Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,584,855	G→A	A224V (GCC→GTC)	aegA ←	putative oxidoreductase Fe‑S subunit AegA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,584,855	0	G	A	100.0%	101.0 / NA	30	A224V (GCC→GTC)	aegA	putative oxidoreductase Fe‑S subunit AegA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/10);  total (20/10)

GTCTGGTGAGAAAGCTCGACGGCGGCGTCGATGTTTCCGGCTTTCACCAGTTCAATCCACTGCGGTATATGGTTATGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGGCTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTACTCATTACC  >  NC_000913/2584723‑2584989                                                                                                                                     |                                                                                                                                       gTCTGGTGAGAAAGCTCGACGGCGGCGTCGATGTTTCCGGCTTTCACCAGTTCAATCCACTGCGGTATATGGTTATGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCg                                                                                                                                 <  1:109942/140‑1 (MQ=255)    tGGTGAGAAAGCTCGACGGCGGCGTCGATGTTTCCGGCTTTCACCAGTTCAATCCACTGCGGTATATGGTTATGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTtg                                                                                                                              >  2:801281/1‑140 (MQ=255)                tCGACGGCGGCGTCGATGTTTCCGGCTTTCACCAGTTCAATCCACTGCGGTATATGGTTATGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGgc                                                                                                                  >  2:737873/1‑140 (MQ=255)                   aCGGCGGCGTCGATGTTTCCGGCTTTCACCAGTTCAATCCACTGCGGTATATGGTTATGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGcgcg                                                                                                               >  1:648193/1‑140 (MQ=255)                                                                  tataTGGTTATGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCg                                                                <  2:648193/140‑1 (MQ=255)                                                                   atatGGTTATGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGc                                                               <  1:737873/140‑1 (MQ=255)                                                                         ttATGCAGCGGGCAGGACCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGttt                                                         >  1:456818/1‑140 (MQ=255)                                                                            tGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGc                                                               >  1:432763/1‑131 (MQ=255)                                                                            tGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGc                                                               <  2:432763/131‑1 (MQ=255)                                                                            tGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATc                                                      >  1:109457/1‑140 (MQ=255)                                                                            tGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATc                                                      >  1:450314/1‑140 (MQ=255)                                                                            tGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATc                                                      >  2:796329/1‑140 (MQ=255)                                                                            tGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATc                                                      >  1:477685/1‑140 (MQ=255)                                                                                 cGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCgg                                                   >  1:315125/1‑138 (MQ=255)                                                                                 cGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCgg                                                   <  2:315125/138‑1 (MQ=255)                                                                                  gggCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTc                                                >  1:640597/1‑140 (MQ=255)                                                                                   ggCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCg                                               >  1:793701/1‑140 (MQ=255)                                                                                        gTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCAcg                                          <  2:450314/140‑1 (MQ=255)                                                                                          ccATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCAcgcg                                        <  2:456818/140‑1 (MQ=255)                                                                                          ccATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCAcgcg                                        <  2:793701/140‑1 (MQ=255)                                                                                                       cTGTACTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCg                                  >  2:5507/1‑133 (MQ=255)                                                                                                       cTGTACTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCg                                  <  1:5507/133‑1 (MQ=255)                                                                                                        tgtgCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGc                          >  2:597163/1‑140 (MQ=255)                                                                                                          tgCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCAt                        >  2:154544/1‑140 (MQ=255)                                                                                                          tgCTCGCCGCACTTAAGGCAGCGCGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCAt                        >  1:454827/1‑140 (MQ=255)                                                                                                                              gcgcGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGtt                   >  2:178306/1‑125 (MQ=255)                                                                                                                              gcgcGAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGtt                   <  1:178306/125‑1 (MQ=255)                                                                                                                               cgcgAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGa                <  1:594356/127‑1 (MQ=255)                                                                                                                               cgcgAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGa                >  2:594356/1‑127 (MQ=255)                                                                                                                               cgcgAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTACTCATTAc   >  1:58240/1‑140 (MQ=255)                                                                                                                                gcgAGACTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTACTCATTAcc  >  1:114265/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       GTCTGGTGAGAAAGCTCGACGGCGGCGTCGATGTTTCCGGCTTTCACCAGTTCAATCCACTGCGGTATATGGTTATGCAGCGGGCAGGTCCATTCACAAACGCTGTGCTCGCCGCACTTAAGGCAGCGCGAGGCTTCCCGTTGTGCCTGGTCGGCGCGAAATGGCAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTACTCATTACC  >  NC_000913/2584723‑2584989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑