Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,842	T→C	G502G (GGT→GGC)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,842	0	T	C	100.0%	204.6 / NA	58	G502G (GGT→GGC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/29);  total (29/29)

ATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCT  >  NC_000913/1708‑1976                                                                                                                                       |                                                                                                                                       aTGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGc                                                                                                                                   >  1:545442/1‑140 (MQ=255) aTGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGc                                                                                                                                   <  1:531680/140‑1 (MQ=255) aTGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGc                                                                                                                                   >  2:208629/1‑140 (MQ=255)  tGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGcc                                                                                                                                  <  2:361504/140‑1 (MQ=255)       ttCAATACCGCTCACGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTc                                                                                                                             <  2:865620/140‑1 (MQ=255)       ttCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTc                                                                                                                             >  1:126124/1‑140 (MQ=255)            tACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAgg                                                                                                                        <  1:208629/140‑1 (MQ=255)            tACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAgg                                                                                                                        <  2:425246/140‑1 (MQ=255)               cGATCAGGTTATCGAAGTGTTTGTGATTGGCGCCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGctc                                                                                                                     <  1:918230/140‑1 (MQ=255)                 aTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGct                                                                                                                      <  2:789157/137‑1 (MQ=255)                 aTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGct                                                                                                                      >  1:789157/1‑137 (MQ=255)                 aTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTg                                                                                                                   >  1:464512/1‑140 (MQ=255)                 aTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTg                                                                                                                   >  2:837603/1‑140 (MQ=255)                 aTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTg                                                                                                                   >  1:698498/1‑140 (MQ=255)                 aTCAGGTTATCGAAGTGTTTGTGATTGGAGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTg                                                                                                                   >  1:815078/1‑140 (MQ=255)                   cAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCt                                                                                                                 <  2:936665/140‑1 (MQ=255)                   cAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCt                                                                                                                 <  2:93862/140‑1 (MQ=255)                       ttATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCAcc                                                                                                             <  2:466885/140‑1 (MQ=255)                       ttATCGAAGTGTTTGTGATTGGAGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCAcc                                                                                                             <  2:815078/140‑1 (MQ=255)                         aTCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCt                                                                                                                 <  2:261638/134‑1 (MQ=255)                         aTCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCt                                                                                                                 >  1:261638/1‑134 (MQ=255)                             aaGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTa                                                                                                       >  1:74198/1‑140 (MQ=255)                              aGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTAc                                                                                                      >  2:115540/1‑140 (MQ=255)                                tgtTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACAt                                                                                                    >  1:92360/1‑140 (MQ=255)                                          tGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTTCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATc                                                                                          >  1:76412/1‑140 (MQ=255)                                               tCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGaa                                                                                     <  2:464512/140‑1 (MQ=255)                                               tCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGaa                                                                                     >  1:3741/1‑140 (MQ=255)                                                       gTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCa                                                                             <  1:644175/140‑1 (MQ=255)                                                               tGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAAc                                                                     <  2:3741/140‑1 (MQ=255)                                                                    tgctgGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGgcg                                                                >  2:719012/1‑140 (MQ=255)                                                                      ctgGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCa                                                              <  1:684538/140‑1 (MQ=255)                                                                           gCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCa                                                         >  2:89431/1‑140 (MQ=255)                                                                              aCTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAag                                                      >  2:406607/1‑140 (MQ=255)                                                                                tGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAACCAAagag                                                    <  1:837603/140‑1 (MQ=255)                                                                                    gCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAagag                                                    >  1:160022/1‑136 (MQ=255)                                                                                    gCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAagag                                                    <  2:160022/136‑1 (MQ=255)                                                                                      gTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGttt                                              <  1:745033/140‑1 (MQ=255)                                                                                       tCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTa                                             >  1:890141/1‑140 (MQ=255)                                                                                         aGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAt                                           >  2:351337/1‑140 (MQ=255)                                                                                         aGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAt                                           >  1:797288/1‑140 (MQ=255)                                                                                              aGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCgg                                      <  1:719012/140‑1 (MQ=255)                                                                                                                  tatCGACTTACGTGTCTTCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGa                  <  2:76412/140‑1 (MQ=255)                                                                                                                    tCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCtt                                <  1:59879/124‑1 (MQ=255)                                                                                                                    tCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCtt                                >  2:59879/1‑124 (MQ=255)                                                                                                                    tCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGaaa                >  2:656260/1‑140 (MQ=255)                                                                                                                    tCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGaaa                >  1:422645/1‑140 (MQ=255)                                                                                                                    tCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGaa                 <  2:503581/139‑1 (MQ=255)                                                                                                                    tCGACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGaa                 >  1:503581/1‑139 (MQ=255)                                                                                                                      gACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGa              <  2:276207/140‑1 (MQ=255)                                                                                                                      gACTTACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGa              <  2:241036/140‑1 (MQ=255)                                                                                                                          tACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTg                   <  1:765743/131‑1 (MQ=255)                                                                                                                          tACGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTg                   >  2:765743/1‑131 (MQ=255)                                                                                                                           aCGTGTCTGCGGCGTTGTCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGCACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatc         <  2:890141/140‑1 (MQ=255)                                                                                                                           aCGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatc         >  2:466077/1‑140 (MQ=255)                                                                                                                           aCGTGTCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatc         >  1:954890/1‑140 (MQ=255)                                                                                                                             gtgtCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatcat       <  2:422645/140‑1 (MQ=255)                                                                                                                             gtgtCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatcat       <  1:351337/140‑1 (MQ=255)                                                                                                                              tgtCTGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatcatc      >  1:656752/1‑140 (MQ=255)                                                                                                                                  tGCGGCGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATctgct  >  1:838186/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       ATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCT  >  NC_000913/1708‑1976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑