Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,679,687 T→C I90T (ATT→ACT)  kdgK → 2‑dehydro‑3‑deoxygluconokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,679,6870TC100.0% 161.6 / NA 46I90T (ATT→ACT) kdgK2‑dehydro‑3‑deoxygluconokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/23);  total (23/23)

TTAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGATTATCTCTACCTGAG  >  NC_000913/3679554‑3679819
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ttAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTg                                                                                                                                    <  2:422942/136‑1 (MQ=255)
ttAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTg                                                                                                                                    >  1:422942/1‑136 (MQ=255)
ttAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAAc                                                                                                                                >  2:596361/1‑140 (MQ=255)
        tCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCa                                                                                                                        >  1:507196/1‑140 (MQ=255)
          aTTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCAcc                                                                                                                      >  2:158848/1‑140 (MQ=255)
          aTTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCAcc                                                                                                                      >  1:541205/1‑140 (MQ=255)
                 aaCGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGc                                                                                                               >  2:574906/1‑140 (MQ=255)
                   cGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGt                                                                                                             <  2:455732/140‑1 (MQ=255)
                   cGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGt                                                                                                             >  1:455732/1‑140 (MQ=255)
                    ggCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTa                                                                                                            <  2:507196/140‑1 (MQ=255)
                    ggCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTa                                                                                                            <  2:843580/140‑1 (MQ=255)
                      cgcTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTa                                                                                                            >  2:982102/1‑138 (MQ=255)
                      cgcTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTa                                                                                                            <  1:982102/138‑1 (MQ=255)
                        cTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTa                                                                                                            >  2:976778/1‑136 (MQ=255)
                        cTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTa                                                                                                            <  1:976778/136‑1 (MQ=255)
                                ggACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCTACAGCACCGGCGAGCGTACGTTCTactact                                                                                                >  1:600936/1‑140 (MQ=255)
                                ggACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTactact                                                                                                >  1:514922/1‑140 (MQ=255)
                                     gTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCTACAGCACCGGCGAGCGTACGTTCTACTACTGGCgg                                                                                           <  2:600936/140‑1 (MQ=255)
                                     gTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCgg                                                                                           >  1:185856/1‑140 (MQ=255)
                                                   aTGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAgccgccgc                                                                             >  1:766374/1‑140 (MQ=255)
                                                     gCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCa                                                                           >  2:754168/1‑140 (MQ=255)
                                                          aCGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTc                                                                      >  1:35563/1‑140 (MQ=255)
                                                             ccTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTctgg                                                                   >  2:374760/1‑140 (MQ=255)
                                                               tGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTctggct                                                                 <  1:754168/140‑1 (MQ=255)
                                                                 gCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTctggctgg                                                               >  1:102089/1‑140 (MQ=255)
                                                                   aCGGCGAGACCGTTGATACTTCCCTGACCCAACGGATGGAAACCCGTCTGCCGGGCC‑TTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGTTGgaga                                                            <  1:574283/140‑1 (MQ=255)
                                                                               ttGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCt                                                 >  2:606782/1‑140 (MQ=255)
                                                                                 gATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTgc                                               >  1:455832/1‑140 (MQ=255)
                                                                                  aTACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTgcg                                              <  1:606782/140‑1 (MQ=255)
                                                                                      ttCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGa                                          <  2:102089/140‑1 (MQ=255)
                                                                                        cccTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGAtt                                        >  2:320778/1‑140 (MQ=255)
                                                                                            gACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCg                                    <  2:126689/140‑1 (MQ=255)
                                                                                            gACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCg                                    <  1:320778/140‑1 (MQ=255)
                                                                                                  aCGGATGGAAAACCGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGc                              >  2:381970/1‑140 (MQ=255)
                                                                                                               cGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGa                 <  2:4937/140‑1 (MQ=255)
                                                                                                               cGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGa                 <  2:541205/140‑1 (MQ=255)
                                                                                                               cGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGa                 <  2:620176/140‑1 (MQ=255)
                                                                                                               cGTCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGa                 <  1:374760/140‑1 (MQ=255)
                                                                                                                 tCTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGAtt               <  2:766374/140‑1 (MQ=255)
                                                                                                                  cTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAagag                               <  2:536080/123‑1 (MQ=255)
                                                                                                                  cTGCCGGGCCTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAagag                               >  1:536080/1‑123 (MQ=255)
                                                                                                                          ccTTTACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGATTATCTCTAcc      <  1:596361/140‑1 (MQ=255)
                                                                                                                             ttACTACACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGATTATCTCTACCTGa   >  2:877179/1‑140 (MQ=255)
                                                                                                                              tactacACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGATTATCTCTACCTGAg  <  2:154192/140‑1 (MQ=255)
                                                                                                                              tactacACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGATTATCTCTACCTGAg  <  2:35563/140‑1 (MQ=255)
                                                                                                                              tactacACTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGATTATCTCTACCTGAg  <  1:158848/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TTAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCGGAACGAAGCCGCCGCCAAATTCTGGCTGGAGAGTGAGCAGTCTGCGGCGATTTGCGAAGAGCTGGCGAATTTCGATTATCTCTACCTGAG  >  NC_000913/3679554‑3679819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑