Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,537,063	A→G	K84K (AAA→AAG)	greB →	transcription elongation factor GreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,537,063	0	A	G	100.0%	175.8 / NA	50	K84K (AAA→AAG)	greB	transcription elongation factor GreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/24);  total (26/24)

CCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCT  >  NC_000913/3536934‑3537197                                                                                                                                  |                                                                                                                                       ccGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAGATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTg                                                                                                                              >  1:706955/1‑140 (MQ=255) ccGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTg                                                                                                                              >  2:12678/1‑140 (MQ=255)  cGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTgg                                                                                                                             >  2:40029/1‑140 (MQ=255)          aaaTGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCgtggg                                                                                                                     <  1:38492/140‑1 (MQ=255)          aaaTGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCgtggg                                                                                                                     <  1:922649/140‑1 (MQ=255)           aaTGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCgtgggt                                                                                                                    <  2:488556/140‑1 (MQ=255)                            taataaAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAacgacg                                                                                                   <  2:706955/140‑1 (MQ=255)                              ataaAAAACGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGAt                                                                                                 >  1:684933/1‑140 (MQ=255)                                 aaaaGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGc                                                                                              <  1:796824/140‑1 (MQ=255)                                   aaGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGt                                                                                            <  2:892527/140‑1 (MQ=255)                                    aGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTg                                                                                           >  2:532306/1‑140 (MQ=255)                                    aGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTg                                                                                           >  2:869493/1‑140 (MQ=255)                                    aGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTg                                                                                           >  1:994560/1‑140 (MQ=255)                                    aGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTg                                                                                           >  1:505141/1‑140 (MQ=255)                                    aGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTg                                                                                           >  1:946493/1‑140 (MQ=255)                                     gCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGa                                                                                          <  1:869493/140‑1 (MQ=255)                                         cTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAacgac                                                                                                    >  2:964787/1‑126 (MQ=255)                                         cTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAacgac                                                                                                    <  1:964787/126‑1 (MQ=255)                                          tGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCAc                                                                                     <  1:477310/140‑1 (MQ=255)                                             gTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGt                                                                                  <  1:605047/140‑1 (MQ=255)                                                      aCCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTAtt                                                                         >  2:291167/1‑140 (MQ=255)                                                            gcgTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGAAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGc                                                                   <  1:180881/140‑1 (MQ=255)                                                                    tATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGa                                                           >  1:61552/1‑140 (MQ=255)                                                                      tctcACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGaaa                                                         >  1:555030/1‑140 (MQ=255)                                                                                tGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCg                                               >  2:936664/1‑140 (MQ=255)                                                                                tGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCg                                               >  1:181017/1‑140 (MQ=255)                                                                                  ccTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTa                                             >  1:875705/1‑140 (MQ=255)                                                                                  ccTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTa                                             >  2:734948/1‑140 (MQ=255)                                                                                  ccTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGAAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTa                                             <  1:291167/140‑1 (MQ=255)                                                                                    tGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTaaa                                           <  2:946493/140‑1 (MQ=255)                                                                                    tGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTaaa                                           >  2:48009/1‑140 (MQ=255)                                                                                          aTCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTAc                                     <  2:555030/140‑1 (MQ=255)                                                                                           tctcAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACa                                    <  2:181017/140‑1 (MQ=255)                                                                                           tctcAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACa                                    <  1:48009/140‑1 (MQ=255)                                                                                            ctcAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACAt                                   <  1:204499/140‑1 (MQ=255)                                                                                                aaaTCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATctct                               <  2:994560/140‑1 (MQ=255)                                                                                                aaaTCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATctct                               >  2:659619/1‑140 (MQ=255)                                                                                                  aTCGTCGATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTAt                             <  2:684933/140‑1 (MQ=255)                                                                                                   tcgtcgATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATc                            <  2:24883/140‑1 (MQ=255)                                                                                                     gtcgATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTa                                      >  2:415767/1‑128 (MQ=255)                                                                                                     gtcgATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTa                                      <  1:415767/128‑1 (MQ=255)                                                                                                     gtcgATTACTCCCCTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGa                          >  1:280027/1‑140 (MQ=255)                                                                                                                 ccTCAGCAGGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGTCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGc              <  1:532306/140‑1 (MQ=255)                                                                                                                    cagcagGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCcg           >  1:17159/1‑140 (MQ=255)                                                                                                                    cagcagGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCcg           >  2:977042/1‑140 (MQ=255)                                                                                                                     agcagGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCcgc          >  1:511792/1‑140 (MQ=255)                                                                                                                      gcagGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCcgcg         <  1:724700/140‑1 (MQ=255)                                                                                                                      gcagGAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCcgcg         <  1:40029/140‑1 (MQ=255)                                                                                                                          gAAGGCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCAtt     >  1:376336/1‑140 (MQ=255)                                                                                                                             ggCAAGGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCt  >  1:289661/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                       CCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCT  >  NC_000913/3536934‑3537197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑