Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,247,179	G→A	W176* (TGG→TGA)	exuR →	DNA‑binding transcriptional repressor ExuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,247,179	0	G	A	100.0%	171.9 / NA	50	W176* (TGG→TGA)	exuR	DNA‑binding transcriptional repressor ExuR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/28);  total (22/28)

GCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑GAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACAC  >  NC_000913/3247042‑3247313                                                                                                                                           |                                                                                                                                       gcgcgcGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTGATAACCCGTACTG‑aaa                                                                                                                                      >  2:896607/1‑140 (MQ=255) gcgcgcGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑aaa                                                                                                                                      >  1:492538/1‑140 (MQ=255)        gcgAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTg                                                                                                                               <  1:42433/140‑1 (MQ=255)          gAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCa                                                                                                                             <  2:469374/140‑1 (MQ=255)             cAATGCTTCCGTGATTCCGAGTGGGA‑TTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGaa                                                                                                                         <  1:302595/140‑1 (MQ=255)                  cTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAAcaca                                                                                                                     <  2:845953/140‑1 (MQ=255)                    tCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACAtt                                                                                                                   <  2:29209/140‑1 (MQ=255)                        tGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGAtt                                                                                                               >  2:262492/1‑140 (MQ=255)                            tCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCg                                                                                                           >  2:1001501/1‑140 (MQ=255)                              cGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTa                                                                                                         <  2:737999/140‑1 (MQ=255)                              cGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTa                                                                                                         <  2:141937/140‑1 (MQ=255)                              cGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTa                                                                                                         <  1:181976/140‑1 (MQ=255)                              cGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTa                                                                                                         <  1:591734/140‑1 (MQ=255)                                aGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTAcc                                                                                                       >  1:298276/1‑140 (MQ=255)                                aGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTAcc                                                                                                       >  1:183543/1‑140 (MQ=255)                                               tCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTgg                                                                                           <  2:876240/137‑1 (MQ=255)                                               tCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTgg                                                                                           >  1:876240/1‑137 (MQ=255)                                                ccACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGtgtg                                                                                       >  2:441173/1‑140 (MQ=255)                                                      tCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGAcc                                                                                 <  1:1001501/140‑1 (MQ=255)                                                       cAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCa                                                                                <  2:492538/140‑1 (MQ=255)                                                          gTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGa                                                                             >  1:975208/1‑140 (MQ=255)                                                              ctctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGAAAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGtgat                                                                                     >  1:647102/1‑129 (MQ=255)                                                              ctctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTGAAAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGtgat                                                                                     <  2:647102/129‑1 (MQ=255)                                                              ctctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCaa                                                                         >  1:165964/1‑140 (MQ=255)                                                                ctGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAAt                                                                       <  1:914766/140‑1 (MQ=255)                                                                          aGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGgcg                                                             <  2:298276/140‑1 (MQ=255)                                                                                      tGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCaaa                                                 >  1:859145/1‑140 (MQ=255)                                                                                        gcggcTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGa                                               >  2:232435/1‑140 (MQ=255)                                                                                        gcggcTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGa                                               >  1:439920/1‑140 (MQ=255)                                                                                           gcTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATcc                                            <  1:537448/140‑1 (MQ=255)                                                                                             tATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTc                                          >  2:190792/1‑140 (MQ=255)                                                                                              aTCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCa                                         <  2:859145/140‑1 (MQ=255)                                                                                                    gAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCAtgctgc                                   >  1:817927/1‑140 (MQ=255)                                                                                                     aaaaaaTGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCAtgctgcc                                  >  1:208365/1‑139 (MQ=255)                                                                                                        aaaTGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAg                               <  1:190792/140‑1 (MQ=255)                                                                                                        aaaTGTGGACCCAGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAg                               <  2:113187/140‑1 (MQ=255)                                                                                                                    aGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTgg                   >  1:228392/1‑140 (MQ=255)                                                                                                                    aGCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTgg                   >  2:50333/1‑140 (MQ=255)                                                                                                                     gCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGgc                  <  2:975208/140‑1 (MQ=255)                                                                                                                     gCGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGgc                  >  1:724654/1‑140 (MQ=255)                                                                                                                      cGTAGTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGgca                 <  2:817927/140‑1 (MQ=255)                                                                                                                          gTCATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCAt             <  2:439920/140‑1 (MQ=255)                                                                                                                            cATAACCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCt           <  1:814735/140‑1 (MQ=255)                                                                                                                                aCCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGaa       <  1:50333/140‑1 (MQ=255)                                                                                                                                aCCCGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGaa       >  1:856343/1‑140 (MQ=255)                                                                                                                                 cccGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGaaa      <  2:183543/140‑1 (MQ=255)                                                                                                                                   cGTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAac    >  2:680029/1‑140 (MQ=255)                                                                                                                                    gTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAaca   <  2:228392/140‑1 (MQ=255)                                                                                                                                    gTACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAaca   <  1:896607/140‑1 (MQ=255)                                                                                                                                     tACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAacac  <  2:29410/140‑1 (MQ=255)                                                                                                                                     tACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAacac  >  1:391051/1‑140 (MQ=255)                                                                                                                                     tACTG‑AAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAacac  >  1:29410/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       GCGCGCGGCGAACAATGCTTCCGTGATTCCGAGTGGGATTTGCAGTTCCACATTCAGGTCGCTCTGGCGACGCAGAACTCCGCCCTGGCGGCTATCGTGGAAAAAATGTGGACCCAGCGTAGTCATAACCCGTACTG‑GAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACAC  >  NC_000913/3247042‑3247313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑