Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,156,525	(T)5→4	intergenic (+7/‑98)	yqhD → / → dkgA	NADPH‑dependent aldehyde reductase YqhD/methylglyoxal reductase DkgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,156,521	0	T	.	100.0%	151.3 / NA	36	intergenic (+3/‑102)	yqhD/dkgA	NADPH‑dependent aldehyde reductase YqhD/methylglyoxal reductase DkgA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/24);  total (12/24)

GACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTA  >  NC_000913/3156387‑3156649                                                                                                                                       |                                                                                                                                 gACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCttttac                                                                                                                             >  2:243102/1‑138 (MQ=255)  aCTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCttttacg                                                                                                                            >  2:267515/1‑137 (MQ=255)            ggACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGCCCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACtt                                                                                                                 <  2:393732/140‑1 (MQ=255)             gACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTc                                                                                                                       <  2:892613/133‑1 (MQ=255)             gACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTc                                                                                                                       >  1:892613/1‑133 (MQ=255)                  cAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGttt                                                                                                           >  1:932446/1‑140 (MQ=255)                      tCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCgg                                                                                                       <  2:130716/140‑1 (MQ=255)                      tCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCgg                                                                                                       <  1:974741/140‑1 (MQ=255)                        cATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGc                                                                                                     <  2:527979/140‑1 (MQ=255)                               gCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACACCTCAAACTTTCGTTTTCGGGCATTTCGt                                                                                              <  2:708055/140‑1 (MQ=255)                                  ttGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCa                                                                                           <  1:37713/140‑1 (MQ=255)                                    gCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGa                                                                                         >  2:154351/1‑140 (MQ=255)                                       gAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACtt                                                                                      <  2:545167/140‑1 (MQ=255)                                         aaaaaCTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTaa                                                                                    <  2:344947/140‑1 (MQ=255)                                            aaCTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGtt                                                                                 <  1:267515/140‑1 (MQ=255)                                               tGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTcac                                                                              >  2:594419/1‑140 (MQ=255)                                                       cACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTc                                                                      >  1:761137/1‑140 (MQ=255)                                                          ggCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCAcc                                                                   <  1:354099/140‑1 (MQ=255)                                                          ggCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCAcc                                                                   <  2:597473/140‑1 (MQ=255)                                                                       tGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCg                                                      <  1:876428/140‑1 (MQ=255)                                                                       tGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCg                                                      <  2:731953/140‑1 (MQ=255)                                                                        gggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCgg                                                     <  1:96685/140‑1 (MQ=255)                                                                        gggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCgg                                                     <  1:73044/140‑1 (MQ=255)                                                                         ggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGt                                                    >  2:613938/1‑140 (MQ=255)                                                                         ggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGt                                                    <  2:459991/140‑1 (MQ=255)                                                                             aaaaTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGt                                                <  2:761137/140‑1 (MQ=255)                                                                             aaaaTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGt                                                <  1:154351/140‑1 (MQ=255)                                                                                          tACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAaggag                                   <  2:773499/140‑1 (MQ=255)                                                                                           aCGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAaggagg                                  <  2:932446/140‑1 (MQ=255)                                                                                             gTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGaa                                >  2:768761/1‑140 (MQ=255)                                                                                             gTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGaa                                >  2:801599/1‑140 (MQ=255)                                                                                                                    aCGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttat         <  1:819664/140‑1 (MQ=255)                                                                                                                    aCGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttat         <  1:594419/140‑1 (MQ=255)                                                                                                                     cGAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt        <  1:243102/140‑1 (MQ=255)                                                                                                                      gAAGCCGCCCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatta       >  2:47290/1‑140 (MQ=255)                                                                                                                         gccgccCGCTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGc    >  2:666846/1‑140 (MQ=255)                                                                                                                           cgcccgcTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTa  >  2:833818/1‑140 (MQ=255)                                                                                                                           cgcccgcTAAGC‑TTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTa  >  1:494176/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                 GACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTA  >  NC_000913/3156387‑3156649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑