Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,200	T→C	A151A (GCA→GCG)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,200	0	T	C	100.0%	208.4 / NA	60	A151A (GCA→GCG)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/33);  total (27/33)

CGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGTGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCT  >  NC_000913/2638069‑2638333                                                                                                                                    |                                                                                                                                      cGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCAcc                                                                                                                               <  1:692786/140‑1 (MQ=255)     aaGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCcgcg                                                                                                                           >  2:420494/1‑140 (MQ=255)              aaGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGtt                                                                                                                  <  1:198645/140‑1 (MQ=255)                 gCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTtgc                                                                                                               <  2:621084/140‑1 (MQ=255)                  cATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTtgcc                                                                                                              >  2:372000/1‑140 (MQ=255)                    tatCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTtgccat                                                                                                            >  1:799518/1‑140 (MQ=255)                    tatCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTtgccat                                                                                                            >  2:506443/1‑140 (MQ=255)                      tCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTtgccatgc                                                                                                          <  1:92478/140‑1 (MQ=255)                         aGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCcac                                                                                                       <  1:420494/140‑1 (MQ=255)                          ggTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCcaca                                                                                                      <  2:799518/140‑1 (MQ=255)                          ggTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCcaca                                                                                                      <  1:506443/140‑1 (MQ=255)                           gTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGtt                                                                                                                  <  1:382005/127‑1 (MQ=255)                           gTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGtt                                                                                                                  >  2:382005/1‑127 (MQ=255)                              aaCTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTAc                                                                                                  >  2:534043/1‑140 (MQ=255)                               aCTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTtgcca                                                                                                             >  2:473757/1‑128 (MQ=255)                               aCTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTtgcca                                                                                                             <  1:473757/128‑1 (MQ=255)                                 tGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCAt                                                                                               <  1:777237/140‑1 (MQ=255)                                 tGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCAt                                                                                               <  1:484453/140‑1 (MQ=255)                                     cATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCt                                                                                           >  2:239183/1‑140 (MQ=255)                                     cATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCt                                                                                           >  1:172287/1‑140 (MQ=255)                                     cATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACCTTAGTTTGCCATGCCACAGTACCATCGCt                                                                                           >  1:553608/1‑140 (MQ=255)                                          gACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTAt                                                                                      >  2:669124/1‑140 (MQ=255)                                                gcCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCAt                                                                                               >  1:202688/1‑125 (MQ=255)                                                gcCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCAt                                                                                               <  2:202688/125‑1 (MQ=255)                                                  cGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGcgcg                                                                              >  2:269840/1‑140 (MQ=255)                                                   gTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGt                                                                             <  2:172287/140‑1 (MQ=255)                                                    tCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTa                                                                            >  2:229576/1‑140 (MQ=255)                                                                   gcTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTc                                                             <  2:305848/140‑1 (MQ=255)                                                                       gTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTg                                                         >  2:44635/1‑140 (MQ=255)                                                                       gTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTg                                                         <  1:375518/140‑1 (MQ=255)                                                                        tAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGc                                                        <  2:620846/140‑1 (MQ=255)                                                                        tAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGc                                                        <  1:239183/140‑1 (MQ=255)                                                                            tGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAAt                                                    >  2:99440/1‑140 (MQ=255)                                                                                       ggTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGACAGCGCTTCACCCGCGACCTTAGTTTGCCATGCCACAGTACCATCGCTGGTACTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGcc                                         <  2:553608/140‑1 (MQ=255)                                                                                            ggATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCccacca                                    <  1:269840/140‑1 (MQ=255)                                                                                             gATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCaa                                                     <  1:369872/122‑1 (MQ=255)                                                                                             gATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCaa                                                     >  2:369872/1‑122 (MQ=255)                                                                                              aTTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGa                                  <  1:99440/140‑1 (MQ=255)                                                                                              aTTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGa                                  <  1:44635/140‑1 (MQ=255)                                                                                                   cacCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATg                                           >  2:518236/1‑126 (MQ=255)                                                                                                   cacCAGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATg                                           <  1:518236/126‑1 (MQ=255)                                                                                                       aGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTcac                         >  2:134837/1‑140 (MQ=255)                                                                                                       aGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTcac                         >  1:328549/1‑140 (MQ=255)                                                                                                       aGACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTcac                         >  1:444240/1‑140 (MQ=255)                                                                                                        gACCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTcaca                        >  2:19589/1‑140 (MQ=255)                                                                                                         aCCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGa                                  <  1:467012/129‑1 (MQ=255)                                                                                                         aCCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGa                                  >  2:467012/1‑129 (MQ=255)                                                                                                         aCCGTCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCacac                       <  2:444240/140‑1 (MQ=255)                                                                                                             tCGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACAGTCACACCGcc                   >  1:386744/1‑140 (MQ=255)                                                                                                              cGCTGACCACCGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCa                  <  1:372000/140‑1 (MQ=255)                                                                                                                       ccGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAAt         >  1:88145/1‑140 (MQ=255)                                                                                                                        cGGGCGCGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATg        >  2:493817/1‑140 (MQ=255)                                                                                                                           gcgcGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAg     <  2:3686/140‑1 (MQ=255)                                                                                                                           gcgcGAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAg     <  1:288526/140‑1 (MQ=255)                                                                                                                            cgcgAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAgg    <  2:386744/140‑1 (MQ=255)                                                                                                                            cgcgAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAgg    <  1:534043/140‑1 (MQ=255)                                                                                                                            cgcgAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAgg    <  1:229576/140‑1 (MQ=255)                                                                                                                              cgAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGct  <  1:493817/140‑1 (MQ=255)                                                                                                                               gAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCa                  >  1:663253/1‑123 (MQ=255)                                                                                                                               gAAAGCGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCa                  <  2:663253/123‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      CGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTGGTGTGGATTAACACCAGACCGTCGCTGACCACCGGGCGCGAAAGTGCTTCACCCGCGACTTTAGTTTGCCATGCCACAGTACCATCGCTGGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCT  >  NC_000913/2638069‑2638333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑