Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,264,994	T→C	V8V (GTA→GTG)	tdcC ←	threonine/serine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,264,994	0	T	C	100.0%	198.7 / NA	56	V8V (GTA→GTG)	tdcC	threonine/serine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/29);  total (27/29)

AAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGTTTTTCTGTT  >  NC_000913/3264859‑3265122                                                                                                                                        |                                                                                                                                 aagaaTCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACaa                                                                                                                              >  2:267806/1‑140 (MQ=255)     aTCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCt                                                                                                                          >  1:490236/1‑140 (MQ=255)      tCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTa                                                                                                                         >  2:177980/1‑140 (MQ=255)       cGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTAt                                                                                                                        <  1:25643/140‑1 (MQ=255)       cGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTAt                                                                                                                        <  2:224860/140‑1 (MQ=255)       cGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTAt                                                                                                                        <  2:125909/140‑1 (MQ=255)       cGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTAt                                                                                                                        <  2:490236/140‑1 (MQ=255)             cAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAg                                                                                                                  <  2:814505/140‑1 (MQ=255)              aGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGt                                                                                                                 >  2:829465/1‑140 (MQ=255)                tCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTAc                                                                                                               >  2:105356/1‑140 (MQ=255)                tCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTAc                                                                                                               >  1:267775/1‑140 (MQ=255)                  cGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTc                                                                                                             >  1:617544/1‑140 (MQ=255)                  cGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTc                                                                                                             <  1:739447/140‑1 (MQ=255)                   gCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCa                                                                                                            <  1:494906/140‑1 (MQ=255)                    ccAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCat                                                                                                           >  2:634423/1‑140 (MQ=255)                       aaaCCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCAtatc                                                                                                        <  1:267806/140‑1 (MQ=255)                                 ggATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCaa                                                                                              <  2:617544/140‑1 (MQ=255)                                 ggATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCaa                                                                                              <  2:720220/140‑1 (MQ=255)                                     aGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGaa                                                                                          <  1:105356/140‑1 (MQ=255)                                                 cACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGaa                                                                                          <  1:452003/128‑1 (MQ=255)                                                 cACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGaa                                                                                          >  2:452003/1‑128 (MQ=255)                                                 cACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGt                                                                              >  1:568384/1‑140 (MQ=255)                                                 cACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGt                                                                              >  2:354084/1‑140 (MQ=255)                                                   ccccGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCa                                                                            >  1:344315/1‑140 (MQ=255)                                                      cGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACg                                                                         <  2:344315/140‑1 (MQ=255)                                                      cGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACg                                                                         >  2:494663/1‑140 (MQ=255)                                                      cGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACg                                                                         >  1:188424/1‑140 (MQ=255)                                                           ccGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGa                                                                        >  1:37798/1‑136 (MQ=255)                                                           ccGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGa                                                                        <  2:37798/136‑1 (MQ=255)                                                            cGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCg                                                                   <  1:829465/140‑1 (MQ=255)                                                                     tACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTga                                                          <  1:32360/140‑1 (MQ=255)                                                                       ccAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTgaga                                                        <  2:535597/140‑1 (MQ=255)                                                                        cAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTgagag                                                       >  1:597241/1‑140 (MQ=255)                                                                          aaCAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGAc                                                     >  1:320323/1‑140 (MQ=255)                                                                          aaCAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGAc                                                     <  2:320323/140‑1 (MQ=255)                                                                           aCAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGAcg                                                    >  1:775543/1‑140 (MQ=255)                                                                              aGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAGCCGGTGATTTGAGAGACGCga                                                 <  1:634423/140‑1 (MQ=255)                                                                                 cTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGaa                                              >  2:379772/1‑140 (MQ=255)                                                                                  tAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGaaa                                             <  1:354084/140‑1 (MQ=255)                                                                                   aaCGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATT‑AGCGTCAACAAAACCGGTGATTTGAGAGACGCGAGAAAGa                                           >  1:425116/1‑140 (MQ=255)                                                                                         cATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGat                                      >  2:241806/1‑140 (MQ=255)                                                                                          aTGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGata                                     <  2:20038/140‑1 (MQ=255)                                                                                           tgtgGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGatat                                    >  2:924897/1‑140 (MQ=255)                                                                                                  tCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTgccgcc                             <  2:750228/140‑1 (MQ=255)                                                                                                      aTTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTgccgcc                             >  1:537510/1‑136 (MQ=255)                                                                                                      aTTTACGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTgccgcc                             <  2:537510/136‑1 (MQ=255)                                                                                                          aCGCCAGGACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAaataa                     >  2:51607/1‑140 (MQ=255)                                                                                                                 gACGATTGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGAc              <  1:241806/140‑1 (MQ=255)                                                                                                                      ttGTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGttt         >  1:249719/1‑140 (MQ=255)                                                                                                                        gTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGttttt       <  1:379772/140‑1 (MQ=255)                                                                                                                        gTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGttttt       <  2:271988/140‑1 (MQ=255)                                                                                                                        gTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGttttt       <  2:188424/140‑1 (MQ=255)                                                                                                                        gTTTTGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAAATAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGttttt       <  2:267775/140‑1 (MQ=255)                                                                                                                            tGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGTTTTTCTGt   >  1:314276/1‑140 (MQ=255)                                                                                                                            tGTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGTTTTTCTGt   >  1:40666/1‑140 (MQ=255)                                                                                                                             gTCTGGCTGGACACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGTTTTTCTGtt  <  1:924897/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 AAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATATCCTATCCTCAACGAATTAATTAAGCGTCAACGAAACCGGTGATTTGAGAGACGCGAGAAAGATCGATATTGCCGCCGGAAATAATACTGACGGTTTTTCTGTT  >  NC_000913/3264859‑3265122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑