Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	226,266	A→G	noncoding (508/2904 nt)	rrlH →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	226,266	0	A	G	100.0%	113.0 / NA	50	noncoding (508/2904 nt)	rrlH	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/26);  total (24/26)

GGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGA  >  NC_000913/226165‑226395                                                                                                      |                                                                                                                                  gggggACCATCCTCCAAGGCTAAATACTCCTGACTGACCGTTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCtt                                                                                             >  1:295870/1‑140 (MQ=17) gggggACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCtt                                                                                             <  2:197361/140‑1 (MQ=18) gggggACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCtt                                                                                             >  2:670668/1‑140 (MQ=17) gggggACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCtt                                                                                             >  1:89144/1‑140 (MQ=17) gggggACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCtt                                                                                             >  1:612623/1‑140 (MQ=17)     gACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGc                                                                                         >  1:426191/1‑140 (MQ=17)          tcctccAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGtgtg                                                                                    >  1:165400/1‑140 (MQ=17)          tcctccAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGtgtg                                                                                    <  2:116250/140‑1 (MQ=18)                   gCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTAc                                                                           <  2:612623/140‑1 (MQ=18)                    cTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTAcc                                                                          <  1:14872/140‑1 (MQ=18)                    cTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTAcc                                                                          >  1:455544/1‑140 (MQ=17)                     tAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTAcc                                                                          >  1:602403/1‑139 (MQ=17)                     tAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTAcc                                                                          <  2:602403/139‑1 (MQ=18)                      aaaTACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCtt                                                                        <  2:455544/140‑1 (MQ=18)                       aaTACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCttt                                                                       >  2:106132/1‑140 (MQ=17)                              ctgactgaCCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATaa                                                                <  1:8991/140‑1 (MQ=18)                                     acCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCa                                                         <  2:426191/140‑1 (MQ=18)                                     acCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCa                                                         <  1:106132/140‑1 (MQ=18)                                      ccGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAg                                                        >  2:473046/1‑140 (MQ=17)                                       cGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc                                                       <  1:388012/140‑1 (MQ=18)                                       cGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc                                                       <  1:696669/140‑1 (MQ=18)                                       cGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGc                                                       <  2:165400/140‑1 (MQ=18)                                             tGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTta                                                 >  2:627935/1‑140 (MQ=17)                                                  cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTc                                            <  1:3436/140‑1 (MQ=17)                                                    gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAGTGGGTCa                                                         >  1:569494/1‑125 (MQ=17)                                                    gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCa                                                         <  2:569494/125‑1 (MQ=18)                                                    gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtata                                               <  1:210765/135‑1 (MQ=17)                                                    gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtata                                               >  2:210765/1‑135 (MQ=17)                                                    gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTg                                          >  1:351108/1‑140 (MQ=17)                                                    gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTg                                          >  2:403166/1‑140 (MQ=17)                                                          tGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCaa                                    >  1:518359/1‑140 (MQ=17)                                                           gAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAg                                   <  1:670668/140‑1 (MQ=17)                                                           gAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAg                                   <  2:492012/140‑1 (MQ=17)                                                            aGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAgg                                  >  2:363775/1‑140 (MQ=17)                                                             gggAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGt                                 <  1:733684/140‑1 (MQ=17)                                                             gggAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGt                                 <  2:661255/140‑1 (MQ=17)                                                             gggAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGt                                 >  1:661255/1‑140 (MQ=17)                                                               gAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTa                               >  1:439693/1‑140 (MQ=17)                                                                aaaGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTaa                              <  1:783465/140‑1 (MQ=17)                                                                aaaGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTaa                              <  2:439693/140‑1 (MQ=17)                                                                aaaGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTaa                              <  1:520902/140‑1 (MQ=17)                                                                        aaaGAACCCCGGCGAGGGGAGTGACAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAg                      >  1:404828/1‑140 (MQ=17)                                                                        aaaGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAg                      >  2:28887/1‑140 (MQ=17)                                                                              ccccGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGTGGAGc                >  1:547843/1‑140 (MQ=17)                                                                              ccccGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGc                <  2:518359/140‑1 (MQ=17)                                                                              ccccGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGc                <  1:28887/140‑1 (MQ=17)                                                                              ccccGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGc                >  2:701823/1‑140 (MQ=17)                                                                                         ggAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAAc     <  1:701823/140‑1 (MQ=17)                                                                                         ggAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAAc     <  1:403166/140‑1 (MQ=17)                                                                                            gTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGa  >  1:682614/1‑140 (MQ=17)                                                                                                      |                                                                                                                                  GGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGA  >  NC_000913/226165‑226395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑