Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,201	(T)6→5	coding (256/1278 nt)	yggP ←	putative zinc‑binding dehydrogenase YggP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,196	0	T	.	100.0%	235.4 / NA	55	coding (261/1278 nt)	yggP	putative zinc‑binding dehydrogenase YggP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (25/30);  total (25/30)

GGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAACGCCGCTTTCCAG  >  NC_000913/3077059‑3077331                                                                                                                                          |                                                                                                                                        ggTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCttt                                                                                                                                       <  2:869760/140‑1 (MQ=255)   tAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCttttt                                                                                                                                     >  2:618954/1‑140 (MQ=255)           aGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGc                                                                                                                            <  1:300527/140‑1 (MQ=255)             cccAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCttt                                                                                                                                       >  2:408203/1‑128 (MQ=255)             cccAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCttt                                                                                                                                       <  1:408203/128‑1 (MQ=255)             cccAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCa                                                                                                                          >  2:111450/1‑140 (MQ=255)                         gCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTa                                                                                                              <  1:111450/140‑1 (MQ=255)                         gCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTa                                                                                                              <  1:449939/140‑1 (MQ=255)                           gATTTCGGGAATAATCATATAAGTGGCATTACCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTAcc                                                                                                            <  2:611617/140‑1 (MQ=255)                            aTTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTAccc                                                                                                           >  1:620056/1‑140 (MQ=255)                             tttCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCa                                                                                                          >  1:71673/1‑140 (MQ=255)                                cGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACtt                                                                                                       <  1:725650/140‑1 (MQ=255)                                       aatCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAAt                                                                                                <  2:969485/140‑1 (MQ=255)                                              taAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCg                                                                                         <  2:951266/140‑1 (MQ=255)                                              taAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGCGAGATTTTTACCCACTTCGACAATAACCCCg                                                                                         <  2:393895/140‑1 (MQ=255)                                                aGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGc                                                                                       <  1:618954/140‑1 (MQ=255)                                                 gTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGca                                                                                      <  2:71673/140‑1 (MQ=255)                                                 gTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGca                                                                                      >  2:806981/1‑140 (MQ=255)                                                 gTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGca                                                                                      <  2:248071/140‑1 (MQ=255)                                                  tGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCg                                                                                                     <  1:400475/124‑1 (MQ=255)                                                  tGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCg                                                                                                     >  2:400475/1‑124 (MQ=255)                                                  tGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAccc                                                                                           >  2:986580/1‑134 (MQ=255)                                                  tGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAccc                                                                                           <  1:986580/134‑1 (MQ=255)                                                   ggCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGcaca                                                                                    >  1:751434/1‑140 (MQ=255)                                                       ttGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCa                                                                                >  1:970999/1‑140 (MQ=255)                                                       ttGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCa                                                                                >  1:396625/1‑140 (MQ=255)                                                         gccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCgg                                                                                        <  1:179355/130‑1 (MQ=255)                                                         gccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCgg                                                                                        >  2:179355/1‑130 (MQ=255)                                                          ccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGc                                                                             >  1:267011/1‑140 (MQ=255)                                                          ccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGc                                                                             <  1:277478/140‑1 (MQ=255)                                                           cgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGcc                                                                            <  1:970811/140‑1 (MQ=255)                                                                           gCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGgatg                                                            >  1:50847/1‑140 (MQ=255)                                                                             tGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGgatgat                                                          >  2:280192/1‑140 (MQ=255)                                                                                 gCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTc                                                      >  1:748765/1‑140 (MQ=255)                                                                                    cGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTaa                                                   >  1:717567/1‑140 (MQ=255)                                                                                      cTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGAc                                                                  <  2:601606/123‑1 (MQ=255)                                                                                      cTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGAc                                                                  >  1:601606/1‑123 (MQ=255)                                                                                      cTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTACCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAAt                                                 >  1:565754/1‑140 (MQ=255)                                                                                        gAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAAtcg                                               <  2:620056/140‑1 (MQ=255)                                                                                                 cTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTAcg                                      >  1:349857/1‑140 (MQ=255)                                                                                                   tGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTAcgcg                                    <  2:267011/140‑1 (MQ=255)                                                                                                   tGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTAcgcg                                    >  2:113049/1‑140 (MQ=255)                                                                                                      tAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGttt                                 <  2:224847/140‑1 (MQ=255)                                                                                                      tAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGttt                                 <  1:280192/140‑1 (MQ=255)                                                                                                      tAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGttt                                 <  2:751434/140‑1 (MQ=255)                                                                                                           ccATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGtt                            <  1:113049/140‑1 (MQ=255)                                                                                                           ccATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTACGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGtt                            <  1:690552/140‑1 (MQ=255)                                                                                                            cATCGCCGGTTGCAATACAAAACGCTTGCC‑TTTTTATATTTGCCAGTAAGCTTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGATCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTc                           >  2:649615/1‑140 (MQ=255)                                                                                                             aTCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCa                          <  1:806981/140‑1 (MQ=255)                                                                                                              tCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCAc                         >  2:200072/1‑140 (MQ=255)                                                                                                                    gTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGa                   >  2:399860/1‑140 (MQ=255)                                                                                                                    gTTGCAATACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGa                   >  1:188567/1‑140 (MQ=255)                                                                                                                           tACAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTAGGGTACGCGTTTATGTTCACTACCGAGTAAcgc            <  2:748765/140‑1 (MQ=255)                                                                                                                             cAAAACGCTGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAAcgccg          <  1:695483/140‑1 (MQ=255)                                                                                                                                    cTGGCC‑TTTTTATATTTACCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAACGCCGCTTTCCa   <  2:565754/140‑1 (MQ=255)                                                                                                                                     tGGCC‑TTTTTATATTTTCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAAcgccg          <  2:414413/132‑1 (MQ=255)                                                                                                                                     tGGCC‑TTTTTATATTTTCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAAcgccg          >  1:414413/1‑132 (MQ=255)                                                                                                                                     tGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAACGCCGCTTTCCAg  <  2:717567/140‑1 (MQ=255)                                                                                                                                     tGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAACGCCGCTTTCCAg  >  1:638415/1‑140 (MQ=255)                                                                                                                                     tGGCC‑TTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAACGCCGCTTTCCAg  >  2:879856/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTTATGTTCACTACCGAGTAACGCCGCTTTCCAG  >  NC_000913/3077059‑3077331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑