Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,388,168	T→C	I87V (ATT→GTT)	yfbN ←	uncharacterized protein YfbN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,388,168	0	T	C	100.0%	274.4 / NA	77	I87V (ATT→GTT)	yfbN	uncharacterized protein YfbN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/42);  total (35/42)

ATGTTTGTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAATCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGT  >  NC_000913/2388033‑2388302                                                                                                                                        |                                                                                                                                       aTGTTTGTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAAt                                                                                                                                    <  2:265487/140‑1 (MQ=255)      tGTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGt                                                                                                                               >  2:559013/1‑140 (MQ=255)      tGTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGt                                                                                                                               <  1:588198/140‑1 (MQ=255)      tGTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGt                                                                                                                               >  1:133615/1‑140 (MQ=255)       gTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTc                                                                                                                              <  2:355411/140‑1 (MQ=255)       gTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTc                                                                                                                              <  1:732609/140‑1 (MQ=255)       gTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTc                                                                                                                              <  2:596238/140‑1 (MQ=255)       gTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTc                                                                                                                              <  2:508814/140‑1 (MQ=255)        tAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCa                                                                                                                             <  2:702454/140‑1 (MQ=255)        tAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCa                                                                                                                             <  2:451778/140‑1 (MQ=255)              tGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGtat                                                                                                                       >  1:328562/1‑140 (MQ=255)                gCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAg                                                                                                                     >  2:685302/1‑140 (MQ=255)                gCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAg                                                                                                                     >  2:104462/1‑140 (MQ=255)                              atatCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAAt                                                                                                       >  2:768878/1‑140 (MQ=255)                              atatCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAAt                                                                                                       >  2:434970/1‑140 (MQ=255)                              atatCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAAt                                                                                                       <  1:607206/140‑1 (MQ=255)                                 tCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACg                                                                                                    >  1:229583/1‑140 (MQ=255)                                  cTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGc                                                                                                   >  1:587558/1‑140 (MQ=255)                                     cAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGa                                                                                                <  2:574783/140‑1 (MQ=255)                                     cAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGa                                                                                                <  1:183365/140‑1 (MQ=255)                                      aaaaCCAAACCAAAACTTACGCTTGAGATCCCTCTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATTCGCCGaa                                                                                               <  1:615058/140‑1 (MQ=255)                                       aaaccaaaccAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAag                                                                                              <  2:624765/140‑1 (MQ=255)                                        aaccaaaccaAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAaga                                                                                             <  1:559013/140‑1 (MQ=255)                                        aaccaaaccaAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAaga                                                                                             >  1:448628/1‑140 (MQ=255)                                           caaaccaaaACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCt                                                                                          <  2:328562/140‑1 (MQ=255)                                             aaccaaaACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTc                                                                                        <  2:269423/140‑1 (MQ=255)                                             aaccaaaACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTc                                                                                        <  1:406226/140‑1 (MQ=255)                                               ccaaaACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTg                                                                                      <  2:517126/140‑1 (MQ=255)                                                   aaCTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAat                                                                                  >  1:781860/1‑140 (MQ=255)                                                       tACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCa                                                                              <  2:448628/140‑1 (MQ=255)                                                        aCGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCa                                                                                    <  2:592543/133‑1 (MQ=255)                                                        aCGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCa                                                                                    >  1:592543/1‑133 (MQ=255)                                                                   cccACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGcc                                                                  >  2:199548/1‑140 (MQ=255)                                                                         cATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACaaa                                                            <  2:781860/140‑1 (MQ=255)                                                                          atatTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACaaaa                                                           >  2:89671/1‑140 (MQ=255)                                                                             ttCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTa                                                                     >  2:634782/1‑127 (MQ=255)                                                                             ttCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTa                                                                     <  1:634782/127‑1 (MQ=255)                                                                                aaTCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATtct                                                     >  2:517138/1‑140 (MQ=255)                                                                                  tCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTctct                                                   >  1:208712/1‑140 (MQ=255)                                                                                       aaTGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTc                                              <  1:298321/140‑1 (MQ=255)                                                                                              tatTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGc                                                 <  2:484355/130‑1 (MQ=255)                                                                                              tatTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGc                                                 >  1:484355/1‑130 (MQ=255)                                                                                              tatTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCtgatga                                       <  1:89671/140‑1 (MQ=255)                                                                                              tatTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCtgatga                                       >  2:247784/1‑140 (MQ=255)                                                                                                   tttAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTctct                                                   >  2:549917/1‑123 (MQ=255)                                                                                                   tttAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTctct                                                   <  1:549917/123‑1 (MQ=255)                                                                                                   tttAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGAc                                  >  1:132108/1‑140 (MQ=255)                                                                                                   tttAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGAc                                  >  1:164063/1‑140 (MQ=255)                                                                                                   tttAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGAc                                  >  2:135672/1‑140 (MQ=255)                                                                                                   tttAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGAc                                  >  1:44620/1‑140 (MQ=255)                                                                                                   tttAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGAc                                  >  1:662047/1‑140 (MQ=255)                                                                                                     tAAGGCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGt                                >  2:120717/1‑140 (MQ=255)                                                                                                        ggCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTg                             <  2:208712/140‑1 (MQ=255)                                                                                                        ggCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTg                             >  1:164376/1‑140 (MQ=255)                                                                                                        ggCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTg                             >  1:296572/1‑140 (MQ=255)                                                                                                        ggCATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTg                             >  1:364075/1‑140 (MQ=255)                                                                                                          cATCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCt                           >  2:666045/1‑140 (MQ=255)                                                                                                           aTCTCCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCtt                          <  1:120717/140‑1 (MQ=255)                                                                                                             ctcCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATTAGTGACGTCTGCtttt                        >  1:605796/1‑140 (MQ=255)                                                                                                             ctcCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCtttt                        <  1:199548/140‑1 (MQ=255)                                                                                                             ctcCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCtt                          >  1:785943/1‑138 (MQ=255)                                                                                                             ctcCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCtt                          <  2:785943/138‑1 (MQ=255)                                                                                                              tcCGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTg                       <  2:410771/140‑1 (MQ=255)                                                                                                               ccGGCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGc                      <  1:666045/140‑1 (MQ=255)                                                                                                                 ggCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCg                    <  2:229583/140‑1 (MQ=255)                                                                                                                  gCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGt                   <  1:685302/140‑1 (MQ=255)                                                                                                                  gCATCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGt                   <  1:579309/140‑1 (MQ=255)                                                                                                                    aTCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCa                 <  2:614925/140‑1 (MQ=255)                                                                                                                    aTCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCa                 <  2:38694/140‑1 (MQ=255)                                                                                                                     tCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGc                      <  1:167236/134‑1 (MQ=255)                                                                                                                     tCCTTATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGc                      >  2:167236/1‑134 (MQ=255)                                                                                                                        ttATTTTTACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAatat             >  2:702642/1‑140 (MQ=255)                                                                                                                              ttACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGAt       <  2:662047/140‑1 (MQ=255)                                                                                                                              ttACGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGAt       <  2:296572/140‑1 (MQ=255)                                                                                                                                aCGCAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGAttt     >  1:171531/1‑140 (MQ=255)                                                                                                                                   cAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGt  <  2:171531/140‑1 (MQ=255)                                                                                                                                   cAAAACCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGt  <  1:135672/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ATGTTTGTAAAACTGGCGGTAATAATTGGATATCTGCAAAACCAAACCAAAACTTACGCTTGAGATCCCACTCATATTCAATCGCCAATGCTATATTGTTTAAGGCATCTCCGGCATCCTTATTTTTACGCAAAATCAATTCCGTCAGTGTATAGCGTTGTTCATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGT  >  NC_000913/2388033‑2388302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑