Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,484,293	A→G	T26A (ACC→GCC)	yheU →	UPF0270 family protein YheU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,484,293	0	A	G	100.0%	194.0 / NA	55	T26A (ACC→GCC)	yheU	UPF0270 family protein YheU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

CCGCAAATGTGGCTGGAGTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTT  >  NC_000913/3484156‑3484426                                                                                                                                          |                                                                                                                                      ccGCAAATGTGGCTGGAGTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGcc                                                                                                                                     >  1:326361/1‑140 (MQ=255)     aaaTGTGGCTGGAGTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGAtt                                                                                                                                 <  1:710035/140‑1 (MQ=255)     aaaTGTGGCTGGAGTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGAtt                                                                                                                                 <  1:483760/140‑1 (MQ=255)            gCTGGAGTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGAt                                                                                                                                  <  1:890838/132‑1 (MQ=255)            gCTGGAGTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGAt                                                                                                                                  >  2:890838/1‑132 (MQ=255)                gAGTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCAGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACAt                                                                                                                      <  2:176262/140‑1 (MQ=255)                  gTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGa                                                                                                                    <  2:594253/140‑1 (MQ=255)                     aCGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCg                                                                                                                 >  1:291306/1‑140 (MQ=255)                       gCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTa                                                                                                               <  2:326361/140‑1 (MQ=255)                       gCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTa                                                                                                               <  1:62315/140‑1 (MQ=255)                          ttCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTacac                                                                                                            <  2:291306/140‑1 (MQ=255)                           tCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACt                                                                                                           <  1:925939/140‑1 (MQ=255)                               gACTGGATAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGTAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGaa                                                                                                       >  2:462762/1‑140 (MQ=255)                                            cGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCg                                                                                          >  2:1000220/1‑140 (MQ=255)                                              tATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGAc                                                                                        >  1:745125/1‑140 (MQ=255)                                              tATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGAc                                                                                        >  1:497141/1‑140 (MQ=255)                                                   ggAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCaa                                                                                   <  2:745125/140‑1 (MQ=255)                                                    gAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACg                                                                                       <  1:73040/135‑1 (MQ=255)                                                    gAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACg                                                                                       >  2:73040/1‑135 (MQ=255)                                                    gAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCaaa                                                                                  >  2:420798/1‑140 (MQ=255)                                                     aGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAAc                                                                                 <  1:140373/140‑1 (MQ=255)                                                      ggCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACg                                                                                >  1:597227/1‑140 (MQ=255)                                                        cGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGcc                                                                              <  1:67545/140‑1 (MQ=255)                                                        cGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGcc                                                                              >  1:394962/1‑140 (MQ=255)                                                        cGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGcc                                                                              >  2:813085/1‑140 (MQ=255)                                                          aaaTCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAg                                                                            >  2:939552/1‑140 (MQ=255)                                                          aaaTCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAg                                                                            <  1:420798/140‑1 (MQ=255)                                                             tCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTa                                                                         <  1:1000220/140‑1 (MQ=255)                                                             tCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTa                                                                         <  1:813085/140‑1 (MQ=255)                                                                  ttGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTg                                                                    >  1:47833/1‑140 (MQ=255)                                                                     aTTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCgg                                                                 >  2:50907/1‑140 (MQ=255)                                                                       tCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGgag                                                               >  1:839244/1‑140 (MQ=255)                                                                         cGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGaa                                                             >  2:135924/1‑140 (MQ=255)                                                                           tGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGc                                                           >  1:366192/1‑140 (MQ=255)                                                                           tGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGc                                                           >  1:514674/1‑140 (MQ=255)                                                                             gCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCgg                                                         <  1:967321/140‑1 (MQ=255)                                                                               aaGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTg                                                       <  2:514674/140‑1 (MQ=255)                                                                                 gACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCt                                                     <  2:47833/140‑1 (MQ=255)                                                                                    ctctCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGt                                                  <  2:275147/140‑1 (MQ=255)                                                                                    ctctCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGt                                                  <  2:839244/140‑1 (MQ=255)                                                                                    ctctCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGt                                                  <  2:366192/140‑1 (MQ=255)                                                                                      ctcCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTAt                                                >  2:405380/1‑140 (MQ=255)                                                                                         ccccGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGt                                             <  2:486182/140‑1 (MQ=255)                                                                                              aaaCGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAg                                        >  2:319760/1‑140 (MQ=255)                                                                                                            ttAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGa                                >  1:757318/1‑134 (MQ=255)                                                                                                            ttAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGa                                <  2:757318/134‑1 (MQ=255)                                                                                                            ttAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGt                          <  1:135924/140‑1 (MQ=255)                                                                                                            ttAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGt                          <  1:462762/140‑1 (MQ=255)                                                                                                            ttAATTGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGt                          <  1:319760/140‑1 (MQ=255)                                                                                                                ttGAAAGCTTTGTGTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAat                      >  1:663212/1‑140 (MQ=255)                                                                                                                          tgtgTTACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCcgc            >  2:345196/1‑140 (MQ=255)                                                                                                                              ttACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGt                          >  2:829422/1‑122 (MQ=255)                                                                                                                              ttACGTGAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGt                          <  1:829422/122‑1 (MQ=255)                                                                                                                                    gAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGtt  >  1:40415/1‑140 (MQ=255)                                                                                                                                    gAAGGCGCCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGtt  >  1:267272/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CCGCAAATGTGGCTGGAGTCACGCATTCCTGACTGGTTAACAACGTATCTGGAGGCGAAATCATGTTGATTCCGTGGCAAGACCTCTCCCCCGAAACGCTGGAAAATTTAATTGAAAGCTTTGTGTTACGTGAAGGCACCGATTATGGTGAACATGAGCGTACACTTGAACAGAAAGTCGCCGACGTCAAACGCCAGCTACAGTGCGGAGAAGCGGTGCTGGTATGGTCGGAGCTGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTT  >  NC_000913/3484156‑3484426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑