Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,211,964	T→C	G286G (GGT→GGC)	dnaG →	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,211,964	0	T	C	100.0%	176.4 / NA	54	G286G (GGT→GGC)	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/24);  total (30/24)

AAGGCCGCCAGCTTTACGGTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGTACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACA  >  NC_000913/3211828‑3212091                                                                                                                                         |                                                                                                                                aaGGCCGCCAGCTTTACGGTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACg                                                                                                                              >  2:559412/1‑140 (MQ=255)       gccAGCTTTACGGTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAacc                                                                                                                        >  2:204831/1‑140 (MQ=255)              ttACGGTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCAccgccg                                                                                                                 >  1:49302/1‑140 (MQ=255)               tACGGTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGa                                                                                                                <  1:625034/140‑1 (MQ=255)               tACGGTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGa                                                                                                                >  2:890755/1‑140 (MQ=255)                  ggTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATca                                                                                                             <  2:718428/140‑1 (MQ=255)                      tttATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGa                                                                                                                <  2:228092/133‑1 (MQ=255)                      tttATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGa                                                                                                                >  1:228092/1‑133 (MQ=255)                       ttATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCAccgc                                                                                                                   <  2:858420/129‑1 (MQ=255)                       ttATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCAccgc                                                                                                                   >  1:858420/1‑129 (MQ=255)                       ttATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATAc                                                                                                        <  1:531971/140‑1 (MQ=255)                       ttATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATAc                                                                                                        >  1:975559/1‑140 (MQ=255)                       ttATGAAGCGCAGCAGGATAACGCTGAACCCAACGTTCGGCTTGTGGTAAAAGGCTATATGGACGTGGGGGCGCGGCCGAAATCCGGCATTAATTACCCCGTGCCTTCTTTAGGCACGTCAACCACCGCCGATCACATAc                                                                                                        <  2:917005/140‑1 (MQ=255)                         aTGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACaa                                                                                                      <  2:825539/140‑1 (MQ=255)                              gcgcAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATAc                                                                                                        <  2:983934/133‑1 (MQ=255)                              gcgcAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATAc                                                                                                        >  1:983934/1‑133 (MQ=255)                              gcgcAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACtgtt                                                                                                 >  2:371193/1‑140 (MQ=255)                                gcagcaGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACtgttgt                                                                                               >  2:747161/1‑140 (MQ=255)                                gcagcaGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACtgttgt                                                                                               >  2:344856/1‑140 (MQ=255)                                  agcagGATAACGCTGAACCTAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTc                                                                                             >  2:518187/1‑140 (MQ=255)                                       gATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGACAACCACCGCCGATCACATACAACTGTTGTTCcgcgc                                                                                        <  2:882119/140‑1 (MQ=255)                                          aaCGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGAc                                                                                     <  1:890755/140‑1 (MQ=255)                                          aaCGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGAc                                                                                     <  1:747161/140‑1 (MQ=255)                                            cGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACca                                                                                   >  2:313522/1‑140 (MQ=255)                                               tGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACcaaca                                                                                <  1:204831/140‑1 (MQ=255)                                                 aaCCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCcgc                                                                                          >  2:512758/1‑128 (MQ=255)                                                 aaCCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCcgc                                                                                          <  1:512758/128‑1 (MQ=255)                                                 aaCCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAAt                                                                              <  1:371193/140‑1 (MQ=255)                                                  aCCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATg                                                                             >  1:440543/1‑140 (MQ=255)                                                      aaTCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCAt                                                                         >  2:317252/1‑140 (MQ=255)                                                      aaTCGTCTGCTCGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCAt                                                                         >  1:829077/1‑140 (MQ=255)                                                        tCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCAttt                                                                       <  1:150396/140‑1 (MQ=255)                                                           tCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGAcc                                                                                    <  1:220833/124‑1 (MQ=255)                                                           tCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGAcc                                                                                    >  2:220833/1‑124 (MQ=255)                                                             tGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATCTgtctc                                                                  >  1:982981/1‑136 (MQ=255)                                                                   tgGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGAc                                                            <  1:317252/140‑1 (MQ=255)                                                                   tgGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGAc                                                            <  1:518187/140‑1 (MQ=255)                                                                     gTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACgg                                                          >  1:295057/1‑140 (MQ=255)                                                                      tCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGc                                                         <  2:440543/140‑1 (MQ=255)                                                                                tatGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAg                                               >  1:171656/1‑140 (MQ=255)                                                                                      cGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCcgcg                                         >  1:404431/1‑140 (MQ=255)                                                                                      cGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCcgcg                                         >  1:425745/1‑140 (MQ=255)                                                                                          gtggCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATgc                                     >  1:678402/1‑140 (MQ=255)                                                                                          gtggCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATgc                                     >  2:840225/1‑140 (MQ=255)                                                                                          gtggCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATgc                                     >  1:393455/1‑140 (MQ=255)                                                                                                  ggcgcAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGc                             <  1:840225/140‑1 (MQ=255)                                                                                                   gcgcAATACGGCATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCg                            <  2:295057/140‑1 (MQ=255)                                                                                                              cATTAATTACGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGaaa                 <  1:344856/140‑1 (MQ=255)                                                                                                                  aattaCGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGgc             <  2:425745/140‑1 (MQ=255)                                                                                                                   attaCGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGgcg            >  2:598761/1‑140 (MQ=255)                                                                                                                   attaCGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGgcg            <  1:313522/140‑1 (MQ=255)                                                                                                                    ttaCGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGgcgc           <  2:393455/140‑1 (MQ=255)                                                                                                                      ccGCCGTTGCGTTGTTAGGCCCGGCAACCCCCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTg         <  2:975559/139‑1 (MQ=255)                                                                                                                      aCGCCGTTGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTg         >  1:585853/1‑140 (MQ=255)                                                                                                                            ttGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTg         <  1:371363/134‑1 (MQ=255)                                                                                                                            ttGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTg         >  2:371363/1‑134 (MQ=255)                                                                                                                             tGCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACa  >  1:444496/1‑140 (MQ=255)                                                                                                                             ggCGTCGTTAGGCACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTAcg  <  2:678402/139‑2 (MQ=255)                                                                                                                                         |                                                                                                                                AAGGCCGCCAGCTTTACGGTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTATATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGTACGTCAACCACCGCCGATCACATACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACA  >  NC_000913/3211828‑3212091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑