Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,573	(C)6→5	intergenic (+52/‑95)	yegP → / → yegQ	DUF1508 domain‑containing protein YegP/putative peptidase YegQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,568	0	C	.	100.0%	124.9 / NA	30	intergenic (+47/‑100)	yegP/yegQ	DUF1508 domain‑containing protein YegP/putative peptidase YegQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/17);  total (13/17)

AGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCC  >  NC_000913/2165429‑2165691                                                                                                                                            |                                                                                                                            agccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACTATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGc                                                                                                                             >  1:545422/1‑140 (MQ=255) agccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGc                                                                                                                             <  2:437544/140‑1 (MQ=255) agccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGc                                                                                                                             >  1:579582/1‑140 (MQ=255)   ccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGccc                                                                                                                           >  2:863256/1‑140 (MQ=255)      gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGccccca                                                                                                                        <  1:145514/140‑2 (MQ=255)      gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGccccca                                                                                                                        <  2:785402/140‑2 (MQ=255)      gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGccccca                                                                                                                        <  1:345453/140‑2 (MQ=255)       aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGcccccac                                                                                                                       <  1:863256/140‑3 (MQ=255)                 ccGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGACAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGccccca                                                                                                                        <  1:873234/129‑2 (MQ=255)                 ccGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGccccca                                                                                                                        >  2:873234/1‑128 (MQ=255)                    cgcAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATgc                                                                                                         >  1:935063/1‑140 (MQ=255)                    cgcAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATgc                                                                                                         >  2:308897/1‑140 (MQ=255)                     gcAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATgcc                                                                                                        <  1:290190/140‑1 (MQ=255)                     gcAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATgcc                                                                                                        <  1:560406/140‑1 (MQ=255)                       aaTCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATgcccg                                                                                                      <  2:720523/140‑1 (MQ=255)                                     ggAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTgggg                                                                                        >  1:456602/1‑140 (MQ=255)                                     ggAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTgggg                                                                                        >  1:706123/1‑140 (MQ=255)                                        aTTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACTATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCa                                                                                     <  2:545422/140‑1 (MQ=255)                                              tCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTcccc                                                                               >  2:773357/1‑140 (MQ=255)                                               ccGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCt                                                                              <  1:308897/140‑1 (MQ=255)                                                      aGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCt                                                                       >  1:451157/1‑140 (MQ=255)                                                          aaTGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtcat                                                                   >  1:116986/1‑140 (MQ=255)                                                          aaTGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtcat                                                                   <  2:451157/140‑1 (MQ=255)                                                          aaTGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtcat                                                                   >  2:954774/1‑140 (MQ=255)                                                                     ccAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCg                                                        <  1:954774/140‑1 (MQ=255)                                                                            gTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGa                                                 <  1:94065/140‑1 (MQ=255)                                                                             tGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAc                                                <  2:386503/140‑1 (MQ=255)                                                                             tGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAc                                                <  2:706123/140‑1 (MQ=255)                                                                                   aCAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtca                                          >  1:258103/1‑140 (MQ=255)                                                                                           tAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACg                                  <  1:773357/140‑1 (MQ=255)                                                                                            aaTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACga                                 <  2:935063/140‑1 (MQ=255)                                                                                            aaTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACga                                 <  2:711957/140‑1 (MQ=255)                                                                                                   aTGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAtt                          >  2:31781/1‑140 (MQ=255)                                                                                                           cGTGGGTTGTGGGCGTCCGGCGCTTTACCAAAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTaa                  <  2:614208/140‑1 (MQ=255)                                                                                                             tGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAAc                <  2:992514/140‑1 (MQ=255)                                                                                                             tGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAAc                <  2:116986/140‑1 (MQ=255)                                                                                                                    ttgGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAt                           <  2:261301/122‑1 (MQ=255)                                                                                                                    ttgGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAt                           >  1:261301/1‑122 (MQ=255)                                                                                                                    ctgGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACt         >  1:161023/2‑140 (MQ=255)                                                                                                                           ccGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTcc  >  2:401934/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                            AGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCC  >  NC_000913/2165429‑2165691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑