Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,440	(T)6→7	coding (223/1632 nt)	dcuS ←	sensory histidine kinase DcuS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,434	1	.	T	100.0%	313.7 / NA	89	P77T (CCG→ACG)	dcuS	sensory histidine kinase DcuS
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (49/40);  total (49/40)

CCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGG‑TTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATA  >  NC_000913/4351298‑4351565                                                                                                                                          |                                                                                                                                    ccTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGG‑ttt                                                                                                                                  >  1:451967/1‑140 (MQ=255)  cTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGG‑tttt                                                                                                                                 >  2:388157/1‑140 (MQ=255)    ttAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGG‑tttttt                                                                                                                               >  1:988127/1‑140 (MQ=255)     tAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGG‑ttttttt                                                                                                                              >  2:603311/1‑139 (MQ=255)       aaTGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGG‑tttttttct                                                                                                                            >  1:862895/1‑137 (MQ=255)        aTGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACTATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGG‑tttttttctg                                                                                                                           <  2:797013/140‑5 (MQ=255)          ggCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCa                                                                                                                          <  2:988127/140‑1 (MQ=255)            cTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAg                                                                                                                        >  1:450632/1‑140 (MQ=255)            cTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAg                                                                                                                        >  1:348102/1‑140 (MQ=255)               aCCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCTCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGccc                                                                                                                     >  2:229659/1‑140 (MQ=255)                      cGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGa                                                                                                              >  2:708083/1‑140 (MQ=255)                         tGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGAttt                                                                                                           >  1:818035/1‑140 (MQ=255)                           ggCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTcc                                                                                                         >  2:271233/1‑140 (MQ=255)                           ggCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTcc                                                                                                         >  1:854833/1‑140 (MQ=255)                                cAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGccc                                                                                                                     <  2:872097/123‑1 (MQ=255)                                cAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGAGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGccc                                                                                                                     >  1:872097/1‑123 (MQ=255)                                 aGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAg                                                                                                   <  1:946123/140‑1 (MQ=255)                                     tGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCgg                                                                                               >  2:249479/1‑140 (MQ=255)                                     tGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCgg                                                                                               >  2:420652/1‑140 (MQ=255)                                        gAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGa                                                                                            <  2:596930/140‑1 (MQ=255)                                        gAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGa                                                                                            <  2:818035/140‑1 (MQ=255)                                        gAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGa                                                                                            >  2:838017/1‑140 (MQ=255)                                            aGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGt                                                                                        <  1:304094/140‑1 (MQ=255)                                             gCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTa                                                                                       >  2:322443/1‑140 (MQ=255)                                             gCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTa                                                                                       >  1:241719/1‑140 (MQ=255)                                             gCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTa                                                                                       >  2:100560/1‑140 (MQ=255)                                             gCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTa                                                                                       <  1:388157/140‑1 (MQ=255)                                              cGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTAc                                                                                      <  1:10033/140‑1 (MQ=255)                                                    cTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCa                                                                                >  2:354904/1‑140 (MQ=255)                                                      ttGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACt                                                                              >  1:537179/1‑140 (MQ=255)                                                       tGCATATCGGTAGCGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTg                                                                             >  1:375949/1‑140 (MQ=255)                                                       tGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTg                                                                             >  2:54940/1‑140 (MQ=255)                                                             tCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTg                                                                       >  1:240753/1‑140 (MQ=255)                                                             tCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTg                                                                       >  2:401926/1‑140 (MQ=255)                                                              cGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGc                                                                      >  2:726874/1‑140 (MQ=255)                                                              cGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGc                                                                      >  1:457463/1‑140 (MQ=255)                                                               ggTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGcc                                                                     >  2:195758/1‑140 (MQ=255)                                                                 tAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCtt                                                                   >  2:755950/1‑140 (MQ=255)                                                                 tAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCtt                                                                   <  2:457463/140‑1 (MQ=255)                                                                 tAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGCCGGCGAGGGTACGCGCCACTGCCAGTGCCtt                                                                   >  2:367451/1‑140 (MQ=255)                                                                  aaCGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttg                                                                  <  1:708083/140‑1 (MQ=255)                                                                  aaCGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttg                                                                  <  1:420652/140‑1 (MQ=255)                                                                   aCGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttgt                                                                 >  1:279227/1‑140 (MQ=255)                                                                     gACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttgttg                                                               <  1:838017/140‑1 (MQ=255)                                                                      aCAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTgg                                                              <  2:241719/140‑1 (MQ=255)                                                                      aCAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTgg                                                              <  1:354904/140‑1 (MQ=255)                                                                      aCAATAAACAGCAGATCGTTGCGTTTGCGTACGGCCTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTgg                                                              <  1:634775/140‑1 (MQ=255)                                                                       cAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGc                                                             <  1:755950/140‑1 (MQ=255)                                                                        aaTAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCt                                                            >  1:122467/1‑140 (MQ=255)                                                                          tAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAg                                                          >  1:1001281/1‑140 (MQ=255)                                                                          tAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAg                                                          >  1:846831/1‑140 (MQ=255)                                                                           aaaCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGc                                                         <  1:249479/140‑1 (MQ=255)                                                                           aaaCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGc                                                         <  2:846831/140‑1 (MQ=255)                                                                            aaCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGcc                                                        <  2:348102/140‑1 (MQ=255)                                                                               agcagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAt                                                     <  1:726874/140‑1 (MQ=255)                                                                               agcagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAt                                                     <  1:755263/140‑1 (MQ=255)                                                                                gcagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtc                                                    >  1:912120/1‑140 (MQ=255)                                                                                 cagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtct                                                   <  2:558304/140‑1 (MQ=255)                                                                                 cagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtct                                                   <  1:28585/140‑1 (MQ=255)                                                                                 cagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtct                                                   >  1:821921/1‑140 (MQ=255)                                                                                  agATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtctc                                                  >  2:811227/1‑140 (MQ=255)                                                                                  agATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtctc                                                  >  2:904026/1‑140 (MQ=255)                                                                                   gATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCATTGCCTTGTTGGCTAGCCCATCTcg                                                 <  2:854833/140‑1 (MQ=255)                                                                                   gATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTcg                                                 <  2:537179/140‑1 (MQ=255)                                                                                   gATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTcg                                                 <  1:777318/140‑1 (MQ=255)                                                                                    aTCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTTCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTgg                                                              <  1:903891/126‑1 (MQ=255)                                                                                    aTCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTTCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTgg                                                              >  2:903891/1‑126 (MQ=255)                                                                                    aTCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTcgc                                                >  2:502233/1‑140 (MQ=255)                                                                                     tCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTcgcg                                               <  2:450632/140‑1 (MQ=255)                                                                                     tCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTcgcg                                               <  2:240753/140‑1 (MQ=255)                                                                                      cGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGt                                              >  1:640110/1‑140 (MQ=255)                                                                                                     cGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGAGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTg                               >  2:562808/1‑140 (MQ=255)                                                                                                         ttCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCgag                           <  1:322443/140‑1 (MQ=255)                                                                                                         ttCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCgag                           <  2:122467/140‑1 (MQ=255)                                                                                                           ccGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGaa                         >  1:879795/1‑140 (MQ=255)                                                                                                           ccGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGaa                         >  1:117236/1‑140 (MQ=255)                                                                                                                aTGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCgaga                          >  1:969089/1‑134 (MQ=255)                                                                                                                aTGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCgaga                          <  2:969089/134‑1 (MQ=255)                                                                                                                aTGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTaaa                    <  1:271233/140‑1 (MQ=255)                                                                                                                aTGGCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTaaa                    >  2:365412/1‑140 (MQ=255)                                                                                                                  ggCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATc                  <  1:229659/140‑1 (MQ=255)                                                                                                                  ggCCTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCCAGTCGGCCACGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATc                  >  2:755637/1‑140 (MQ=255)                                                                                                                    ccTGGATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAg                <  1:100560/140‑1 (MQ=255)                                                                                                                       ggATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATg             <  1:133690/140‑1 (MQ=255)                                                                                                                        gATGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGc            <  1:502233/140‑1 (MQ=255)                                                                                                                         aTGCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGca           <  2:879795/140‑1 (MQ=255)                                                                                                                           gCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGcacc         <  2:44990/140‑1 (MQ=255)                                                                                                                           gCCACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGcacc         >  1:43980/1‑140 (MQ=255)                                                                                                                             cACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGcaccac       >  2:644924/1‑140 (MQ=255)                                                                                                                             cACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGcaccac       >  2:348302/1‑140 (MQ=255)                                                                                                                             cACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGcaccac       >  1:663160/1‑140 (MQ=255)                                                                                                                             cACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGcacc         >  1:98127/1‑138 (MQ=255)                                                                                                                             cACTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGcacc         <  2:98127/138‑1 (MQ=255)                                                                                                                              aCTCTCCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCaccacc      >  1:470266/1‑140 (MQ=255)                                                                                                                                tctcCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCaa    >  1:38409/1‑140 (MQ=255)                                                                                                                                  tcCTGCGGTTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATa  >  1:105691/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    CCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGG‑TTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATA  >  NC_000913/4351298‑4351565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑