc                          <  2:788092/140‑1 (MQ=255)                                                                                                                   tCCGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCaacaac                    >  2:77947/1‑140 (MQ=255)                                                                                                                     cGCAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTa                  <  1:171966/140‑1 (MQ=255)                                                                                                                       cAGTACGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaaa                >  2:570969/1‑140 (MQ=255)                                                                                                                           aCGGATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGt            <  1:618784/140‑1 (MQ=255)                                                                                                                             ggATGAGGATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTa          <  1:259480/140‑1 (MQ=255)                                                                                                                                   ggATCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCg    <  2:777157/140‑1 (MQ=255)                                                                                                                                     aTCGCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTGCCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGt  <  1:197192/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGT  >  NC_000913/2336821‑2337092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑