Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,421	(A)7→6	coding (426/1644 nt)	yeeO ←	FMN/FAD exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,415	0	A	.	100.0%	257.4 / NA	60	coding (432/1644 nt)	yeeO	FMN/FAD exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (32/28);  total (32/28)

ATGAATAAGCGTTGCCAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGG  >  NC_000913/2059277‑2059549                                                                                                                                           |                                                                                                                                       atgaATAAGCGTTGCCAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCaa                                                                                                                                       <  2:734793/140‑1 (MQ=255)      tAAGCGTTGCCAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCaaaaaag                                                                                                                                  <  2:334504/140‑2 (MQ=255)          cGTTGCCAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCAt                                                                                                                             <  2:839422/140‑1 (MQ=255)               ccAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGa                                                                                                                        <  2:731051/140‑1 (MQ=255)               ccAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGa                                                                                                                        <  2:498531/140‑1 (MQ=255)               ccAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGa                                                                                                                        <  1:883718/140‑1 (MQ=255)                cAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGAc                                                                                                                       <  2:221666/140‑1 (MQ=255)                         ggCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGaa                                                                                                              >  1:12051/1‑140 (MQ=255)                           cAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAATATCGATAGCAGC‑AAAAAAGCCATAATGACCa                                                                                                                     <  1:337200/131‑1 (MQ=255)                           cAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAATATCGATAGCAGC‑AAAAAAGCCATAATGACCa                                                                                                                     >  2:337200/1‑131 (MQ=255)                              acaacGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGt                                                                                                         >  1:308051/1‑140 (MQ=255)                                            aCCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTg                                                                                                          >  1:945428/1‑125 (MQ=255)                                            aCCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTg                                                                                                          <  2:945428/125‑1 (MQ=255)                                            aCCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACg                                                                                           >  1:110109/1‑140 (MQ=255)                                              cAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGcc                                                                                         >  1:528430/1‑140 (MQ=255)                                                aTGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAGGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCgg                                                                                       <  1:640766/140‑1 (MQ=255)                                                aTGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCgg                                                                                       <  1:353436/140‑1 (MQ=255)                                                 tGACTGCCGCGTCGCCACCCTCGCTCG‑CGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGcc                                                                                     >  1:314389/1‑140 (MQ=255)                                                   aCTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCa                                                                                    >  1:939081/1‑140 (MQ=255)                                                     tGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATc                                                                                  >  2:641868/1‑140 (MQ=255)                                                       ccGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGc                                                                                >  2:785414/1‑140 (MQ=255)                                                          cgTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATc                                                                             <  1:134130/140‑1 (MQ=255)                                                                      cgctcgTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCAt                                                                                   >  2:558342/1‑122 (MQ=255)                                                                      cgctcgTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCAt                                                                                   <  1:558342/122‑1 (MQ=255)                                                                      cgctcgTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCccagcca                                                                 >  1:730175/1‑140 (MQ=255)                                                                      cgctcgTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCccagcca                                                                 >  2:344174/1‑140 (MQ=255)                                                                      cgctcgTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCccagcca                                                                 >  2:811252/1‑140 (MQ=255)                                                                      cgctcgTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCccagcca                                                                 >  2:706572/1‑140 (MQ=255)                                                                       gctcgTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCccagccag                                                                <  1:494104/140‑1 (MQ=255)                                                                         tcgtcgtcgATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCt                                                              <  2:362743/140‑1 (MQ=255)                                                                                     ccGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGt                                                  <  2:12051/140‑1 (MQ=255)                                                                                      cGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTg                                                 >  1:99256/1‑140 (MQ=255)                                                                                          tACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCa                                             <  1:344174/140‑1 (MQ=255)                                                                                             cGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGaa                                          >  1:621974/1‑140 (MQ=255)                                                                                                 aCTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAAcccc                                      >  1:491416/1‑140 (MQ=255)                                                                                                 aCTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAAcccc                                      >  1:127741/1‑140 (MQ=255)                                                                                                  cTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAAc                                         >  1:409087/1‑136 (MQ=255)                                                                                                  cTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAAc                                         <  2:409087/136‑1 (MQ=255)                                                                                                          cacaACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAg                             >  2:191931/1‑140 (MQ=255)                                                                                                          cacaACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAg                             >  2:860506/1‑140 (MQ=255)                                                                                                           acaACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAg                             <  1:404104/139‑1 (MQ=255)                                                                                                           acaACGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAg                             >  2:404104/1‑139 (MQ=255)                                                                                                              aCGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGaca                         <  2:127741/140‑1 (MQ=255)                                                                                                              aCGACAGTAGTACCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGaca                         <  1:811252/140‑1 (MQ=255)                                                                                                              aCGACAGTAGTACCAAGATCGACAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGaca                         <  1:706572/140‑1 (MQ=255)                                                                                                                        taCCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtct               <  2:528430/140‑1 (MQ=255)                                                                                                                         aCCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc              >  2:948726/1‑140 (MQ=255)                                                                                                                         aCCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc              <  2:621974/140‑1 (MQ=255)                                                                                                                         aCCAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAAT‑CCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTcc             >  2:589347/1‑140 (MQ=255)                                                                                                                          ccAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTcc             <  2:152847/140‑1 (MQ=255)                                                                                                                           cAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCa            <  2:314389/140‑1 (MQ=255)                                                                                                                           cAAGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCa            <  2:730175/140‑1 (MQ=255)                                                                                                                             aGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATg          >  2:995809/1‑140 (MQ=255)                                                                                                                             aGATCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATg          >  1:598771/1‑140 (MQ=255)                                                                                                                               aTCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCGACAGGACACAGGCATTCTCCATGaa        >  1:67726/1‑140 (MQ=255)                                                                                                                               aTCGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGaa        >  1:879596/1‑140 (MQ=255)                                                                                                                                 cGATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGa      >  1:399490/1‑140 (MQ=255)                                                                                                                                  gATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGAt     >  2:115295/1‑140 (MQ=255)                                                                                                                                  gATAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGAt     >  1:956089/1‑140 (MQ=255)                                                                                                                                    tAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAg   <  2:598771/140‑1 (MQ=255)                                                                                                                                    tAGCAGC‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAAT‑CCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAgg  <  1:589347/140‑1 (MQ=255)                                                                                                                                     agcagc‑AAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAgg  <  1:785414/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       ATGAATAAGCGTTGCCAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGG  >  NC_000913/2059277‑2059549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑