Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	531,868	A→G	R118R (CGT→CGC)	allS ←	DNA‑binding transcriptional activator AllS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	531,868	0	A	G	100.0%	172.4 / NA	50	R118R (CGT→CGC)	allS	DNA‑binding transcriptional activator AllS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/21);  total (29/21)

AAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAACGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCTTGGCATACTTTCCAGCCAG  >  NC_000913/531731‑532000                                                                                                                                          |                                                                                                                                     aaGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCg                                                                                                                                    >  2:959276/1‑140 (MQ=255)       tCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCg                                                                                                                                    >  2:379645/1‑134 (MQ=255)              tAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGATGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCAc                                                                                                                       >  2:876179/1‑140 (MQ=255)              tAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCAc                                                                                                                       >  2:576755/1‑140 (MQ=255)               aaaGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACg                                                                                                                      >  1:13495/1‑140 (MQ=255)                     aTTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCa                                                                                                                >  1:809317/1‑140 (MQ=255)                     aTTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCa                                                                                                                >  1:569282/1‑140 (MQ=255)                     aTTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCa                                                                                                                >  2:594672/1‑140 (MQ=255)                             gcgcCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCg                                                                                                        <  2:783858/140‑1 (MQ=255)                              cgcCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGa                                                                                                       <  2:809317/140‑1 (MQ=255)                                       tCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTggg                                                                                              <  2:569282/140‑1 (MQ=255)                                             gACGCCGATAGCCAGCGAAAATCCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGt                                                                                        <  2:396365/140‑1 (MQ=255)                                                  cGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGc                                                                                   <  1:828340/140‑1 (MQ=255)                                                            cGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGTAGGTTGTtga                                                                         >  2:530020/1‑140 (MQ=255)                                                               aaaaCCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTtgatga                                                                      <  1:876179/140‑1 (MQ=255)                                                                aaaCCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGAc                                                                     <  1:594672/140‑1 (MQ=255)                                                                        gTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCa                                                             >  2:157665/1‑140 (MQ=255)                                                                              tAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGc                                                       >  1:824719/1‑140 (MQ=255)                                                                                   aGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTcc                                                  >  2:87151/1‑140 (MQ=255)                                                                                   aGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTcc                                                  >  1:374373/1‑140 (MQ=255)                                                                                   aGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTcc                                                  >  2:704220/1‑140 (MQ=255)                                                                                   aGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTc                                                   <  1:867009/139‑1 (MQ=255)                                                                                   aGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTc                                                   >  2:867009/1‑139 (MQ=255)                                                                                    gTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGTAGGTTGTTGATGACAATATTCACCTGGCGTTCCa                                                 <  2:681094/140‑1 (MQ=255)                                                                                      cccAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACg                                               >  1:861470/1‑140 (MQ=255)                                                                                       ccAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGTAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGc                                              <  1:530020/140‑1 (MQ=255)                                                                                       ccAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGc                                              <  2:374373/140‑1 (MQ=255)                                                                                          gACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCcat                                           >  2:940476/1‑140 (MQ=255)                                                                                          gACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCcat                                           >  2:253847/1‑140 (MQ=255)                                                                                           aCGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCcatc                                          >  1:674218/1‑140 (MQ=255)                                                                                            cGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCcatca                                         <  2:617676/140‑1 (MQ=255)                                                                                             gCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCcatcat                                        <  1:253847/140‑1 (MQ=255)                                                                                             gCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCcatcat                                        <  1:940476/140‑1 (MQ=255)                                                                                               ccATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTc                                      <  1:87151/140‑1 (MQ=255)                                                                                                cATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCAGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCa                                     >  2:894326/1‑140 (MQ=255)                                                                                                         ttGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCa                            >  1:721795/1‑140 (MQ=255)                                                                                                         ttGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCa                            >  1:426819/1‑140 (MQ=255)                                                                                                         ttGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCa                            >  1:347620/1‑140 (MQ=255)                                                                                                          tGTCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAg                           <  2:721795/140‑1 (MQ=255)                                                                                                            tCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCt                         >  2:281227/1‑140 (MQ=255)                                                                                                            tCGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCt                         >  1:719431/1‑140 (MQ=255)                                                                                                             cGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTc                        <  1:959276/140‑1 (MQ=255)                                                                                                             cGGGAGATGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTc                        <  1:704220/140‑1 (MQ=255)                                                                                                                 agaTGTGAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCtt                    >  1:713935/1‑140 (MQ=255)                                                                                                                    tgtgAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCTTGGc                 <  2:347620/140‑1 (MQ=255)                                                                                                                      tgAAACTGGGTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCTTGGCAt               <  2:674218/140‑1 (MQ=255)                                                                                                                              ggTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCTTGGCATACTTTcca       >  2:40097/1‑140 (MQ=255)                                                                                                                              ggTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCTTGGCATACTTTcca       >  1:522076/1‑140 (MQ=255)                                                                                                                               gTAAAGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCTTGGCATACTTTccag      <  1:281227/140‑1 (MQ=255)                                                                                                                                   aGGGGTAGCGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCTTGGCATACTTTccagccag  >  1:475415/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     AAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGACGCCCATATAGATTTGTCGGGAGATGTGAAACTGGGTAAAGGGGTAACGCTCATTCAGCCACGCCAGCAACTGGGCGACGGCCTGGGGGTTGTAGAGCAGGTTGTTGATGACAATATTCACCTGGCGTTCCACGCCATCATTCACCTGTTGCAGCTCGCTTGGCATACTTTCCAGCCAG  >  NC_000913/531731‑532000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑