Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,535,847	G→A	P8S (CCA→TCA)	envZ ←	sensory histidine kinase EnvZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,535,847	0	G	A	100.0%	220.0 / NA	63	P8S (CCA→TCA)	envZ	sensory histidine kinase EnvZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/32);  total (31/32)

CGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGT  >  NC_000913/3535712‑3535980                                                                                                                                        |                                                                                                                                      cGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACgag                                                                                                                                   <  2:95945/140‑1 (MQ=255)   tACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGaa                                                                                                                                 >  2:432096/1‑140 (MQ=255)    aCGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAg                                                                                                                                >  1:933710/1‑140 (MQ=255)         aGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCaa                                                                                                                           >  1:588002/1‑140 (MQ=255)         aGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCaa                                                                                                                           >  2:793710/1‑140 (MQ=255)         aGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCaa                                                                                                                           >  1:385001/1‑140 (MQ=255)         aGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGAGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCaa                                                                                                                           <  2:374056/140‑1 (MQ=255)            aCTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCg                                                                                                                        <  2:132651/140‑1 (MQ=255)                tattaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAg                                                                                                                                >  1:552070/1‑128 (MQ=255)                tattaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAg                                                                                                                                <  2:552070/128‑1 (MQ=255)                 attaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCa                                                                                                                   <  1:156597/140‑1 (MQ=255)                 attaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCa                                                                                                                   <  2:286834/140‑1 (MQ=255)                 attaAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCa                                                                                                                   <  1:316108/140‑1 (MQ=255)                      aCTGCTGTAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCtt                                                                                                              >  2:74735/1‑140 (MQ=255)                      aCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCtt                                                                                                              >  2:253724/1‑140 (MQ=255)                        tgctgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTa                                                                                                            >  1:766584/1‑140 (MQ=255)                          ctgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTaga                                                                                                          >  2:566606/1‑140 (MQ=255)                          ctgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTaga                                                                                                          >  2:13654/1‑140 (MQ=255)                          ctgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTaga                                                                                                          >  1:891312/1‑140 (MQ=255)                          ctgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTaga                                                                                                          >  1:892899/1‑140 (MQ=255)                           tgGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTagag                                                                                                         <  2:739615/140‑1 (MQ=255)                             taGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGcc                                                                                                       <  1:74735/139‑1 (MQ=255)                               ggCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTagag                                                                                                         >  1:559547/1‑136 (MQ=255)                               ggCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTagag                                                                                                         <  2:559547/136‑1 (MQ=255)                                gCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTc                                                                                                    <  2:385001/140‑1 (MQ=255)                                gCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTc                                                                                                    <  1:281833/140‑1 (MQ=255)                                  tCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCg                                                                                                  >  1:32298/1‑140 (MQ=255)                                  tCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCg                                                                                                  >  1:136298/1‑140 (MQ=255)                                       aaaaTCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACa                                                                                             <  1:253724/140‑1 (MQ=255)                                        aaaTCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACaa                                                                                            <  1:793710/140‑1 (MQ=255)                                              cgAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGt                                                                                      <  2:93325/140‑1 (MQ=255)                                                   ttCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAATTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGccc                                                                                 <  2:892899/140‑1 (MQ=255)                                                   ttCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGccc                                                                                 <  2:32298/140‑1 (MQ=255)                                                   ttCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGccc                                                                                 <  2:136298/140‑1 (MQ=255)                                                    tCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGccca                                                                                <  2:569783/140‑1 (MQ=255)                                                     cAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccag                                                                               <  2:86685/140‑1 (MQ=255)                                                            accaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccccag                                                                        <  2:851149/140‑1 (MQ=255)                                                             ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTa                                                                                     >  2:82662/1‑126 (MQ=255)                                                             ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTa                                                                                     <  1:82662/126‑1 (MQ=255)                                                             ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccccaga                                                                       <  1:87744/140‑1 (MQ=255)                                                             ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccccaga                                                                       >  1:174358/1‑140 (MQ=255)                                                             ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccccaga                                                                       >  2:95303/1‑140 (MQ=255)                                                             ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccccag                                                                        >  1:193246/1‑139 (MQ=255)                                                             ccaGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccccag                                                                        <  2:193246/139‑1 (MQ=255)                                                              caGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGCACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGcccagaccccagac                                                                      <  2:871467/140‑1 (MQ=255)                                                                               ggCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGt                                                                   <  2:433721/126‑1 (MQ=255)                                                                               ggCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGt                                                                   >  1:433721/1‑126 (MQ=255)                                                                                gCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCgg                                                    <  1:566606/140‑1 (MQ=255)                                                                                 cTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGa                                                   <  2:933710/140‑1 (MQ=255)                                                                                    gCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATgc                                                >  1:45515/1‑140 (MQ=255)                                                                                    gCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATgc                                                >  1:607322/1‑140 (MQ=255)                                                                                    gCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATgc                                                <  1:341004/140‑1 (MQ=255)                                                                                           gCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGAt                                         >  1:48915/1‑140 (MQ=255)                                                                                                    cGATGAGCAATAACGTACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTcca                                <  2:174358/140‑1 (MQ=255)                                                                                                                   tACGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAc                 >  2:358974/1‑140 (MQ=255)                                                                                                                     cGGGCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAcgc               >  2:708454/1‑140 (MQ=255)                                                                                                                      gggCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGAcgcg              >  1:238385/1‑140 (MQ=255)                                                                                                                        gCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGaa            >  2:582785/1‑140 (MQ=255)                                                                                                                        gCAAATGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGaa            >  1:450772/1‑140 (MQ=255)                                                                                                                           aaTGAACTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATc         >  2:985069/1‑140 (MQ=255)                                                                                                                               aaCTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCa     <  1:432096/140‑1 (MQ=255)                                                                                                                               aaCTTCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCa     <  2:45515/140‑1 (MQ=255)                                                                                                                                  ttCGTGACGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGt  >  1:726675/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGT  >  NC_000913/3535712‑3535980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑