Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,500,067	T→C	V466A (GTC→GCC)	ypdA →	sensory histidine kinase YpdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,500,067	0	T	C	100.0%	226.8 / NA	64	V466A (GTC→GCC)	ypdA	sensory histidine kinase YpdA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/31);  total (33/31)

AATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGC  >  NC_000913/2499936‑2500199                                                                                                                                    |                                                                                                                                     aaTTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTa                                                                                                                              >  1:232948/1‑140 (MQ=255)   ttAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTAtt                                                                                                                            <  1:108229/140‑1 (MQ=255)   ttAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTAtt                                                                                                                            <  2:572559/140‑1 (MQ=255)      aaGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAg                                                                                                                         <  1:467742/140‑1 (MQ=255)           tataTTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTg                                                                                                                    >  2:575726/1‑140 (MQ=255)            atatTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGc                                                                                                                   >  2:477948/1‑140 (MQ=255)            atatTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGc                                                                                                                   <  2:62886/140‑1 (MQ=255)                 gCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAgg                                                                                                              <  1:575726/140‑1 (MQ=255)                   cATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTa                                                                                                            >  2:343871/1‑140 (MQ=255)                   cATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTa                                                                                                            >  1:202167/1‑140 (MQ=255)                       gAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGc                                                                                                                        <  2:354967/124‑1 (MQ=255)                       gAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGc                                                                                                                        >  1:354967/1‑124 (MQ=255)                       gAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAgg                                                                                                        >  2:543338/1‑140 (MQ=255)                                 gCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCAcc                                                                                              <  2:543073/140‑1 (MQ=255)                                  cTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCa                                                                                             >  1:451376/1‑140 (MQ=255)                                  cTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCa                                                                                             >  1:60841/1‑140 (MQ=255)                                  cTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCa                                                                                             >  2:764939/1‑140 (MQ=255)                                  cTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCa                                                                                             >  2:753630/1‑140 (MQ=255)                                   tttGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCAt                                                                                            <  1:343871/140‑1 (MQ=255)                                     tGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCa                                                                                          <  1:543338/140‑1 (MQ=255)                                       gTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGc                                                                                        >  1:95340/1‑140 (MQ=255)                                             aGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa                                                                                  >  2:157335/1‑140 (MQ=255)                                             aGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa                                                                                  <  1:764939/140‑1 (MQ=255)                                             aGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa                                                                                  >  1:678218/1‑140 (MQ=255)                                             aGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa                                                                                  >  1:263940/1‑140 (MQ=255)                                                      ttATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGa                                                                         >  1:405220/1‑140 (MQ=255)                                                         tCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa                                                                                  <  2:683521/128‑1 (MQ=255)                                                         tCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCa                                                                                  >  1:683521/1‑128 (MQ=255)                                                            aTGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCgg                                                                   >  2:616680/1‑140 (MQ=255)                                                              gATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGt                                                                 <  1:611337/140‑1 (MQ=255)                                                                tatTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTAc                                                               >  2:121484/1‑140 (MQ=255)                                                                   tgatgaAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCa                                                            >  1:25833/1‑140 (MQ=255)                                                                     atgaAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt                                                          >  1:179289/1‑140 (MQ=255)                                                                        aaGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg                                                       <  1:121484/140‑1 (MQ=255)                                                                        aaGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg                                                       <  2:95340/140‑1 (MQ=255)                                                                        aaGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg                                                       <  2:25833/140‑1 (MQ=255)                                                                        aaGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg                                                       <  1:505990/140‑1 (MQ=255)                                                                          gagGTGAACTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGt                                                     >  2:429221/1‑140 (MQ=255)                                                                                     ctgcATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCg                                          <  1:268196/140‑1 (MQ=255)                                                                                       gcATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCggg                                        >  1:636759/1‑140 (MQ=255)                                                                                          ttCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCAc                                     >  2:164422/1‑140 (MQ=255)                                                                                            cccAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACgg                                   >  2:498893/1‑140 (MQ=255)                                                                                            cccAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACgg                                   >  2:582635/1‑140 (MQ=255)                                                                                               aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                <  2:232948/140‑1 (MQ=255)                                                                                               aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                <  2:678218/140‑1 (MQ=255)                                                                                               aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                <  2:202167/140‑1 (MQ=255)                                                                                               aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                <  2:451376/140‑1 (MQ=255)                                                                                               aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                <  2:456624/140‑1 (MQ=255)                                                                                               aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                <  2:179289/140‑1 (MQ=255)                                                                                               aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                <  1:477948/140‑1 (MQ=255)                                                                                               aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                <  1:35568/140‑1 (MQ=255)                                                                                                   tgctgATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGAt                            <  1:164422/140‑1 (MQ=255)                                                                                                    gctgATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATc                           >  1:157435/1‑140 (MQ=255)                                                                                                     ctgATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGAt                            <  1:219435/138‑1 (MQ=255)                                                                                                     ctgATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGAt                            >  2:219435/1‑138 (MQ=255)                                                                                                     ctgATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATcc                          >  2:166995/1‑140 (MQ=255)                                                                                                     ctgATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATcc                          >  2:10703/1‑140 (MQ=255)                                                                                                       gATCCAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCaa                        <  2:405220/140‑1 (MQ=255)                                                                                                          ccAGCCGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAgg                     >  1:391320/1‑140 (MQ=255)                                                                                                              ccGTTGGTGGAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCGGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGAt                 >  2:136785/1‑140 (MQ=255)                                                                                                                  tggtggAGAACGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAg             <  2:391320/140‑1 (MQ=255)                                                                                                                            cGCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAg   >  1:754949/1‑140 (MQ=255)                                                                                                                             gCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCGGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGc  <  1:136785/140‑1 (MQ=255)                                                                                                                             gCCATTGCCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGc  <  1:166995/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     AATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGC  >  NC_000913/2499936‑2500199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑