Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,435	G→A	T37T (ACG→ACA)	folX →	dihydroneopterin triphosphate 2'‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,435	0	G	A	100.0%	48.6 / NA	15	T37T (ACG→ACA)	folX	dihydroneopterin triphosphate 2'‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/1);  total (14/1)

CTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGG  >  NC_000913/2421299‑2421568                                                                                                                                         |                                                                                                                                      ttAAGAAAAACAACGGAGAACCTGCAATGGCACACCCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATc                                                                                                                                    <  2:386905/139‑1 (MQ=255)    aGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATCCAc                                                                                                                                 >  2:233626/1‑140 (MQ=255)    aGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATCCAc                                                                                                                                 >  1:765103/1‑140 (MQ=255)    aGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATCCAc                                                                                                                                 >  1:125328/1‑140 (MQ=255)                     ccTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATCCACTACCCCGCCGATAAAgc                                                                                                                >  1:235589/1‑140 (MQ=255)                          aaggccacaCCCTGCCTTTTTTATTCATAAAAAGACCCTTCTTTTGCGTACTTTTATCGAAATAAAGAAAAAAAAAATAAACACCCGTCAGATTTTTTTTATAAATGTAACAACCCCCTCCCCCGCCGATAAAGCGCGCa                                                                                                           <  2:666017/135‑1 (MQ=255)                               cacaACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATCCACTACCCCGCCGATAAAGCGCGCACTAGc                                                                                                      >  1:666017/1‑140 (MQ=255)                               cacaACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATCCACTACCCCGCCGATAAAGCGCGCACTAGc                                                                                                      >  1:304203/1‑140 (MQ=255)                                acaACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCg                                                                                                     >  1:478043/1‑140 (MQ=255)                                                  tctaaaaaaGACCTTTCTTTTGCTTTCTTTTATGGAAATAAAGAAAAAAAAAAAAAACACCCGTCAGGTTTTTGTTATAAATGTAACAACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATgcgc                                                                                   <  2:235589/134‑1 (MQ=255)                                                                 tCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCg                                                                    >  1:525040/1‑140 (MQ=255)                                                                                         aaaGGAAGAAAAAATTAACAACCGTCAGGATATTGTTATCAATGTGACAACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATg                                            <  1:141896/139‑1 (MQ=255)                                                                                                                  cAGGATATTGTTATCAATGTGACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCt                   >  1:364849/1‑140 (MQ=255)                                                                                                                        atTGTTATCAATGTGACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaaaa             >  1:196380/1‑140 (MQ=255)                                                                                                                               tCAATGTGACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACt      >  2:478503/1‑140 (MQ=255)                                                                                                                               aaaaTGTAACAACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACt      <  2:364849/138‑1 (MQ=255)                                                                                                                                   tgtgACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACt      <  2:219785/136‑1 (MQ=255)                                                                                                                                   tgtgACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACt      >  1:219785/1‑136 (MQ=255)                                                                                                                                   tgtgACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAgg  >  1:331139/1‑140 (MQ=255)                                                                                                                                   tgtgACAATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAgg  >  2:182737/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGG  >  NC_000913/2421299‑2421568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑