Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,524,341	C→T	G180S (GGT→AGT)	xapA ←	xanthosine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,524,341	0	C	T	97.4%	258.6 / ‑2.3	77	G180S (GGT→AGT)	xapA	xanthosine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (33/42);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CTGAAATAACCTCAGGCACCACAGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATG  >  NC_000913/2524209‑2524473                                                                                                                                     |                                                                                                                                     cTGAAATAACCTCAGGCACCACAGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCtcttct                                                                                                                               >  2:577755/1‑140 (MQ=255)  tGAAATAACCTCAGGCACCACAGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTcttctt                                                                                                                              >  2:241950/1‑140 (MQ=255)        aaCCTCAGGCACCACAGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCa                                                                                                                        <  1:241950/140‑1 (MQ=255)          ccTCAGGCACCACAGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACt                                                                                                                      <  1:473047/140‑1 (MQ=255)          ccTCAGGCACCACAGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACt                                                                                                                      <  1:41712/140‑1 (MQ=255)           cTCAGGCACCACAGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACtt                                                                                                                     >  1:118293/1‑140 (MQ=255)                    cacaGACATACCAACAACATCACCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACa                                                                                                            >  2:570303/1‑140 (MQ=255)                       aGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTg                                                                                                         <  2:542008/140‑1 (MQ=255)                         aCATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTgcg                                                                                                       >  1:391250/1‑140 (MQ=255)                         aCATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTgcg                                                                                                       <  1:775427/140‑1 (MQ=255)                         aCATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTgcg                                                                                                       <  1:681262/140‑1 (MQ=255)                           aTACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGcgcg                                                                                                     >  1:294253/1‑140 (MQ=255)                              ccAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTaa                                                                                                              >  1:198923/1‑128 (MQ=255)                              ccAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTaa                                                                                                              <  2:198923/128‑1 (MQ=255)                                   aacaTCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCg                                                                                             <  2:118293/140‑1 (MQ=255)                                     caTCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCa                                                                                           >  1:571161/1‑140 (MQ=255)                                     caTCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCa                                                                                           >  2:118674/1‑140 (MQ=255)                                       tCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATc                                                                                         >  1:61766/1‑140 (MQ=255)                                        ccccACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGt                                                                                                          <  1:395122/122‑1 (MQ=255)                                        ccccACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGt                                                                                                          >  2:395122/1‑122 (MQ=255)                                        ccccACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCg                                                                                             <  2:425448/135‑1 (MQ=255)                                        ccccACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCg                                                                                             >  1:425448/1‑135 (MQ=255)                                        ccccACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCg                                                                                        >  2:181816/1‑140 (MQ=255)                                        ccccACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCg                                                                                        >  1:322758/1‑140 (MQ=255)                                        ccccACCAATAA‑TTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGt                                                                                       >  1:433068/1‑140 (MQ=255)                                         cccACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGt                                                                                       >  2:795600/1‑140 (MQ=255)                                           caccaATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAg                                                                                     <  1:38227/140‑1 (MQ=255)                                             ccaATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGc                                                                                   >  1:474861/1‑140 (MQ=255)                                                ataatTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCAtt                                                                                >  1:139719/1‑140 (MQ=255)                                                 taatTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCAttt                                                                               <  2:139719/140‑1 (MQ=255)                                                   aattGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGc                                                                             <  2:433068/138‑1 (MQ=255)                                                     ttGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCa                                                                           <  1:30522/140‑1 (MQ=255)                                                     ttGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCa                                                                           <  2:391250/140‑1 (MQ=255)                                                      tGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAg                                                                          <  2:294253/140‑1 (MQ=255)                                                        catcatGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTg                                                                              >  1:567444/1‑134 (MQ=255)                                                        catcatGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTg                                                                              <  2:567444/134‑1 (MQ=255)                                                        catcatGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTg                                                                        >  1:620715/1‑140 (MQ=255)                                                        catcatGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTg                                                                        >  2:751072/1‑140 (MQ=255)                                                        catcatGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTg                                                                        <  1:181816/140‑1 (MQ=255)                                                         atcatGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTga                                                                       >  2:70028/1‑140 (MQ=255)                                                            atGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGc                                                                             >  1:271928/1‑131 (MQ=255)                                                            atGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGc                                                                             <  2:271928/131‑1 (MQ=255)                                                             tGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGagaag                                                                   >  2:440791/1‑140 (MQ=255)                                                             tGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGagaag                                                                   >  2:587552/1‑140 (MQ=255)                                                             tGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGagaag                                                                   >  2:313754/1‑140 (MQ=255)                                                             tGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGagaag                                                                   >  2:474945/1‑140 (MQ=255)                                                                  aTTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAgcg                                                              <  2:426111/140‑1 (MQ=255)                                                                  aTTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAgcg                                                              <  1:9636/140‑1 (MQ=255)                                                                   tttCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAgcgc                                                             <  1:227937/140‑1 (MQ=255)                                                                    ttCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGct                                                            <  1:118674/140‑1 (MQ=255)                                                                    ttCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGct                                                            <  2:620715/140‑1 (MQ=255)                                                                    ttCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGct                                                            <  1:414836/140‑1 (MQ=255)                                                                       cgccgcAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCtctc                                                         >  1:510318/1‑140 (MQ=255)                                                                             aGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAAc                                                   <  2:474861/140‑1 (MQ=255)                                                                              gTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACg                                                  <  2:798957/140‑1 (MQ=255)                                                                                         cGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTaa                                       >  2:749773/1‑140 (MQ=255)                                                                                           gCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGa                                     >  2:376567/1‑140 (MQ=255)                                                                                                ggATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACccac                                <  1:587552/140‑1 (MQ=255)                                                                                                ggATACGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACccac                                <  2:61766/140‑1 (MQ=255)                                                                                                    aCGAGACGAACACGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATc                            <  2:294681/140‑1 (MQ=255)                                                                                                                cGCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGc                <  1:64084/140‑1 (MQ=255)                                                                                                                 gCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCa               <  1:728684/140‑1 (MQ=255)                                                                                                                 gCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCa               <  2:65752/140‑1 (MQ=255)                                                                                                                 gCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCa               <  1:570303/140‑1 (MQ=255)                                                                                                                 gCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCa               <  2:571161/140‑1 (MQ=255)                                                                                                                 gCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCa               <  1:98433/140‑1 (MQ=255)                                                                                                                 gCCCTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCa               >  1:65752/1‑140 (MQ=255)                                                                                                                 gCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCa               <  1:474945/140‑1 (MQ=255)                                                                                                                  cccTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCAt              >  1:29464/1‑140 (MQ=255)                                                                                                                   ccTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATg             <  1:577755/140‑1 (MQ=255)                                                                                                                    cTCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAGGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATgg            <  1:46430/140‑1 (MQ=255)                                                                                                                     tCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGgt           <  2:510318/140‑1 (MQ=255)                                                                                                                     tCCGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGgt           >  2:205664/1‑140 (MQ=255)                                                                                                                       cGTCAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGgtgt         >  1:654275/1‑140 (MQ=255)                                                                                                                          cAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGa      <  2:654275/140‑1 (MQ=255)                                                                                                                          cAGAGGGAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGa      <  1:440791/140‑1 (MQ=255)                                                                                                                              gggAAACTCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATg  <  2:196271/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     CTGAAATAACCTCAGGCACCACAGACATACCAACAACATCCCCACCAATAATTTGCATCATGCGAATTTCCGCCGCAGTCTCGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATG  >  NC_000913/2524209‑2524473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑